¹³C-metabolic flux analysis of respiratory chain disrupted strain ΔndhF1 of Synechocystis sp. PCC 6803

Wada, Keisuke; Toya, Yoshihiro; Matsuda, Fumio; Shimizu, Hiroshi

doi:https://doi.org/10.1007/s12010-024-05138-4

¹³C-metabolic flux analysis of respiratory chain disrupted strain ΔndhF1 of Synechocystis sp. PCC 6803

Wada, Keisuke; Toya, Yoshihiro; Matsuda, Fumio et al.

Applied Biochemistry and Biotechnology, 2025

アクセス数:587件（2025-07-18 10:18 集計）

固定URL: https://hdl.handle.net/11094/100323

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タイトル	¹³C-metabolic flux analysis of respiratory chain disrupted strain ΔndhF1 of Synechocystis sp. PCC 6803
著者	著者名 Wada, Keisuke
	NRID 1000070582162 1000070582162 ORCID 0000-0001-9670-6961 0000-0001-9670-6961 researchmap permalink ytoya ytoya Scopus Author ID 34572737200 34572737200 著者名 Toya, Yoshihiro
	NRID 1000050462734 1000050462734 ORCID 0000-0003-1091-778X 0000-0003-1091-778X researchmap permalink fumio_matsuda fumio_matsuda Scopus Author ID 7202165617 7202165617 著者名 Matsuda, Fumio
	NRID 1000000226250 1000000226250 ORCID 0000-0002-8986-0861 0000-0002-8986-0861 researchmap permalink hiroshi_shimizu hiroshi_shimizu Scopus Author ID 55230249300 55230249300 著者名 Shimizu, Hiroshi
内容	The version of record of this article, first published in Applied Biochemistry and Biotechnology, is available online at Publisher’s website: https://doi.org/s12010-024-05138-4.
抄録	Cyanobacteria are advantageous hosts for industrial applications toward achieving sustainable society due to their unique and superior properties such as atmospheric CO2 fixation via photosynthesis. However, cyanobacterial productivities tend to be weak compared to heterotrophic microbes. To enhance them, it is necessary to understand the fundamental metabolic mechanisms unique to cyanobacteria. In cyanobacteria, NADPH and ATP regenerated by linear and cyclic electron transfers using light energy are consumed by CO2 fixation in a central metabolic pathway. The previous study demonstrated that the strain deleted a part of respiratory chain complex (ΔndhF1) perturbed NADPH levels and photosynthetic activity in Synechocystis sp. PCC 6803. It is expected that disruption of ndhF1 would result in a decrease in the function of cyclic electron transfer, which controls the ATP/NAD(P)H production ratio properly. In this study, we evaluated the effects of ndhF1 deletion on central metabolism and photosynthesis by 13C-metabolic flux analysis. As results of culturing the control and ΔndhF1 strains in a medium containing [1,2-13C] glucose and estimating the flux distribution, CO2 fixation rate by RuBisCO was decreased to be less than half in the ΔndhF1 strain. In addition, the regeneration rate of NAD(P)H and ATP by the photosystem, which can be estimated from the flux distribution, also decreased to be less than half in the ΔndhF1 strain, whereas no significant difference was observed in ATP/NAD(P)H production ratio between the control and the ΔndhF1 strains. Our result suggests that the ratio of utilization of cyclic electron transfer is not reduced in the ΔndhF1 strain unexpectedly.
出版者	Springer Science and Business Media LLC
収録物名	Applied Biochemistry and Biotechnology
刊行年月	2025-01-15
言語	英語
ハンドルURL	https://hdl.handle.net/11094/100323
PISSN	02732289
eISSN	15590291
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1007/s12010-024-05138-4
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCTERMS.bibliographicCitation	Applied Biochemistry and Biotechnology. 2025
DC.title	¹³C-metabolic flux analysis of respiratory chain disrupted strain ΔndhF1 of Synechocystis sp. PCC 6803
DC.creator	Wada, Keisuke
	Toya, Yoshihiro
	Matsuda, Fumio
	Shimizu, Hiroshi
DC.publisher	Springer Science and Business Media LLC
DC.language' scheme='DCTERMS.RFC1766	英語
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2025-01-15
DC.identifier	https://doi.org/10.1007/s12010-024-05138-4
DC.description	The version of record of this article, first published in Applied Biochemistry and Biotechnology, is available online at Publisher’s website: https://doi.org/s12010-024-05138-4.
DCTERMS.abstract	Cyanobacteria are advantageous hosts for industrial applications toward achieving sustainable society due to their unique and superior properties such as atmospheric CO2 fixation via photosynthesis. However, cyanobacterial productivities tend to be weak compared to heterotrophic microbes. To enhance them, it is necessary to understand the fundamental metabolic mechanisms unique to cyanobacteria. In cyanobacteria, NADPH and ATP regenerated by linear and cyclic electron transfers using light energy are consumed by CO2 fixation in a central metabolic pathway. The previous study demonstrated that the strain deleted a part of respiratory chain complex (ΔndhF1) perturbed NADPH levels and photosynthetic activity in Synechocystis sp. PCC 6803. It is expected that disruption of ndhF1 would result in a decrease in the function of cyclic electron transfer, which controls the ATP/NAD(P)H production ratio properly. In this study, we evaluated the effects of ndhF1 deletion on central metabolism and photosynthesis by 13C-metabolic flux analysis. As results of culturing the control and ΔndhF1 strains in a medium containing [1,2-13C] glucose and estimating the flux distribution, CO2 fixation rate by RuBisCO was decreased to be less than half in the ΔndhF1 strain. In addition, the regeneration rate of NAD(P)H and ATP by the photosystem, which can be estimated from the flux distribution, also decreased to be less than half in the ΔndhF1 strain, whereas no significant difference was observed in ATP/NAD(P)H production ratio between the control and the ΔndhF1 strains. Our result suggests that the ratio of utilization of cyclic electron transfer is not reduced in the ΔndhF1 strain unexpectedly.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	100323
citation_title	¹³C-metabolic flux analysis of respiratory chain disrupted strain ΔndhF1 of Synechocystis sp. PCC 6803
citation_author	Wada, Keisuke
	Toya, Yoshihiro
	Matsuda, Fumio
	Shimizu, Hiroshi
citation_publisher	Springer Science and Business Media LLC
citation_language	英語
citation_date	2025-01-15
citation_journal_title	Applied Biochemistry and Biotechnology
citation_doi	https://doi.org/10.1007/s12010-024-05138-4
citation_public_url	https://hdl.handle.net/11094/100323

¹³C-metabolic flux analysis of respiratory chain disrupted strain ΔndhF1 of Synechocystis sp. PCC 6803

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