The H3K9 demethylase plant homeodomain finger protein 2 regulates interleukin 4 production in CD4+ T cells

Arakawa, Yuya; Tano, Yuzuki; Fujii, Moe; Imai, Yuuki; Norimatsu, Yoshiaki; Yasukawa, Masaki; Watanabe, Mikio; Yamada, Takeshi

doi:https://doi.org/10.1016/j.cyto.2024.156506

The H3K9 demethylase plant homeodomain finger protein 2 regulates interleukin 4 production in CD4+ T cells

Arakawa, Yuya; Tano, Yuzuki; Fujii, Moe et al.

Cytokine, 2024, 175, 156506

アクセス数:711件（2025-08-16 19:03 集計）

固定URL: https://hdl.handle.net/11094/101098

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タイトル	The H3K9 demethylase plant homeodomain finger protein 2 regulates interleukin 4 production in CD4+ T cells
著者	NRID 1000030733175 1000030733175 researchmap permalink ara-y ara-y 著者名 Arakawa, Yuya
	NRID 1000090983809 1000090983809 著者名 Tano, Yuzuki
	著者名 Fujii, Moe
	NRID 1000010423873 1000010423873 著者名 Imai, Yuuki
	NRID 1000090511189 1000090511189 著者名 Norimatsu, Yoshiaki
	NRID 1000060127917 1000060127917 著者名 Yasukawa, Masaki
	NRID 1000050294088 1000050294088 ORCID 0000-0001-5971-9296 0000-0001-5971-9296 researchmap permalink watanabemikio watanabemikio Scopus Author ID 55793188612 55793188612 著者名 Watanabe, Mikio
	NRID 1000040333554 1000040333554 著者名 Yamada, Takeshi
キーワード等	CD4+ T cells
	Histone methylation
	Interferon-gamma
	Interleukin 4
	Plant homeodomain finger protein 2
	T helper 2 cells
抄録	CD4+ T cells play a key role in the immune response via their differentiation into various helper T cell subsets that produce characteristic cytokines. Epigenetic changes in CD4+ T cells are responsible for cytokine production in these subsets, although the exact molecular mechanisms remain unclear. Therefore, we investigated the effects of plant homeodomain finger protein 2 (PHF2), a histone H3K9 demethylase, on cytokine production in CD4+ T cells using T cell-specific Phf2-conditional knockout (cKO) mice in this study. we showed that interleukin 4 (Il4) expression was significantly decreased in Phf2-cKO CD4+ T cells compared to that in wild-type cells. To further elucidate the role of PHF2 in vivo, we assessed immune responses in a mouse model of ovalbumin (OVA)-induced atopic dermatitis. Phf2-cKO mice exhibited lower serum levels of OVA-specific IgE than those in wild-type mice. These findings suggest that PHF2 plays a role in promoting T helper 2 cell (Th2) function and may contribute to the pathogenesis of Th2-related allergies such as atopic dermatitis. This study demonstrated the impact of PHF2 on cytokine production in CD4+ T cells for the first time. Further studies on the PHF2-mediated epigenetic mechanisms may lead to the development of treatments for a variety of immune diseases.
出版者	Academic Press
収録物名	Cytokine
巻(号)	175
ページ	156506
刊行年月	2024-03-01
言語	英語
ハンドルURL	https://hdl.handle.net/11094/101098
PISSN	10434666
eISSN	10960023
関連情報 (isVersionOf)	DOI (出版社版) https://doi.org/10.1016/j.cyto.2024.156506
関連情報 (isSupplementedBy)	HDL https://hdl.handle.net/11094/101099
アクセス権	open access
権利情報	© 2024. This manuscript version is made available under the CC-BY-NC-ND 4.0 license.
出版タイプ	Accepted Manuscript
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Cytokine. 2024, 175, p. 156506
DC.title	The H3K9 demethylase plant homeodomain finger protein 2 regulates interleukin 4 production in CD4+ T cells
DC.creator	Arakawa, Yuya
	Tano, Yuzuki
	Fujii, Moe
	Imai, Yuuki
	Norimatsu, Yoshiaki
	Yasukawa, Masaki
	Watanabe, Mikio
	Yamada, Takeshi
DC.publisher	Academic Press
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2024-03-01
DC.identifier	https://doi.org/10.1016/j.cyto.2024.156506
DC.identifier' scheme='DCTERMS.URI	https://hdl.handle.net/11094/101099
DC.subject	CD4+ T cells
	Histone methylation
	Interferon-gamma
	Interleukin 4
	Plant homeodomain finger protein 2
	T helper 2 cells
DCTERMS.abstract	CD4+ T cells play a key role in the immune response via their differentiation into various helper T cell subsets that produce characteristic cytokines. Epigenetic changes in CD4+ T cells are responsible for cytokine production in these subsets, although the exact molecular mechanisms remain unclear. Therefore, we investigated the effects of plant homeodomain finger protein 2 (PHF2), a histone H3K9 demethylase, on cytokine production in CD4+ T cells using T cell-specific Phf2-conditional knockout (cKO) mice in this study. we showed that interleukin 4 (Il4) expression was significantly decreased in Phf2-cKO CD4+ T cells compared to that in wild-type cells. To further elucidate the role of PHF2 in vivo, we assessed immune responses in a mouse model of ovalbumin (OVA)-induced atopic dermatitis. Phf2-cKO mice exhibited lower serum levels of OVA-specific IgE than those in wild-type mice. These findings suggest that PHF2 plays a role in promoting T helper 2 cell (Th2) function and may contribute to the pathogenesis of Th2-related allergies such as atopic dermatitis. This study demonstrated the impact of PHF2 on cytokine production in CD4+ T cells for the first time. Further studies on the PHF2-mediated epigenetic mechanisms may lead to the development of treatments for a variety of immune diseases.
DC.rights	© 2024. This manuscript version is made available under the CC-BY-NC-ND 4.0 license.
DC.format	101098
citation_title	The H3K9 demethylase plant homeodomain finger protein 2 regulates interleukin 4 production in CD4+ T cells
citation_author	Arakawa, Yuya
	Tano, Yuzuki
	Fujii, Moe
	Imai, Yuuki
	Norimatsu, Yoshiaki
	Yasukawa, Masaki
	Watanabe, Mikio
	Yamada, Takeshi
citation_publisher	Academic Press
citation_language	英語
citation_date	2024-03-01
citation_journal_title	Cytokine
citation_volume	175
citation_firstpage	156506
citation_doi	https://doi.org/10.1016/j.cyto.2024.156506
citation_public_url	https://hdl.handle.net/11094/101098

The H3K9 demethylase plant homeodomain finger protein 2 regulates interleukin 4 production in CD4+ T cells

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