Effects of moderate static magnetic fields on voltage-gated potassium ion channels in sympathetic neuron-like PC12 cells

Kaneda, Eri; Kawai, Takafumi; Okamura, Yasushi; Miyagawa, Shigeru

doi:https://doi.org/10.14814/phy2.70236

Effects of moderate static magnetic fields on voltage-gated potassium ion channels in sympathetic neuron-like PC12 cells

Kaneda, Eri; Kawai, Takafumi; Okamura, Yasushi et al.

Physiological Reports, 2025, 13(6), e70236

アクセス数:568件（2025-07-22 20:55 集計）

固定URL: https://hdl.handle.net/11094/100966

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タイトル	Effects of moderate static magnetic fields on voltage-gated potassium ion channels in sympathetic neuron-like PC12 cells
著者	著者名 Kaneda, Eri
	NRID 1000070614915 1000070614915 researchmap permalink t_kawa t_kawa Scopus Author ID 55465095900 55465095900 著者名 Kawai, Takafumi
	NRID 1000080201987 1000080201987 ORCID 0000-0001-5386-7968 0000-0001-5386-7968 researchmap permalink yasushiokamura yasushiokamura Scopus Author ID 55322083200 55322083200 著者名 Okamura, Yasushi
	NRID 1000070544237 1000070544237 ORCID 0000-0003-0015-6569 0000-0003-0015-6569 researchmap permalink s-miyagawa3154 s-miyagawa3154 Scopus Author ID 55560624700 55560624700 著者名 Miyagawa, Shigeru
キーワード等	neuromodulation
	patch clamp
	static magnetic fields
	sympathetic neuron
内容	Kaneda E., Kawai T., Okamura Y., et al. Effects of moderate static magnetic fields on voltage-gated potassium ion channels in sympathetic neuron-like PC12 cells. Physiological Reports 13, e70236 (2025); https://doi.org/10.14814/phy2.70236.
抄録	While exposure of moderate static magnetic fields (SMF) can alter neuronal excitability, the effects on sympathetic neurons remain underexplored. This study investigates the effects of moderate SMF on Kv channels in the plasma membrane of sympathetic neuron-like PC12 cells. The current density of Kv channels was significantly lower in the 18-h magnet-exposed group, with effects persisting even after the magnet was removed before patch-clamp measurements. The current density of outward current in the presence of TEA was not different between the two groups, indicating that magnetic field affects TEA-sensitive Kv channels. To further explore these changes, RNA sequencing was performed on samples from both the Sham and 18-h magnet-exposed groups, identifying 37 moderate SMF-sensitive genes. Changes in mRNA expression levels and KEGG analysis suggested that pathways involved in the inhibition of neuronal excitability, such as GABAB receptor activation and Kir3 channel opening, may be more likely to be activated. In conclusion, moderate SMF is strongly associated with reduced current density in PC12 cells, particularly affecting Kv channels. The present study provides fundamental information on the influence of long-term SMF exposure on the excitability of sympathetic neurons.
出版者	American Physiological Society
収録物名	Physiological Reports
巻(号)	13(6)
ページ	e70236
刊行年月	2025-03-22
言語	英語
ハンドルURL	https://hdl.handle.net/11094/100966
eISSN	2051817X
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.14814/phy2.70236
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCTERMS.bibliographicCitation	Physiological Reports. 2025, 13(6), p. e70236
DC.title	Effects of moderate static magnetic fields on voltage-gated potassium ion channels in sympathetic neuron-like PC12 cells
DC.creator	Kaneda, Eri
	Kawai, Takafumi
	Okamura, Yasushi
	Miyagawa, Shigeru
DC.publisher	American Physiological Society
DC.language' scheme='DCTERMS.RFC1766	英語
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2025-03-22
DC.identifier	https://doi.org/10.14814/phy2.70236
DC.subject	neuromodulation
	patch clamp
	static magnetic fields
	sympathetic neuron
DC.description	Kaneda E., Kawai T., Okamura Y., et al. Effects of moderate static magnetic fields on voltage-gated potassium ion channels in sympathetic neuron-like PC12 cells. Physiological Reports 13, e70236 (2025); https://doi.org/10.14814/phy2.70236.
DCTERMS.abstract	While exposure of moderate static magnetic fields (SMF) can alter neuronal excitability, the effects on sympathetic neurons remain underexplored. This study investigates the effects of moderate SMF on Kv channels in the plasma membrane of sympathetic neuron-like PC12 cells. The current density of Kv channels was significantly lower in the 18-h magnet-exposed group, with effects persisting even after the magnet was removed before patch-clamp measurements. The current density of outward current in the presence of TEA was not different between the two groups, indicating that magnetic field affects TEA-sensitive Kv channels. To further explore these changes, RNA sequencing was performed on samples from both the Sham and 18-h magnet-exposed groups, identifying 37 moderate SMF-sensitive genes. Changes in mRNA expression levels and KEGG analysis suggested that pathways involved in the inhibition of neuronal excitability, such as GABAB receptor activation and Kir3 channel opening, may be more likely to be activated. In conclusion, moderate SMF is strongly associated with reduced current density in PC12 cells, particularly affecting Kv channels. The present study provides fundamental information on the influence of long-term SMF exposure on the excitability of sympathetic neurons.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	100966
citation_title	Effects of moderate static magnetic fields on voltage-gated potassium ion channels in sympathetic neuron-like PC12 cells
citation_author	Kaneda, Eri
	Kawai, Takafumi
	Okamura, Yasushi
	Miyagawa, Shigeru
citation_publisher	American Physiological Society
citation_language	英語
citation_date	2025-03-22
citation_journal_title	Physiological Reports
citation_volume	13
citation_issue	6
citation_firstpage	e70236
citation_doi	https://doi.org/10.14814/phy2.70236
citation_public_url	https://hdl.handle.net/11094/100966

Effects of moderate static magnetic fields on voltage-gated potassium ion channels in sympathetic neuron-like PC12 cells

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