学術雑誌論文

学術雑誌論文

text

Protein Science. 2025, 34, p. e70132

De novo design of ATPase based on a blueprint optimized for harboring the P-loop motif

Wiley

英語

text

2025-05-13

https://doi.org/10.1002/pro.70132

Kosugi Takahiro, Tanabe Mikio, Koga Nobuyasu. De novo design of ATPase based on a blueprint optimized for harboring the P-loop motif. Protein Science 34, e70132 (2025); https://doi.org/10.1002/pro.70132.

De novo design of proteins has seen remarkable recent progress and has provided understanding of folding and functional expression. However, rationally creating enzymes with high activity comparable to most naturally occurring enzymes remains challenging. Here, we attempted to design an ATPase de novo, through the exploration of an optimal backbone blueprint to incorporate a conserved phosphate binding motif, the P-loop, into designed structures. The designed protein, based on the identified blueprint, was found to be a monomer with high thermal stability and exhibited ATPase ability. The crystal structure was closely matched to the design model, both at the overall structure level and within the P-loop motif. Interestingly, AlphaFold 2 was not able to predict the designed structure accurately, indicating the difficulties of predicting folded structures for novel amino acid sequences. Remarkably, the designed protein exhibited ATPase ability even at temperatures around 100°C, with significantly increased activity. However, the ATPase activity was still not comparable to those of naturally occurring enzymes. This suggests that the P-loop motif alone is insufficient to achieve the high ATPase activity seen in naturally occurring enzymes, indicating that other structural components—such as a binding pocket optimized for the adenine or ribose moieties of ATP, additional catalytic residues, or structural dynamics that facilitate hydrolysis—are necessary to reach such activity levels.

This article is licensed under a Creative Commons Attribution 4.0 International License.

101406

De novo design of ATPase based on a blueprint optimized for harboring the P-loop motif

Wiley

英語

2025-05-13

Protein Science

34

e70132

https://doi.org/10.1002/pro.70132

https://hdl.handle.net/11094/101406