Artificial tethering of constitutive centromere-associated network proteins induces CENP-A deposition without Knl2 in DT40 cells

Cao, Jing Hui; Hori, Tetsuya; Ariyoshi, Mariko et al.

Journal of Cell Science, 2024, 137(3)

アクセス数:50件（2025-04-20 23:38 集計）

固定URL: https://hdl.handle.net/11094/96426

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論文情報

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タイトル
Artificial tethering of constitutive centromere-associated network proteins induces CENP-A deposition without Knl2 in DT40 cells
著者
著者名
Cao, Jing Hui
NRID
1000070550078 1000070550078
researchmap permalink
thori567 thori567
Scopus Author ID
7402411497 7402411497
著者名
Hori, Tetsuya
NRID
1000080437243 1000080437243
ORCID
0000-0002-1361-2642 0000-0002-1361-2642
researchmap permalink
7000010594 7000010594
Scopus Author ID
7006547610 7006547610
著者名
Ariyoshi, Mariko
NRID
1000060321600 1000060321600
researchmap permalink
fukagawa_tatsuo fukagawa_tatsuo
Scopus Author ID
7003912496 7003912496
著者名
Fukagawa, Tatsuo
キーワード等
CENP-A
CENP-C
Centromere
HJURP
Kinetochore
Knl2
抄録
The kinetochore is an essential structure for chromosome segregation. Although the kinetochore is usually formed on a centromere locus, it can be artificially formed at a non-centromere locus by protein tethering. An artificial kinetochore can be formed by tethering of CENP-C or CENP-I, members of the constitutive centromere-associated network (CCAN). However, how CENP-C or CENP-I recruit the centromere-specific histone CENP-A to form an artificial kinetochore remains unclear. In this study, we analyzed this issue using the tethering assay combined with an auxin-inducible degron (AID)-based knockout method in chicken DT40 cells. We found that tethering of CENP-C or CENP-I induced CENP-A incorporation at the non-centromeric locus in the absence of Knl2 (or MIS18BP1), a component of the Mis18 complex, and that Knl2 tethering recruited CENP-A in the absence of CENP-C. We also showed that CENP-C coimmunoprecipitated with HJURP, independently of Knl2. Considering these results, we propose that CENP-C recruits CENP-A by HJURP binding to form an artificial kinetochore. Our results suggest that CENP-C or CENP-I exert CENP-A recruitment activity, independently of Knl2, for artificial kinetochore formation in chicken DT40 cells. This gives us a new insight into mechanisms for CENP-A incorporation.
出版者
Company of Biologists Ltd
収録物名
Journal of Cell Science
巻(号)
137(3)
刊行年月
2024-02-06
言語
英語
ハンドルURL
https://hdl.handle.net/11094/96426
PISSN
00219533
eISSN
14779137
関連情報 (isVersionOf)
DOI (出版社版)
https://doi.org/10.1242/jcs.261639
アクセス権
open access
出版タイプ
Accepted Manuscript
カテゴリ
学術雑誌論文 Journal Article