Enhanced Discriminability of Viral Vectors in Viscous Nanopores

Tsutsui, Makusu; Tsunekawa, Yuji; Wada, Mikako et al.

Small Methods, 2025, 2401321

アクセス数:46件（2025-05-14 13:46 集計）

固定URL: https://hdl.handle.net/11094/100348

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論文情報

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タイトル
Enhanced Discriminability of Viral Vectors in Viscous Nanopores
著者
NRID
1000050546596 1000050546596
ORCID
0000-0002-4552-1163 0000-0002-4552-1163
researchmap permalink
makusu makusu
Scopus Author ID
8978905800 8978905800
著者名
Tsutsui, Makusu
著者名
Tsunekawa, Yuji
NRID
1000050810090 1000050810090
著者名
Wada, Mikako
NRID
1000020781347 1000020781347
著者名
Arima, Akihide
著者名
Onodera, Azusa
著者名
Nishina, Masumi
著者名
Nagoya, Miho
NRID
1000030183916 1000030183916
著者名
Baba, Yoshinobu
NRID
1000020092546 1000020092546
researchmap permalink
read0014173 read0014173
著者名
Kawai, Tomoji
NRID
1000000326828 1000000326828
著者名
Okada, Takashi
キーワード等
electrochemistry
virus vector
gene therapy
nanofluidics
nanopore
内容
Tsutsui M., Tsunekawa Y., Wada M., et al. Enhanced Discriminability of Viral Vectors in Viscous Nanopores. Small Methods , (2025); https://doi.org/10.1002/smtd.202401321.
抄録
Achieving safe and efficient gene therapy hinges upon the inspection of genomes enclosed within individual nano-carriers to mitigate potential health risks associated with empty or fragment-filled vectors. Here solid-state nanopore sensing is reported for identifications of intermediate adeno-associated virus (AAV) vectors in liquid. The method exploits the phenomenon of translocation slowdown induced by the viscosity of salt water-organic mixtures. This enables real-time ionic current measurements allowing precise tracking of the electroosmotic flow-driven motions of recombinant AAV vectors in a nanopore. The resulting ionic signals facilitate discrimination between replicative intermediates carrying ssDNA fragments and its full vector counterparts based on genome length-derived subtle nanometer differences in the viral diameters. This rapid and non-destructive means of genome analysis within virus capsids provides a promising avenue toward a robust methodology for ensuring the integrity of AAV vectors before administration.
出版者
John Wiley and Sons Inc
収録物名
Small Methods
ページ
2401321
刊行年月
2025-01-02
言語
英語
ハンドルURL
https://hdl.handle.net/11094/100348
eISSN
23669608
関連情報 (isIdenticalTo)
DOI (出版社版)
https://doi.org/10.1002/smtd.202401321
アクセス権
open access
権利情報
This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
出版タイプ
Version of Record
カテゴリ
学術雑誌論文 Journal Article