Photoperiodic plasticity of pigment-dispersing factor immunoreactive fibers projecting toward prothoracicotropic hormone neurons in flesh fly Sarcophaga similis larvae

Ohe, Yutaro; Hasebe, Masaharu; Hamanaka, Yoshitaka; Goto, G. Shin; Shiga, Sakiko

doi:https://doi.org/10.1007/s00359-024-01729-y

Photoperiodic plasticity of pigment-dispersing factor immunoreactive fibers projecting toward prothoracicotropic hormone neurons in flesh fly Sarcophaga similis larvae

Ohe, Yutaro; Hasebe, Masaharu; Hamanaka, Yoshitaka et al.

Journal of Comparative Physiology A, 2025

アクセス数:293件（2025-09-07 16:30 集計）

固定URL: https://hdl.handle.net/11094/100330

閲覧可能ファイル

ファイル	フォーマット	利用条件	サイズ	閲覧回数	利用開始日	説明	information
JCompPhysiolA_s00359-024-01729-y	pdf	なし	3.87 MB	23	2025.02.14

Creative Commons : 表示

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

タイトル	Photoperiodic plasticity of pigment-dispersing factor immunoreactive fibers projecting toward prothoracicotropic hormone neurons in flesh fly Sarcophaga similis larvae
著者	著者名 Ohe, Yutaro
	NRID 1000040802822 1000040802822 著者名 Hasebe, Masaharu
	NRID 1000010647572 1000010647572 researchmap permalink 7_7_7 7_7_7 Scopus Author ID 36769083600 36769083600 著者名 Hamanaka, Yoshitaka
	NRID 1000070347483 1000070347483 著者名 Goto, G. Shin
	NRID 1000090254383 1000090254383 researchmap permalink read0182838 read0182838 Scopus Author ID 7006284711 7006284711 著者名 Shiga, Sakiko
キーワード等	PDF
	PTTH
	s-LNv
	Varicosity
	Glutamate
	sNPF
内容	The version of record of this article, first published in Journal of Comparative Physiology A, is available online at Publisher’s website: https://doi.org/10.1007/s00359-024-01729-y.
抄録	Larvae of the flesh fly, Sarcophaga similis exhibit photoperiodic responses to control pupal diapause. Although the external coincidence model is applicable to S. similis photoperiodism, it remains unknown how the circadian clock system integrates day-length information. To explore the mechanisms, we examined the neural circuitry involving circadian clock lateral neurons (LNs) and prothoracicotropic hormone (PTTH) neurons. We also examined the photoperiodic effects on LN-fiber patterns in third-instar S. similis larvae. Immunohistochemistry showed that the clock protein PERIOD and the neuropeptide pigment-dispersing factor (PDF) were co-localized in four cells per brain hemisphere, and we named these PDF-LNs of S. similis. Single-cell polymerase chain reaction of backfilled neurons from the ring gland showed that two pairs of pars lateralis neurons with contralateral axons (PL-c neurons) to the ring gland expressed ptth. Double labeling with immunohistochemistry and backfills revealed that PDF-immunoreactive varicose fibers projected close to fibers from PL-c neurons. short neuropeptide f (snpf) receptor and glutamate-gated chloride channel but not pdf receptor were expressed in PL-c neurons. sNPF and L-glutamate but not PDF acutely inhibited the spontaneous firing activity of PL-c neurons. The number of PDF-immunoreactive varicosities of PDF-LNs in the dorsal protocerebrum was significantly higher under short-day than that under long-day conditions in a time-dependent manner. These results suggest that sNPF and/or glutamate signaling to PTTH neurons and PDF-LNs form a potential neural circuity for the photoperiodic control of pupal diapause and that photoperiod modifies the connectivity strength between PDF-LNs and their post- or pre-neurons in the circuitry.
出版者	Springer Science and Business Media LLC
収録物名	Journal of Comparative Physiology A
刊行年月	2025-01-15
言語	英語
ハンドルURL	https://hdl.handle.net/11094/100330
PISSN	03407594
eISSN	14321351
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1007/s00359-024-01729-y
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCTERMS.bibliographicCitation	Journal of Comparative Physiology A. 2025
DC.title	Photoperiodic plasticity of pigment-dispersing factor immunoreactive fibers projecting toward prothoracicotropic hormone neurons in flesh fly Sarcophaga similis larvae
DC.creator	Ohe, Yutaro
	Hasebe, Masaharu
	Hamanaka, Yoshitaka
	Goto, G. Shin
	Shiga, Sakiko
DC.publisher	Springer Science and Business Media LLC
DC.language' scheme='DCTERMS.RFC1766	英語
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2025-01-15
DC.identifier	https://doi.org/10.1007/s00359-024-01729-y
DC.subject	PDF
	PTTH
	s-LNv
	Varicosity
	Glutamate
	sNPF
DC.description	The version of record of this article, first published in Journal of Comparative Physiology A, is available online at Publisher’s website: https://doi.org/10.1007/s00359-024-01729-y.
DCTERMS.abstract	Larvae of the flesh fly, Sarcophaga similis exhibit photoperiodic responses to control pupal diapause. Although the external coincidence model is applicable to S. similis photoperiodism, it remains unknown how the circadian clock system integrates day-length information. To explore the mechanisms, we examined the neural circuitry involving circadian clock lateral neurons (LNs) and prothoracicotropic hormone (PTTH) neurons. We also examined the photoperiodic effects on LN-fiber patterns in third-instar S. similis larvae. Immunohistochemistry showed that the clock protein PERIOD and the neuropeptide pigment-dispersing factor (PDF) were co-localized in four cells per brain hemisphere, and we named these PDF-LNs of S. similis. Single-cell polymerase chain reaction of backfilled neurons from the ring gland showed that two pairs of pars lateralis neurons with contralateral axons (PL-c neurons) to the ring gland expressed ptth. Double labeling with immunohistochemistry and backfills revealed that PDF-immunoreactive varicose fibers projected close to fibers from PL-c neurons. short neuropeptide f (snpf) receptor and glutamate-gated chloride channel but not pdf receptor were expressed in PL-c neurons. sNPF and L-glutamate but not PDF acutely inhibited the spontaneous firing activity of PL-c neurons. The number of PDF-immunoreactive varicosities of PDF-LNs in the dorsal protocerebrum was significantly higher under short-day than that under long-day conditions in a time-dependent manner. These results suggest that sNPF and/or glutamate signaling to PTTH neurons and PDF-LNs form a potential neural circuity for the photoperiodic control of pupal diapause and that photoperiod modifies the connectivity strength between PDF-LNs and their post- or pre-neurons in the circuitry.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	100330
citation_title	Photoperiodic plasticity of pigment-dispersing factor immunoreactive fibers projecting toward prothoracicotropic hormone neurons in flesh fly Sarcophaga similis larvae
citation_author	Ohe, Yutaro
	Hasebe, Masaharu
	Hamanaka, Yoshitaka
	Goto, G. Shin
	Shiga, Sakiko
citation_publisher	Springer Science and Business Media LLC
citation_language	英語
citation_date	2025-01-15
citation_journal_title	Journal of Comparative Physiology A
citation_doi	https://doi.org/10.1007/s00359-024-01729-y
citation_public_url	https://hdl.handle.net/11094/100330

Photoperiodic plasticity of pigment-dispersing factor immunoreactive fibers projecting toward prothoracicotropic hormone neurons in flesh fly Sarcophaga similis larvae

閲覧可能ファイル

論文情報

論文詳細を表示