Genetically encoded bioluminescent glucose indicator for biological research

Tanaka, Rikuto; Sugiura, Kazunori; Osabe, Kenji; Hattori, Mitsuru; Nagai, Takeharu

doi:https://doi.org/10.1016/j.bbrc.2024.151092

Genetically encoded bioluminescent glucose indicator for biological research

Tanaka, Rikuto; Sugiura, Kazunori; Osabe, Kenji et al.

Biochemical and Biophysical Research Communications, 2025, 742, 151092

アクセス数:554件（2025-07-18 12:12 集計）

固定URL: https://hdl.handle.net/11094/100214

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タイトル	Genetically encoded bioluminescent glucose indicator for biological research
著者	著者名 Tanaka, Rikuto
	著者名 Sugiura, Kazunori
	NRID 1000060751352 1000060751352 著者名 Osabe, Kenji
	NRID 1000020589858 1000020589858 researchmap permalink mhatto mhatto Scopus Author ID 7401882679 7401882679 ORCID 0000-0003-1189-6554 0000-0003-1189-6554 著者名 Hattori, Mitsuru
	NRID 1000020311350 1000020311350 ORCID 0000-0003-2650-9895 0000-0003-2650-9895 researchmap permalink ng1 ng1 Scopus Author ID 7403433377 7403433377 著者名 Nagai, Takeharu
キーワード等	Bioluminescence imaging
	Genetically encoded bioluminescent indicator
	Glucose metabolism
内容	Tanaka R., Sugiura K., Osabe K., et al. Genetically encoded bioluminescent glucose indicator for biological research. Biochemical and Biophysical Research Communications 742, 151092 (2025); https://doi.org/10.1016/j.bbrc.2024.151092.
抄録	Glucose is an essential energy source in living cells and is involved in various phenomena. To understand the roles of glucose, measuring cellular glucose levels is important. Here, we developed a bioluminescent glucose indicator called LOTUS-Glc. Unlike fluorescence, bioluminescence doesn't require excitation light when imaging. Using LOTUS-Glc, we demonstrated drug effect evaluation, concurrent use with the optogenetic tool in HEK293T cells, and the measurement of light-dependent glucose fluctuations in plant-derived protoplasts. LOTUS-Glc would be a useful tool for understanding the roles of glucose in living organisms.
出版者	Elsevier B.V.
収録物名	Biochemical and Biophysical Research Communications
巻(号)	742
ページ	151092
刊行年月	2025-01-01
言語	英語
ハンドルURL	https://hdl.handle.net/11094/100214
PISSN	0006291X
eISSN	10902104
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1016/j.bbrc.2024.151092
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCTERMS.bibliographicCitation	Biochemical and Biophysical Research Communications. 2025, 742, p. 151092
DC.title	Genetically encoded bioluminescent glucose indicator for biological research
DC.creator	Tanaka, Rikuto
	Sugiura, Kazunori
	Osabe, Kenji
	Hattori, Mitsuru
	Nagai, Takeharu
DC.publisher	Elsevier B.V.
DC.language' scheme='DCTERMS.RFC1766	英語
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2025-01-01
DC.identifier	https://doi.org/10.1016/j.bbrc.2024.151092
DC.subject	Bioluminescence imaging
	Genetically encoded bioluminescent indicator
	Glucose metabolism
DC.description	Tanaka R., Sugiura K., Osabe K., et al. Genetically encoded bioluminescent glucose indicator for biological research. Biochemical and Biophysical Research Communications 742, 151092 (2025); https://doi.org/10.1016/j.bbrc.2024.151092.
DCTERMS.abstract	Glucose is an essential energy source in living cells and is involved in various phenomena. To understand the roles of glucose, measuring cellular glucose levels is important. Here, we developed a bioluminescent glucose indicator called LOTUS-Glc. Unlike fluorescence, bioluminescence doesn't require excitation light when imaging. Using LOTUS-Glc, we demonstrated drug effect evaluation, concurrent use with the optogenetic tool in HEK293T cells, and the measurement of light-dependent glucose fluctuations in plant-derived protoplasts. LOTUS-Glc would be a useful tool for understanding the roles of glucose in living organisms.
DC.rights	This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
DC.format	100214
citation_title	Genetically encoded bioluminescent glucose indicator for biological research
citation_author	Tanaka, Rikuto
	Sugiura, Kazunori
	Osabe, Kenji
	Hattori, Mitsuru
	Nagai, Takeharu
citation_publisher	Elsevier B.V.
citation_language	英語
citation_date	2025-01-01
citation_journal_title	Biochemical and Biophysical Research Communications
citation_volume	742
citation_firstpage	151092
citation_doi	https://doi.org/10.1016/j.bbrc.2024.151092
citation_public_url	https://hdl.handle.net/11094/100214

Genetically encoded bioluminescent glucose indicator for biological research

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