Oridonin Suppresses Mast Cell Degranulation and Alleviates Ovalbumin-Induced Allergic Rhinitis

Kidawara, Sota; Kimura, Shizuki; Takano, Haruka; Inoue, Wakana; Iinuma, Kota; Takahama, Michihiro; Takemura, Naoki; Saitoh, Tatsuya

doi:https://doi.org/10.1248/bpb.b24-00594

Oridonin Suppresses Mast Cell Degranulation and Alleviates Ovalbumin-Induced Allergic Rhinitis

Kidawara, Sota; Kimura, Shizuki; Takano, Haruka et al.

Biological and Pharmaceutical Bulletin, 2024, 47(11), 1920-1926

アクセス数:356件（2025-07-31 20:23 集計）

固定URL: https://hdl.handle.net/11094/101357

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タイトル	Oridonin Suppresses Mast Cell Degranulation and Alleviates Ovalbumin-Induced Allergic Rhinitis
著者	著者名 Kidawara, Sota
	著者名 Kimura, Shizuki
	著者名 Takano, Haruka
	著者名 Inoue, Wakana
	著者名 Iinuma, Kota
	NRID 1000010774755 1000010774755 researchmap permalink mtakahama mtakahama 著者名 Takahama, Michihiro
	NRID 1000050648699 1000050648699 researchmap permalink ntakemura ntakemura Scopus Author ID 57190744932 57190744932 著者名 Takemura, Naoki
	NRID 1000060456936 1000060456936 researchmap permalink saitohtatsuya saitohtatsuya Scopus Author ID 7401688725 7401688725 著者名 Saitoh, Tatsuya
キーワード等	allergic rhinitis
	degranulation
	mast cell
	oridonin
内容	Kidawara S., Kimura S., Takano H., et al. Oridonin Suppresses Mast Cell Degranulation and Alleviates Ovalbumin-Induced Allergic Rhinitis. Biological and Pharmaceutical Bulletin 47, 1920 (2024); https://doi.org/10.1248/bpb.b24-00594.
抄録	Incidence of type I allergies, such as hay fever, is continuously increasing in developed countries, including Japan. Type I allergies are triggered by chemical mediators, such as histamine, which are released via immunoglobulin E (IgE)-mediated mast cell degranulation. Therefore, medications inhibiting the synthesis, release, and receptor binding of these mediators are commonly used to manage type I allergy symptoms. As self-care disease prevention practices are gaining attention worldwide, regular consumption of food and supplements containing safe components inhibiting mast cell degranulation is a potential strategy to prevent allergic attacks. Here, we aimed to assess the ability of phytochemicals derived from edible plants to inhibit mast cell degranulation using the β-hexosaminidase release assay and investigate their cytotoxicity and efficiency in alleviating allergic symptoms. We found that oridonin, a diterpenoid isolated from Isodon japonicus Hara, strongly inhibited β-hexosaminidase release from both the RBL-2H3 rat cell line and mouse bone marrow-derived mast cells stimulated with dinitrophenyl (DNP)-conjugated human serum albumin after sensitization with DNP-IgE. Oridonin also inhibited β-hexosaminidase release induced by the calcium ionophore, A23187, in both cell types. Notably, oridonin did not adversely affect cell survival at concentrations necessary to inhibit β-hexosaminidase release. In a mouse model of ovalbumin (OVA)-induced allergic rhinitis, intraperitoneal administration of oridonin significantly reduced the nasal rubbing caused by intranasal OVA administration without affecting the serum levels of OVA-specific IgE. Therefore, oridonin could be an effective daily intake component to alleviate allergic diseases by inhibiting mast cell degranulation.
出版者	Pharmaceutical Society of Japan
収録物名	Biological and Pharmaceutical Bulletin
巻(号)	47(11)
ページ	1920-1926
刊行年月	2024-11-01
言語	英語
ハンドルURL	https://hdl.handle.net/11094/101357
PISSN	09186158
eISSN	13475215
関連情報 (isSupplementedBy)	DOI (出版社版) https://doi.org/10.60574/101358
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1248/bpb.b24-00594
