Raman microscopy of cryofixed biological specimens for high-resolution and high-sensitivity chemical imaging

Mizushima, Kenta; Kumamoto, Yasuaki; Tamura, Shoko et al.

Science Advances, 2024, 10(50)

アクセス数:1,277件（2025-08-20 22:36 集計）

固定URL: https://hdl.handle.net/11094/100600

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タイトル
Raman microscopy of cryofixed biological specimens for high-resolution and high-sensitivity chemical imaging
著者
著者名
Mizushima, Kenta
NRID
1000030611727 1000030611727
ORCID
0000-0002-4398-288X 0000-0002-4398-288X
researchmap permalink
jemappellekuma jemappellekuma
Scopus Author ID
24376935000 24376935000
著者名
Kumamoto, Yasuaki
著者名
Tamura, Shoko
NRID
1000090648221 1000090648221
researchmap permalink
masahito_yamanaka masahito_yamanaka
Scopus Author ID
26642849100 26642849100
著者名
Yamanaka, Masahito
NRID
1000050868768 1000050868768
著者名
Mochizuki, Kentaro
著者名
Li, Menglu
NRID
1000060755932 1000060755932
ORCID
0000-0001-7389-9342 0000-0001-7389-9342
researchmap permalink
s.egoshi s.egoshi
著者名
Egoshi, Syusuke
NRID
1000020415243 1000020415243
著者名
Dodo, Kosuke
NRID
1000010381956 1000010381956
著者名
Harada, Yoshinori
著者名
Smith, I. Nicholas
NRID
1000060192142 1000060192142
著者名
Sodeoka, Mikiko
NRID
1000060236619 1000060236619
著者名
Tanaka, Hideo
NRID
1000080362664 1000080362664
ORCID
0000-0002-2284-375X 0000-0002-2284-375X
researchmap permalink
read0089911 read0089911
Scopus Author ID
7404058829 7404058829
著者名
Fujita, Katsumasa
内容
Mizushima K., Kumamoto Y., Tamura S., et al. Raman microscopy of cryofixed biological specimens for high-resolution and high-sensitivity chemical imaging. Science Advances 10, (2024); https://doi.org/10.1126/sciadv.adn0110.
抄録
Raman microscopy is an emerging molecular imaging technology, yet its signal-to- noise ratio (SNR) in measurements of biological specimens is severely limited because of the small cross section of Raman scattering. Here, we present Raman imaging techniques of cryofixed specimens to overcome SNR limitations by enabling long exposure of specimens under highly stabilized low-temperature conditions. The observation of frozen specimens in a cryostat at a constant low temperature immediately after rapid freezing enabled the improvement of SNR and enhanced the spatial and spectral resolution. We also confirmed that the cryofixation can preserve physicochemical states of specimens by observing alkyne-labeled coenzyme Q in cytosol and hemeproteins in acute ischemic myocardium, which cannot be done by fixation using chemical reagents. Last, we applied the technique for multiplex Raman imaging of label-free endogenous molecules and alkyne-tagged molecules in cryofixed HeLa cells, demonstrating its capability of high-content imaging of complex biological phenomena while maintaining physiological conditions.
出版者
American Association for the Advancement of Science
収録物名
Science Advances
巻(号)
10(50)
刊行年月
2024-12-13
言語
英語
ハンドルURL
https://hdl.handle.net/11094/100600
eISSN
23752548
関連情報 (isIdenticalTo)
DOI (出版社版)
https://doi.org/10.1126/sciadv.adn0110
アクセス権
open access
権利情報
This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ
Version of Record
カテゴリ
学術雑誌論文 Journal Article