Optical histopathology based on the nonlabeling analysis with multiphoton excitation imaging

Matsui, Takahiro

doi:https://doi.org/10.1111/pin.13498

Optical histopathology based on the nonlabeling analysis with multiphoton excitation imaging

Matsui, Takahiro

Pathology International, 2025, 75(1), 1-10

アクセス数:286件（2025-08-18 02:19 集計）

固定URL: https://hdl.handle.net/11094/100191

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タイトル	Optical histopathology based on the nonlabeling analysis with multiphoton excitation imaging
著者	NRID 1000050747037 1000050747037 ORCID 0000-0002-6753-1688 0000-0002-6753-1688 researchmap permalink takahiromatsui takahiromatsui Scopus Author ID 35176152500 35176152500 著者名 Matsui, Takahiro
キーワード等	artificial intelligence
	autofluorescence
	multiphoton excitation imaging
	second harmonic generation
	third harmonic generation
内容	Matsui T. Optical histopathology based on the nonlabeling analysis with multiphoton excitation imaging. Pathology International 75, 1 (2025); https://doi.org/10.1111/pin.13498.
抄録	Histopathological diagnosis is the definitive method for the evaluation of disease status; however, some problems need to be solved, such as invasiveness, time consumption, and difficulty in three-dimensional observation. To overcome these problems, a novel observation method, distinct from conventional histology, using tissue sections and glass slides is desirable. Fluorescence imaging of human tissues with multiphoton excitation imaging (MpEI), which was originally used for intravital imaging in biological research, is a promising method. Label-free MpEI, which requires only near-infrared excitation, can construct images with autofluorescent signals from fresh tissues, as well as nonlinear optical phenomena. It is possible to perform real-time three-dimensional imaging of human tissues without any tissue removal, fixation, or staining. This method has been reported to be useful for histopathological classification in multiple organs and tissues. Moreover, it is very compatible with quantitative image analyses, including artificial intelligence. Based on these characteristics, label-free MpEI has sufficient potential for clinical applications such as in endoscopy and intraoperative rapid diagnosis. The clinical application of label-free MpEI will bring changes not only to histopathology examination but also the clinical bedside and will contribute to the further development of histopathology.
出版者	John Wiley and Sons Inc
収録物名	Pathology International
巻(号)	75(1)
ページ	1-10
刊行年月	2025-01-01
言語	英語
ハンドルURL	https://hdl.handle.net/11094/100191
PISSN	13205463
eISSN	14401827
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1111/pin.13498
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCTERMS.bibliographicCitation	Pathology International. 2025, 75(1), p. 1-10
DC.title	Optical histopathology based on the nonlabeling analysis with multiphoton excitation imaging
DC.creator	Matsui, Takahiro
DC.publisher	John Wiley and Sons Inc
DC.language' scheme='DCTERMS.RFC1766	英語
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2025-01-01
DC.identifier	https://doi.org/10.1111/pin.13498
DC.subject	artificial intelligence
	autofluorescence
	multiphoton excitation imaging
	second harmonic generation
	third harmonic generation
DC.description	Matsui T. Optical histopathology based on the nonlabeling analysis with multiphoton excitation imaging. Pathology International 75, 1 (2025); https://doi.org/10.1111/pin.13498.
DCTERMS.abstract	Histopathological diagnosis is the definitive method for the evaluation of disease status; however, some problems need to be solved, such as invasiveness, time consumption, and difficulty in three-dimensional observation. To overcome these problems, a novel observation method, distinct from conventional histology, using tissue sections and glass slides is desirable. Fluorescence imaging of human tissues with multiphoton excitation imaging (MpEI), which was originally used for intravital imaging in biological research, is a promising method. Label-free MpEI, which requires only near-infrared excitation, can construct images with autofluorescent signals from fresh tissues, as well as nonlinear optical phenomena. It is possible to perform real-time three-dimensional imaging of human tissues without any tissue removal, fixation, or staining. This method has been reported to be useful for histopathological classification in multiple organs and tissues. Moreover, it is very compatible with quantitative image analyses, including artificial intelligence. Based on these characteristics, label-free MpEI has sufficient potential for clinical applications such as in endoscopy and intraoperative rapid diagnosis. The clinical application of label-free MpEI will bring changes not only to histopathology examination but also the clinical bedside and will contribute to the further development of histopathology.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	100191
citation_title	Optical histopathology based on the nonlabeling analysis with multiphoton excitation imaging
citation_author	Matsui, Takahiro
citation_publisher	John Wiley and Sons Inc
citation_language	英語
citation_date	2025-01-01
citation_journal_title	Pathology International
citation_volume	75
citation_issue	1
citation_firstpage	1
citation_lastpage	10
citation_doi	https://doi.org/10.1111/pin.13498
citation_public_url	https://hdl.handle.net/11094/100191

Optical histopathology based on the nonlabeling analysis with multiphoton excitation imaging

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