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Biological and Pharmaceutical Bulletin. 2024, 47(11), p. 1920-1926
DC.title	Oridonin Suppresses Mast Cell Degranulation and Alleviates Ovalbumin-Induced Allergic Rhinitis
DC.creator	Kidawara, Sota
	Kimura, Shizuki
	Takano, Haruka
	Inoue, Wakana
	Iinuma, Kota
	Takahama, Michihiro
	Takemura, Naoki
	Saitoh, Tatsuya
DC.publisher	Pharmaceutical Society of Japan
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2024-11-01
DC.identifier	https://doi.org/10.1248/bpb.b24-00594
DC.identifier	https://doi.org/10.60574/101358
DC.subject	allergic rhinitis
	degranulation
	mast cell
	oridonin
DC.description	Kidawara S., Kimura S., Takano H., et al. Oridonin Suppresses Mast Cell Degranulation and Alleviates Ovalbumin-Induced Allergic Rhinitis. Biological and Pharmaceutical Bulletin 47, 1920 (2024); https://doi.org/10.1248/bpb.b24-00594.
DCTERMS.abstract	Incidence of type I allergies, such as hay fever, is continuously increasing in developed countries, including Japan. Type I allergies are triggered by chemical mediators, such as histamine, which are released via immunoglobulin E (IgE)-mediated mast cell degranulation. Therefore, medications inhibiting the synthesis, release, and receptor binding of these mediators are commonly used to manage type I allergy symptoms. As self-care disease prevention practices are gaining attention worldwide, regular consumption of food and supplements containing safe components inhibiting mast cell degranulation is a potential strategy to prevent allergic attacks. Here, we aimed to assess the ability of phytochemicals derived from edible plants to inhibit mast cell degranulation using the β-hexosaminidase release assay and investigate their cytotoxicity and efficiency in alleviating allergic symptoms. We found that oridonin, a diterpenoid isolated from Isodon japonicus Hara, strongly inhibited β-hexosaminidase release from both the RBL-2H3 rat cell line and mouse bone marrow-derived mast cells stimulated with dinitrophenyl (DNP)-conjugated human serum albumin after sensitization with DNP-IgE. Oridonin also inhibited β-hexosaminidase release induced by the calcium ionophore, A23187, in both cell types. Notably, oridonin did not adversely affect cell survival at concentrations necessary to inhibit β-hexosaminidase release. In a mouse model of ovalbumin (OVA)-induced allergic rhinitis, intraperitoneal administration of oridonin significantly reduced the nasal rubbing caused by intranasal OVA administration without affecting the serum levels of OVA-specific IgE. Therefore, oridonin could be an effective daily intake component to alleviate allergic diseases by inhibiting mast cell degranulation.
DC.rights	This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
DC.format	101357
citation_title	Oridonin Suppresses Mast Cell Degranulation and Alleviates Ovalbumin-Induced Allergic Rhinitis
citation_author	Kidawara, Sota
	Kimura, Shizuki
	Takano, Haruka
	Inoue, Wakana
	Iinuma, Kota
	Takahama, Michihiro
	Takemura, Naoki
	Saitoh, Tatsuya
citation_publisher	Pharmaceutical Society of Japan
citation_language	英語
citation_date	2024-11-01
citation_journal_title	Biological and Pharmaceutical Bulletin
citation_volume	47
citation_issue	11
citation_firstpage	1920
citation_lastpage	1926
citation_doi	https://doi.org/10.1248/bpb.b24-00594
citation_doi	https://doi.org/10.60574/101358
citation_public_url	https://hdl.handle.net/11094/101357

Oridonin Suppresses Mast Cell Degranulation and Alleviates Ovalbumin-Induced Allergic Rhinitis

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