Correlation between the metabolic profile of Nelumbo Seed, a component of Seishinrenshiin, and its inhibitory activity on bladder smooth muscle contraction

Harada, Kazuo; Fukuda, Yuki; Ohkubo, Takahiro; Sugaya, Kimio; Osaki, Yukihiko

doi:https://doi.org/10.1007/s11418-025-01889-4

Correlation between the metabolic profile of Nelumbo Seed, a component of Seishinrenshiin, and its inhibitory activity on bladder smooth muscle contraction

Harada, Kazuo; Fukuda, Yuki; Ohkubo, Takahiro et al.

Journal of Natural Medicines, 2025

アクセス数:334件（2025-11-23 10:22 集計）

固定URL: https://hdl.handle.net/11094/101008

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タイトル	Correlation between the metabolic profile of Nelumbo Seed, a component of Seishinrenshiin, and its inhibitory activity on bladder smooth muscle contraction
著者	NRID 1000050397741 1000050397741 ORCID 0000-0002-9348-1629 0000-0002-9348-1629 researchmap permalink read0204933 read0204933 Scopus Author ID 16549528100 16549528100 著者名 Harada, Kazuo
	著者名 Fukuda, Yuki
	著者名 Ohkubo, Takahiro
	NRID 1000020179120 1000020179120 著者名 Sugaya, Kimio
	著者名 Osaki, Yukihiko
キーワード等	Bladder smooth muscle
	Metabolic profiling
	Neferine
	Nelumbo Seed
	Seishinrenshiin
内容	The version of record of this article, first published in Journal of Natural Medicines, is available online at Publisher’s website: https://doi.org/10.1007/s11418-025-01889-4
抄録	Seishinrenshiin (SRI), a Kampo formula, is often used for frequent urination. Nelumbo Seed, a crude-drug component of SRI was reported to inhibit bladder smooth muscle contraction using excised rat bladder tissues. However, the active ingredients of Nelumbo Seed have not yet been identified. In this study, we investigated the active ingredients of Nelumbo Seed that inhibit bladder smooth muscle contraction. We obtained liquid chromatography/mass spectrometry profiles of extracts prepared from five types of Nelumbo Seed materials from different production areas and evaluated their inhibitory effects on excised rat bladder smooth muscle contraction. Analysis of these data using orthogonal projections to latent structures revealed neferine (Nef) as the compound with the highest variable influence on projection. Among the Nelumbo Seed materials processed using different methods, the Nef content was the highest in ‘Sekirenshi’, when embryo and pericarp were not removed at all. We determined the effects of administering Nelumbo Seed extracts with different Nef content on frequent-urination model rats. The Nef content in rat plasma was the highest when the Sekirenshi extract was administered, but Nef was not detected when seed embryos were removed. In this model, administration of the Nelumbo Seed extract improved the maximum bladder contraction pressure and bladder contraction interval, albeit not significantly. In particular, Sekirenshi tended to reduce the maximum bladder contraction pressure compared to other Nelumbo Seed. Our results indicate that although Nef in Nelumbo Seed does not clearly improve frequent urination, it might contribute to the improvement of urination disorder.
出版者	Springer
収録物名	Journal of Natural Medicines
刊行年月	2025-03-19
言語	英語
ハンドルURL	https://hdl.handle.net/11094/101008
PISSN	13403443
eISSN	18610293
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1007/s11418-025-01889-4
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCTERMS.bibliographicCitation	Journal of Natural Medicines. 2025
DC.title	Correlation between the metabolic profile of Nelumbo Seed, a component of Seishinrenshiin, and its inhibitory activity on bladder smooth muscle contraction
DC.creator	Harada, Kazuo
	Fukuda, Yuki
	Ohkubo, Takahiro
	Sugaya, Kimio
	Osaki, Yukihiko
DC.publisher	Springer
DC.language' scheme='DCTERMS.RFC1766	英語
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2025-03-19
DC.identifier	https://doi.org/10.1007/s11418-025-01889-4
DC.subject	Bladder smooth muscle
	Metabolic profiling
	Neferine
	Nelumbo Seed
	Seishinrenshiin
DC.description	The version of record of this article, first published in Journal of Natural Medicines, is available online at Publisher’s website: https://doi.org/10.1007/s11418-025-01889-4
DCTERMS.abstract	Seishinrenshiin (SRI), a Kampo formula, is often used for frequent urination. Nelumbo Seed, a crude-drug component of SRI was reported to inhibit bladder smooth muscle contraction using excised rat bladder tissues. However, the active ingredients of Nelumbo Seed have not yet been identified. In this study, we investigated the active ingredients of Nelumbo Seed that inhibit bladder smooth muscle contraction. We obtained liquid chromatography/mass spectrometry profiles of extracts prepared from five types of Nelumbo Seed materials from different production areas and evaluated their inhibitory effects on excised rat bladder smooth muscle contraction. Analysis of these data using orthogonal projections to latent structures revealed neferine (Nef) as the compound with the highest variable influence on projection. Among the Nelumbo Seed materials processed using different methods, the Nef content was the highest in ‘Sekirenshi’, when embryo and pericarp were not removed at all. We determined the effects of administering Nelumbo Seed extracts with different Nef content on frequent-urination model rats. The Nef content in rat plasma was the highest when the Sekirenshi extract was administered, but Nef was not detected when seed embryos were removed. In this model, administration of the Nelumbo Seed extract improved the maximum bladder contraction pressure and bladder contraction interval, albeit not significantly. In particular, Sekirenshi tended to reduce the maximum bladder contraction pressure compared to other Nelumbo Seed. Our results indicate that although Nef in Nelumbo Seed does not clearly improve frequent urination, it might contribute to the improvement of urination disorder.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	101008
citation_title	Correlation between the metabolic profile of Nelumbo Seed, a component of Seishinrenshiin, and its inhibitory activity on bladder smooth muscle contraction
citation_author	Harada, Kazuo
	Fukuda, Yuki
	Ohkubo, Takahiro
	Sugaya, Kimio
	Osaki, Yukihiko
citation_publisher	Springer
citation_language	英語
citation_date	2025-03-19
citation_journal_title	Journal of Natural Medicines
citation_doi	https://doi.org/10.1007/s11418-025-01889-4
citation_public_url	https://hdl.handle.net/11094/101008

Correlation between the metabolic profile of Nelumbo Seed, a component of Seishinrenshiin, and its inhibitory activity on bladder smooth muscle contraction

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