Regulation of Kinetic Properties of GluR2 AMPA Receptor Channels by Alternative Splicing

Koike, Maki; Tsukada, Shota; Tsuzuki, Keisuke et al.

Journal of Neuroscience, 2000, 20(6), 2166-2174

アクセス数:24件（2025-08-15 23:01 集計）

固定URL: https://hdl.handle.net/11094/101969

閲覧可能ファイル 

ファイル フォーマット 利用条件 サイズ 閲覧回数 利用開始日 説明 information
JNeurosci_20_6_2166 pdf なし 167 KB 15 2025.06.20  

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

タイトル
Regulation of Kinetic Properties of GluR2 AMPA Receptor Channels by Alternative Splicing
著者
NRID
1000060396702 1000060396702
ORCID
0000-0003-2679-5975 0000-0003-2679-5975
researchmap permalink
mktani mktani
著者名
Koike, Maki
著者名
Tsukada, Shota
著者名
Tsuzuki, Keisuke
NRID
1000030012397 1000030012397
著者名
Kijima, Hiromasa
著者名
Ozawa, Seiji
キーワード等
AMPA receptor channel
GluR2
Flip/flop splice variants
Q/R site
Desensitization
Deactivation
内容
Koike M., Tsukada S., Tsuzuki K., et al. Regulation of kinetic properties of GluR2 AMPA receptor channels by alternative splicing. Journal of Neuroscience 20, 2166 (2000); https://doi.org/10.1523/JNEUROSCI.20-06-02166.2000.
抄録
The four subunits of the AMPA-type glutamate receptor (GluR1-GluR4 or GluR-A-GluR-D) exist in two distinct forms, flip and flop, generated by alternative splicing of a 115 bp region. The GluR2 subunit plays a key role in determining the functional properties of the AMPA receptor channel. In this study, we examined the differences in kinetic properties between the flip and flop splice variants of the GluR2 subunit expressed in Xenopus oocytes using fast agonist application techniques. Glutamate was applied to outside-out patches from oocytes with piezo-driven double-barreled application pipettes. Because homomeric receptor channels composed of the edited form of GluR2 (GluR2R) produce no appreciable current responses, we expressed the unedited form of GluR2 (GluR2Q) in oocytes, which produced large current responses sufficient for analysis of the kinetic properties. The time constant for desensitization during application of 1 mM glutamate was 5.89 ± 0.17 msec (n = 50) in flip and 1.18 ± 0.05 msec (n = 37) in flop. The deactivation time constant was 0.62 ± 0.06 msec (n = 10) in flip and 0.54 ± 0.05 msec (n = 10) in flop. The steady-state nondesensitizing current was 6.8 ± 0.4% (n = 53) of the peak current in flip, whereas it was almost negligible in flop, being only 1.1 ± 0.1% (n = 36). The slower desensitization kinetics and larger steady-state current responses in the flip variant were also observed in heteromeric receptors assembled from GluR2Q/GluR2R. Thus, desensitization occurred much more prominently in the flop variant in the recombinant GluR2 receptor channels.
出版者
Society for Neuroscience
収録物名
Journal of Neuroscience
巻(号)
20(6)
ページ
2166-2174
刊行年月
2000-03-15
言語
英語
ハンドルURL
https://hdl.handle.net/11094/101969
PISSN
02706474
関連情報 (isIdenticalTo)
DOI (出版社版)
https://doi.org/10.1523/JNEUROSCI.20-06-02166.2000
アクセス権
open access
出版タイプ
Version of Record
カテゴリ
学術雑誌論文 Journal Article