DNA Flow Cytometry Analysis in Body Cavity Fluids Using Liquid-Based Cytology

Ryu, Ayumi; Honma, Keiichiro; Tsuzaki, Sayoko; Yoshioka, Risa; Tanada, Satoshi; Yamamoto, Takashi; Satomi, Hidetoshi; Nagata, Shigenori; Yamasaki, Tomoyuki; Ohue, Masayuki

doi:https://doi.org/10.1002/dc.25451

DNA Flow Cytometry Analysis in Body Cavity Fluids Using Liquid-Based Cytology

Ryu, Ayumi; Honma, Keiichiro; Tsuzaki, Sayoko et al.

Diagnostic Cytopathology, 2025

アクセス数:799件（2026-02-16 00:34 集計）

固定URL: https://hdl.handle.net/11094/100561

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タイトル	DNA Flow Cytometry Analysis in Body Cavity Fluids Using Liquid-Based Cytology
著者	ORCID 0000-0002-7834-5223 0000-0002-7834-5223 著者名 Ryu, Ayumi
	著者名 Honma, Keiichiro
	著者名 Tsuzaki, Sayoko
	著者名 Yoshioka, Risa
	著者名 Tanada, Satoshi
	著者名 Yamamoto, Takashi
	著者名 Satomi, Hidetoshi
	著者名 Nagata, Shigenori
	著者名 Yamasaki, Tomoyuki
	NRID 1000040294095 1000040294095 ORCID 0000-0001-9134-3292 0000-0001-9134-3292 著者名 Ohue, Masayuki
キーワード等	body cavity fluids
	DNA
	flow cytometry
	liquid-based cytology
内容	This is the peer reviewed version of the following article: Ryu A., Honma K., Tsuzaki S., et al. DNA Flow Cytometry Analysis in Body Cavity Fluids Using Liquid-Based Cytology. Diagnostic Cytopathology, (2025), which has been published in final form at https://doi.org/10.1002/dc.25451. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.
抄録	Background: DNA flow cytometry using LC-1000 is utilized for analyzing cellular and nuclear lengths and DNA content in individual cells with samples in cell preservation solutions to acquire the cell proliferation index (CPIx). This study aimed to evaluate the performance of body cavity fluid cytology using LC-1000. As cell preservation solutions, liquid-based cytology (LBC) by Cellprep (CP) was compared with CelVerse (CeV), specifically designed for the LC-1000. Methods: Overall, we evaluated 43 samples (20 malignant and 23 benign) of body cavity fluids suspended in CeV and 59 samples (28 malignant and 31 benign) suspended in CP. The CPIx was compared between malignant and benign cases of CeV and CP. Further, the subject groups were divided according to the SubG1% in a sample. Results: The CPIx in CeV was 0.14–10.22 (median 0.62) in malignant cases and 0.09–4.64 (median 0.19) in benign cases (p < 0.001); additionally, CP displayed 0.14–9.87 (median 0.84) in malignant cases and 0.11–3.02 (median 0.22) in benign cases (p < 0.001). The area under the curve (AUC) was 0.811 for CeV and 0.776 for CP. The difference in SubG1% between the CeV and CP groups was statistically significant (p < 0.0001) and influenced the AUC in CP. Conclusion: The CPIx in CeV or CP samples using the LC-1000 may be applicable as an objective indicator in body cavity fluid cytology. Samples preserved in CeV provided more consistent results, unaffected by SubG1%, when compared to those stored in CP.
出版者	John Wiley and Sons Inc
収録物名	Diagnostic Cytopathology
刊行年月	2025-02-25
言語	英語
ハンドルURL	https://hdl.handle.net/11094/100561
PISSN	87551039
eISSN	10970339
関連情報 (isVersionOf)	DOI (出版社版) https://doi.org/10.1002/dc.25451
アクセス権	embargoed access
権利情報	© 2025 Wiley Periodicals LLC.
権利情報	The full-text file will be made open to the public on 25 February 2026 in accordance with the publisher's policy.
出版タイプ	Accepted Manuscript
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCTERMS.bibliographicCitation	Diagnostic Cytopathology. 2025
DC.title	DNA Flow Cytometry Analysis in Body Cavity Fluids Using Liquid-Based Cytology
DC.creator	Ryu, Ayumi
	Honma, Keiichiro
	Tsuzaki, Sayoko
	Yoshioka, Risa
	Tanada, Satoshi
	Yamamoto, Takashi
	Satomi, Hidetoshi
	Nagata, Shigenori
	Yamasaki, Tomoyuki
	Ohue, Masayuki
DC.publisher	John Wiley and Sons Inc
DC.language' scheme='DCTERMS.RFC1766	英語
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2025-02-25
DC.identifier	https://doi.org/10.1002/dc.25451
DC.subject	body cavity fluids
	DNA
	flow cytometry
	liquid-based cytology
DC.description	This is the peer reviewed version of the following article: Ryu A., Honma K., Tsuzaki S., et al. DNA Flow Cytometry Analysis in Body Cavity Fluids Using Liquid-Based Cytology. Diagnostic Cytopathology, (2025), which has been published in final form at https://doi.org/10.1002/dc.25451. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.
DCTERMS.abstract	Background: DNA flow cytometry using LC-1000 is utilized for analyzing cellular and nuclear lengths and DNA content in individual cells with samples in cell preservation solutions to acquire the cell proliferation index (CPIx). This study aimed to evaluate the performance of body cavity fluid cytology using LC-1000. As cell preservation solutions, liquid-based cytology (LBC) by Cellprep (CP) was compared with CelVerse (CeV), specifically designed for the LC-1000. Methods: Overall, we evaluated 43 samples (20 malignant and 23 benign) of body cavity fluids suspended in CeV and 59 samples (28 malignant and 31 benign) suspended in CP. The CPIx was compared between malignant and benign cases of CeV and CP. Further, the subject groups were divided according to the SubG1% in a sample. Results: The CPIx in CeV was 0.14–10.22 (median 0.62) in malignant cases and 0.09–4.64 (median 0.19) in benign cases (p < 0.001); additionally, CP displayed 0.14–9.87 (median 0.84) in malignant cases and 0.11–3.02 (median 0.22) in benign cases (p < 0.001). The area under the curve (AUC) was 0.811 for CeV and 0.776 for CP. The difference in SubG1% between the CeV and CP groups was statistically significant (p < 0.0001) and influenced the AUC in CP. Conclusion: The CPIx in CeV or CP samples using the LC-1000 may be applicable as an objective indicator in body cavity fluid cytology. Samples preserved in CeV provided more consistent results, unaffected by SubG1%, when compared to those stored in CP.
DC.rights	© 2025 Wiley Periodicals LLC.
DC.rights	The full-text file will be made open to the public on 25 February 2026 in accordance with the publisher's policy.
DC.format	100561
citation_title	DNA Flow Cytometry Analysis in Body Cavity Fluids Using Liquid-Based Cytology
citation_author	Ryu, Ayumi
	Honma, Keiichiro
	Tsuzaki, Sayoko
	Yoshioka, Risa
	Tanada, Satoshi
	Yamamoto, Takashi
	Satomi, Hidetoshi
	Nagata, Shigenori
	Yamasaki, Tomoyuki
	Ohue, Masayuki
citation_publisher	John Wiley and Sons Inc
citation_language	英語
citation_date	2025-02-25
citation_journal_title	Diagnostic Cytopathology
citation_doi	https://doi.org/10.1002/dc.25451
citation_public_url	https://hdl.handle.net/11094/100561

DNA Flow Cytometry Analysis in Body Cavity Fluids Using Liquid-Based Cytology

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