学術雑誌論文

学術雑誌論文

Science Advances. 2025, 11(4), p. eadp4750

Creating coveted bioluminescence colors for simultaneous multi-color bioimaging

American Association for the Advancement of Science

英語

2025-01-24

https://doi.org/10.1126/sciadv.adp4750

Hattori M., Wazawa T., Orioka M., et al. Creating coveted bioluminescence colors for simultaneous multi-color bioimaging. Science Advances 11, eadp4750 (2025); https://doi.org/10.1126/sciadv.adp4750.

Bioluminescence, an optical marker that does not require excitation by light, allows researchers to simultaneously observe multiple targets, each exhibiting a different color. Notably, the colors of the bioluminescent proteins must sufficiently vary to enable simultaneous detection. Here, we aimed to introduce a method that can be used to expand the color variation by tuning dual-acceptor bioluminescence resonance energy transfer. Using this approach, we could visualize multiple targets with up to 20 colors through single-shot acquisition using a color complementary metal-oxide semiconductor camera. Overall, this method enables simple and simultaneous observation of multiple biological targets and phenomena.

This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

100434

Creating coveted bioluminescence colors for simultaneous multi-color bioimaging

American Association for the Advancement of Science

英語

2025-01-24

Science Advances

11

4

eadp4750

https://doi.org/10.1126/sciadv.adp4750

https://hdl.handle.net/11094/100434