Tiny but mighty! N,N-dimethyl-4-(5-nitrothiophen-2-yl)aniline, a push-pull fluorescent dye for lipid droplet imaging

Wee, Wen Ann; Andini, Tatum Melati; Kumagai, Tomotaka; Chinnathambi, Shanmugavel; Pandian, Ganesh N.; Millius, Arthur; Sugiyama, Hiroshi; Standley, Daron M.; Park, Soyoung

doi:https://doi.org/10.1016/j.aca.2025.344130

Tiny but mighty! N,N-dimethyl-4-(5-nitrothiophen-2-yl)aniline, a push-pull fluorescent dye for lipid droplet imaging

Wee, Wen Ann; Andini, Tatum Melati; Kumagai, Tomotaka et al.

Analytica Chimica Acta, 2025, 1359, 344130

アクセス数:14件（2025-08-20 14:19 集計）

固定URL: https://hdl.handle.net/11094/102199

閲覧可能ファイル

ファイル	フォーマット	利用条件	サイズ	閲覧回数	利用開始日	説明	information
AnalChimActa_1359	pdf	なし	6.84 MB	31	2025.07.10

Creative Commons : 表示 - 非営利

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

タイトル	Tiny but mighty! N,N-dimethyl-4-(5-nitrothiophen-2-yl)aniline, a push-pull fluorescent dye for lipid droplet imaging
著者	著者名 Wee, Wen Ann
	著者名 Andini, Tatum Melati
	著者名 Kumagai, Tomotaka
	NRID 1000060922341 1000060922341 著者名 Chinnathambi, Shanmugavel
	著者名 Pandian, Ganesh N.
	NRID 1000080624858 1000080624858 著者名 Millius, Arthur
	著者名 Sugiyama, Hiroshi
	NRID 1000000448028 1000000448028 researchmap permalink 7000011861 7000011861 著者名 Standley, Daron M.
	NRID 1000010628556 1000010628556 researchmap permalink 7000009174 7000009174 著者名 Park, Soyoung
キーワード等	Fluorescent probe
	LD probe
	Lipid droplets
	Push-pull dye
	Solvatochromism
内容	Wee W.A., Andini T.M., Kumagai T., et al. Tiny but mighty! N,N-dimethyl-4-(5-nitrothiophen-2-yl)aniline, a push-pull fluorescent dye for lipid droplet imaging. Analytica Chimica Acta 1359, 344130 (2025); https://doi.org/10.1016/j.aca.2025.344130.
抄録	Lipid droplets (LDs) are ubiquitous cellular organelles with a neutral lipid core containing triacylglycerols and cholesteryl esters surrounded by phospholipids. Recent findings indicate that LDs are intricately linked to diseases, such as cancer and neurological disorders, in addition to their roles in cellular senescence and immune responses. Herein, we describe a simple yet robust push-pull molecule, N,N-dimethyl-4-(5-nitrothiophen-2-yl)aniline (NiTA), as a versatile LD fluorescent probe. NiTA showed an absorption spectrum with a substantial bathochromic shift and a fluorescence spectrum with excellent solvatochromism. Leveraging the remarkable photophysical features of NiTA, we stained LDs in major immune cells, including T and B cells, and macrophages, and monitored the changes in LDs under oxidative and starvation conditions. Furthermore, we demonstrated the applicability of NiTA for visualizing the organization of medaka fish (Oryzias latipes) embryos during development. We expect the small yet powerful NiTA to be utilized in various applications, including fluorescence mapping to observe LD numbers, morphology, and polarity changes in animals and cells.
出版者	Elsevier B.V.
収録物名	Analytica Chimica Acta
巻(号)	1359
ページ	344130
刊行年月	2025-05-03
言語	英語
ハンドルURL	https://hdl.handle.net/11094/102199
PISSN	00032670
eISSN	18734324
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1016/j.aca.2025.344130
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Analytica Chimica Acta. 2025, 1359, p. 344130
DC.title	Tiny but mighty! N,N-dimethyl-4-(5-nitrothiophen-2-yl)aniline, a push-pull fluorescent dye for lipid droplet imaging
DC.creator	Wee, Wen Ann
	Andini, Tatum Melati
	Kumagai, Tomotaka
	Chinnathambi, Shanmugavel
	Pandian, Ganesh N.
	Millius, Arthur
	Sugiyama, Hiroshi
	Standley, Daron M.
	Park, Soyoung
DC.publisher	Elsevier B.V.
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2025-05-03
DC.identifier	https://doi.org/10.1016/j.aca.2025.344130
DC.subject	Fluorescent probe
	LD probe
	Lipid droplets
	Push-pull dye
	Solvatochromism
DC.description	Wee W.A., Andini T.M., Kumagai T., et al. Tiny but mighty! N,N-dimethyl-4-(5-nitrothiophen-2-yl)aniline, a push-pull fluorescent dye for lipid droplet imaging. Analytica Chimica Acta 1359, 344130 (2025); https://doi.org/10.1016/j.aca.2025.344130.
DCTERMS.abstract	Lipid droplets (LDs) are ubiquitous cellular organelles with a neutral lipid core containing triacylglycerols and cholesteryl esters surrounded by phospholipids. Recent findings indicate that LDs are intricately linked to diseases, such as cancer and neurological disorders, in addition to their roles in cellular senescence and immune responses. Herein, we describe a simple yet robust push-pull molecule, N,N-dimethyl-4-(5-nitrothiophen-2-yl)aniline (NiTA), as a versatile LD fluorescent probe. NiTA showed an absorption spectrum with a substantial bathochromic shift and a fluorescence spectrum with excellent solvatochromism. Leveraging the remarkable photophysical features of NiTA, we stained LDs in major immune cells, including T and B cells, and macrophages, and monitored the changes in LDs under oxidative and starvation conditions. Furthermore, we demonstrated the applicability of NiTA for visualizing the organization of medaka fish (Oryzias latipes) embryos during development. We expect the small yet powerful NiTA to be utilized in various applications, including fluorescence mapping to observe LD numbers, morphology, and polarity changes in animals and cells.
DC.rights	This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
DC.format	102199
citation_title	Tiny but mighty! N,N-dimethyl-4-(5-nitrothiophen-2-yl)aniline, a push-pull fluorescent dye for lipid droplet imaging
citation_author	Wee, Wen Ann
	Andini, Tatum Melati
	Kumagai, Tomotaka
	Chinnathambi, Shanmugavel
	Pandian, Ganesh N.
	Millius, Arthur
	Sugiyama, Hiroshi
	Standley, Daron M.
	Park, Soyoung
citation_publisher	Elsevier B.V.
citation_language	英語
citation_date	2025-05-03
citation_journal_title	Analytica Chimica Acta
citation_volume	1359
citation_firstpage	344130
citation_doi	https://doi.org/10.1016/j.aca.2025.344130
citation_public_url	https://hdl.handle.net/11094/102199

Tiny but mighty! N,N-dimethyl-4-(5-nitrothiophen-2-yl)aniline, a push-pull fluorescent dye for lipid droplet imaging

閲覧可能ファイル

論文情報

論文詳細を表示