Human induced pluripotent stem cell-derived cardiomyocyte patches ameliorate right ventricular function in a rat pressure-overloaded right ventricle model

Watanabe, Takuji; Kawamura, Takuji; Harada, Akima et al.

Journal of Artificial Organs, 2024

アクセス数:1,634件（2025-07-22 21:09 集計）

固定URL: https://hdl.handle.net/11094/100124

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タイトル
Human induced pluripotent stem cell-derived cardiomyocyte patches ameliorate right ventricular function in a rat pressure-overloaded right ventricle model
著者
NRID
1000080838582 1000080838582
researchmap permalink
takuji-watanabe takuji-watanabe
Scopus Author ID
57209196810 57209196810
著者名
Watanabe, Takuji
NRID
1000060839398 1000060839398
researchmap permalink
takuji-kawamura takuji-kawamura
Scopus Author ID
55331768500 55331768500
ORCID
0000-0003-2985-0036 0000-0003-2985-0036
著者名
Kawamura, Takuji
NRID
1000070535426 1000070535426
著者名
Harada, Akima
NRID
1000090624651 1000090624651
Scopus Author ID
57188551084 57188551084
著者名
Taira, Masaki
NRID
1000040645959 1000040645959
researchmap permalink
d-yoshioka d-yoshioka
Scopus Author ID
7003892845 7003892845
著者名
Yoshioka, Daisuke
NRID
1000010507205 1000010507205
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kazuo-shimamura kazuo-shimamura
Scopus Author ID
35969640700 35969640700
著者名
Shimamura, Kazuo
NRID
1000090648932 1000090648932
ORCID
0000-0001-8658-2395 0000-0001-8658-2395
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twosaka2018 twosaka2018
Scopus Author ID
57421862900 57421862900
著者名
Watabe, Tadashi
NRID
1000030370258 1000030370258
著者名
Shimosegawa, Eku
NRID
1000060437316 1000060437316
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takayoshi-ueno takayoshi-ueno
Scopus Author ID
8137931400 8137931400
著者名
Ueno, Takayoshi
NRID
1000070544237 1000070544237
ORCID
0000-0003-0015-6569 0000-0003-0015-6569
researchmap permalink
s-miyagawa3154 s-miyagawa3154
Scopus Author ID
55560624700 55560624700
著者名
Miyagawa, Shigeru
キーワード等
Cardiomyocyte patch
Congenital heart disease
hiPS-CM transplantation
Pressure-overloaded RV failure
RV failure
内容
The version of record of this article, first published in Journal of Artificial Organs, is available online at Publisher’s website: https://doi.org/10.1007/s10047-024-01479-3.
抄録
Right ventricular (RV) failure following surgical repair of congenital heart disease affects survival. Human induced pluripotent stem cell-derived cardiomyocyte (hiPS-CM) sheet transplantation ameliorated left ventricular dysfunction in preclinical studies, indicating its efficacy in RV failure in congenital heart disease. This study aimed to evaluate whether hiPS-CMs could improve RV function in rats with pressure-overloaded RV failure. F344/NJcl-rnu/rnu rats underwent pulmonary artery banding (PAB) via left thoracotomy. Four weeks after PAB, hiPS-CM patch transplantation to the RV was performed in the hiPS-CM group (n = 33), and a sham operation was performed in the sham group (n = 18). We evaluated cardiac catheterization, positron emission tomography data, and pathological results 8 weeks following PAB. RV end-diastolic pressure, the time constant of isovolumic relaxation, and end-diastolic pressure–volume relation were significantly ameliorated in the hiPS-CM group compared with in the sham group. Picrosirius red staining revealed that anti-fibrotic effects were significantly higher in the hiPS-CM group than in the sham group. Von Willebrand factor staining revealed significantly higher myocardial capillary vascular density in the hiPS-CM group than in the sham group. hiPS-CMs were detected in the epicardium 4 weeks after hiPS-CM sheet transplantation. The angiogenic gene expression in the myocardium was significantly higher in the hiPS-CM group than in the sham group. Overall, in rats with pressure-overloaded RV failure, hiPS-CM patch transplantation could improve diastolic function, suppress ventricular fibrosis, and increase capillary density, suggesting that it is a promising treatment for RV failure.
出版者
Springer
収録物名
Journal of Artificial Organs
刊行年月
2024-12-06
言語
英語
ハンドルURL
https://hdl.handle.net/11094/100124
PISSN
14347229
eISSN
16190904
関連情報 (isIdenticalTo)
DOI (出版社版)
https://doi.org/10.1007/s10047-024-01479-3
アクセス権
open access
権利情報
This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ
Version of Record
カテゴリ
学術雑誌論文 Journal Article