Ratiometric bioluminescent detection of Cu(II) ion based on differences in enzymatic reaction kinetics of two luciferase variants

Wu, Ti; Hossain, Md Nadim; Hattori, Mitsuru; Nagai, Takeharu

doi:https://doi.org/10.1016/j.talanta.2025.127576

Ratiometric bioluminescent detection of Cu(II) ion based on differences in enzymatic reaction kinetics of two luciferase variants

Wu, Ti; Hossain, Md Nadim; Hattori, Mitsuru et al.

Talanta, 2025, 287, 127576

アクセス数:431件（2025-07-18 11:38 集計）

固定URL: https://hdl.handle.net/11094/100399

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タイトル	Ratiometric bioluminescent detection of Cu(II) ion based on differences in enzymatic reaction kinetics of two luciferase variants
著者	著者名 Wu, Ti
	著者名 Hossain, Md Nadim
	NRID 1000020589858 1000020589858 researchmap permalink mhatto mhatto Scopus Author ID 7401882679 7401882679 ORCID 0000-0003-1189-6554 0000-0003-1189-6554 著者名 Hattori, Mitsuru
	NRID 1000020311350 1000020311350 ORCID 0000-0003-2650-9895 0000-0003-2650-9895 researchmap permalink ng1 ng1 Scopus Author ID 7403433377 7403433377 著者名 Nagai, Takeharu
キーワード等	Bioluminescence
	BRET
	Cu²⁺
	Detection
	NanoLuc
	Smartphone
	Water
内容	Wu T., Hossain M.N., Hattori M., et al. Ratiometric bioluminescent detection of Cu(II) ion based on differences in enzymatic reaction kinetics of two luciferase variants. Talanta 287, 127576 (2025); https://doi.org/10.1016/j.talanta.2025.127576.
抄録	Heavy metal contamination in water bodies has raised global concerns due to its significant threats to both public health and ecosystem. Copper (Cu), one of the most widely used metals, is also an essential trace element in physiological systems. Excessive intake of Cu from water can cause toxicity, potentially resulting in serious health risks. Ensuring water safety necessitates the critical detection of copper ion (Cu²⁺). Here, we report a ratiometric bioluminescent detection method for Cu²⁺, DERK-Cu(II), which is based on the Distinct Enzymatic Reaction Kinetics of two luciferase variants. In DERK-Cu(II), the blue luminescent luciferase exhibits lower catalytic efficiency than the green luminescent luciferase, thus it is less inhibited by Cu²⁺. Consequently, the luminescence color of their mixture is dependent on the Cu²⁺ concentrations, enabling us to find an optimal mixing ratio at which luminescence color changes evidently from green to blue. Building on this, we established a quantitative detection method for Cu²⁺ using a smartphone and successfully measured the Cu²⁺ concentrations in several water samples. The method we developed, using the difference in reaction kinetics of two enzymes with the same substrate specificity, would become a versatile approach applicable to the development of other enzyme-based indicators.
出版者	Elsevier B.V.
収録物名	Talanta
巻(号)	287
ページ	127576
刊行年月	2025-05-15
言語	英語
ハンドルURL	https://hdl.handle.net/11094/100399
PISSN	00399140
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1016/j.talanta.2025.127576
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCTERMS.bibliographicCitation	Talanta. 2025, 287, p. 127576
DC.title	Ratiometric bioluminescent detection of Cu(II) ion based on differences in enzymatic reaction kinetics of two luciferase variants
DC.creator	Wu, Ti
	Hossain, Md Nadim
	Hattori, Mitsuru
	Nagai, Takeharu
DC.publisher	Elsevier B.V.
DC.language' scheme='DCTERMS.RFC1766	英語
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2025-05-15
DC.identifier	https://doi.org/10.1016/j.talanta.2025.127576
DC.subject	Bioluminescence
	BRET
	Cu²⁺
	Detection
	NanoLuc
	Smartphone
	Water
DC.description	Wu T., Hossain M.N., Hattori M., et al. Ratiometric bioluminescent detection of Cu(II) ion based on differences in enzymatic reaction kinetics of two luciferase variants. Talanta 287, 127576 (2025); https://doi.org/10.1016/j.talanta.2025.127576.
DCTERMS.abstract	Heavy metal contamination in water bodies has raised global concerns due to its significant threats to both public health and ecosystem. Copper (Cu), one of the most widely used metals, is also an essential trace element in physiological systems. Excessive intake of Cu from water can cause toxicity, potentially resulting in serious health risks. Ensuring water safety necessitates the critical detection of copper ion (Cu²⁺). Here, we report a ratiometric bioluminescent detection method for Cu²⁺, DERK-Cu(II), which is based on the Distinct Enzymatic Reaction Kinetics of two luciferase variants. In DERK-Cu(II), the blue luminescent luciferase exhibits lower catalytic efficiency than the green luminescent luciferase, thus it is less inhibited by Cu²⁺. Consequently, the luminescence color of their mixture is dependent on the Cu²⁺ concentrations, enabling us to find an optimal mixing ratio at which luminescence color changes evidently from green to blue. Building on this, we established a quantitative detection method for Cu²⁺ using a smartphone and successfully measured the Cu²⁺ concentrations in several water samples. The method we developed, using the difference in reaction kinetics of two enzymes with the same substrate specificity, would become a versatile approach applicable to the development of other enzyme-based indicators.
DC.rights	This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
DC.format	100399
citation_title	Ratiometric bioluminescent detection of Cu(II) ion based on differences in enzymatic reaction kinetics of two luciferase variants
citation_author	Wu, Ti
	Hossain, Md Nadim
	Hattori, Mitsuru
	Nagai, Takeharu
citation_publisher	Elsevier B.V.
citation_language	英語
citation_date	2025-05-15
citation_journal_title	Talanta
citation_volume	287
citation_firstpage	127576
citation_doi	https://doi.org/10.1016/j.talanta.2025.127576
citation_public_url	https://hdl.handle.net/11094/100399

Ratiometric bioluminescent detection of Cu(II) ion based on differences in enzymatic reaction kinetics of two luciferase variants

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