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https://doi.org/10.1083/jcb.150.5.989
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論文情報
タイトル
Defects in Axonal Elongation and Neuronal Migration in Mice with Disrupted tau and map1b Genes
著者
Takei, Yosuke
Takei, Yosuke
Teng, Junlin
Teng, Junlin
Harada, Akihiro
Harada, Akihiro
Hirokawa, Nobutaka
Hirokawa, Nobutaka
キーワード等
tau
MAP1B
axon
growth cone
neuronal migration
抄録
Tau and MAP1B are the main members of neuronal microtubule-associated proteins (MAPs), the functions of which have remained obscure because of a putative functional redundancy (Harada, A., K. Oguchi, S. Okabe, J. Kuno, S. Terada, T. Ohshima, R. SatoYoshitake, Y. Takei, T. Noda, and N. Hirokawa. 1994. Nature. 369:488–491; Takei, Y., S. Kondo, A. Harada, S. Inomata, T. Noda, and N. Hirokawa. 1997. J. Cell Biol. 137:1615–1626). To unmask the role of these proteins, we generated double-knockout mice with disrupted tau and map1b genes and compared their phenotypes with those of single-knockout mice. In the analysis of mice with a genetic background of predominantly C57Bl/6J, a hypoplastic commissural axon tract and disorganized neuronal layering were observed in the brains of the tau +/+map1b-/-mice. These phenotypes are markedly more severe in tau -/-map1b-/-double mutants, indicating that tau and MAP1B act in a synergistic fashion. Primary cultures of hippocampal neurons from tau -/-map1b-/-mice showed inhibited axonal elongation. In these cells, a generation of new axons via bundling of microtubules at the neck of the growth cones appeared to be disturbed. Cultured cerebellar neurons from tau -/-map1b-/-mice showed delayed neuronal migration concomitant with suppressed neurite elongation. These findings indicate the cooperative functions of tau and MAP1B in vivo in axonal elongation and neuronal migration as regulators of microtubule oganization.
公開者
The Rockefeller University Press
掲載誌名
Journal of Cell Biology
巻
150
号
5
開始ページ
989
終了ページ
1000
刊行年月
2000-09-04
ISSN
00219525
NCID
AA00694812
DOI
info:doi/10.1083/jcb.150.5.989
URL
http://hdl.handle.net/11094/23081
言語
英語
カテゴリ
学術雑誌論文 Journal Article
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著者版フラグ
publisher
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学術雑誌論文
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text
DCTERMS.bibliographicCitation
Journal of Cell Biology.150(5) P.989-P.1000
DC.title
Defects in Axonal Elongation and Neuronal Migration in Mice with Disrupted tau and map1b Genes
DC.creator
Takei, Yosuke
Teng, Junlin
Harada, Akihiro
Hirokawa, Nobutaka
DC.publisher
The Rockefeller University Press
DC.language" scheme="DCTERMS.RFC1766
英語
DCTERMS.issued" scheme="DCTERMS.W3CDTF
2000-09-04
DC.identifier
info:doi/10.1083/jcb.150.5.989
DC.identifier" scheme="DCTERMS.URI
http://hdl.handle.net/11094/23081
DC.subject
tau
MAP1B
axon
growth cone
neuronal migration
DCTERMS.abstract
Tau and MAP1B are the main members of neuronal microtubule-associated proteins (MAPs), the functions of which have remained obscure because of a putative functional redundancy (Harada, A., K. Oguchi, S. Okabe, J. Kuno, S. Terada, T. Ohshima, R. SatoYoshitake, Y. Takei, T. Noda, and N. Hirokawa. 1994. Nature. 369:488–491; Takei, Y., S. Kondo, A. Harada, S. Inomata, T. Noda, and N. Hirokawa. 1997. J. Cell Biol. 137:1615–1626). To unmask the role of these proteins, we generated double-knockout mice with disrupted tau and map1b genes and compared their phenotypes with those of single-knockout mice. In the analysis of mice with a genetic background of predominantly C57Bl/6J, a hypoplastic commissural axon tract and disorganized neuronal layering were observed in the brains of the tau +/+map1b-/-mice. These phenotypes are markedly more severe in tau -/-map1b-/-double mutants, indicating that tau and MAP1B act in a synergistic fashion. Primary cultures of hippocampal neurons from tau -/-map1b-/-mice showed inhibited axonal elongation. In these cells, a generation of new axons via bundling of microtubules at the neck of the growth cones appeared to be disturbed. Cultured cerebellar neurons from tau -/-map1b-/-mice showed delayed neuronal migration concomitant with suppressed neurite elongation. These findings indicate the cooperative functions of tau and MAP1B in vivo in axonal elongation and neuronal migration as regulators of microtubule oganization.
citation_title
Defects in Axonal Elongation and Neuronal Migration in Mice with Disrupted tau and map1b Genes
citation_author
Takei, Yosuke
Teng, Junlin
Harada, Akihiro
Hirokawa, Nobutaka
citation_publisher
The Rockefeller University Press
citation_language
英語
citation_date
2000-09-04
citation_journal_title
Journal of Cell Biology
citation_volume
150
citation_issue
5
citation_firstpage
989
citation_lastpage
1000
citation_issn
00219525
citation_doi
info:doi/10.1083/jcb.150.5.989
citation_public_url
http://hdl.handle.net/11094/23081
citation_keywords
tau
MAP1B
axon
growth cone
neuronal migration