Id2 transcriptional repressor promotes retinal Müller glial cell fate choice by increasing Notch activity

Fujieda, Kazuki; Sumihiro, Hitoshi; Saito, Rika; Kobayashi, Koki; Miyanohara, Yuki; Abe, Shinya; Tsujii, Toshinori; Tu, Hung Ya; Furukawa, Takahisa

doi:https://doi.org/10.1016/j.bbrc.2025.151966

Id2 transcriptional repressor promotes retinal Müller glial cell fate choice by increasing Notch activity

Fujieda, Kazuki; Sumihiro, Hitoshi; Saito, Rika et al.

Biochemical and Biophysical Research Communications, 2025, 771, 151966

アクセス数:107件（2025-12-04 19:40 集計）

固定URL: https://hdl.handle.net/11094/102201

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タイトル	Id2 transcriptional repressor promotes retinal Müller glial cell fate choice by increasing Notch activity
著者	著者名 Fujieda, Kazuki
	著者名 Sumihiro, Hitoshi
	著者名 Saito, Rika
	著者名 Kobayashi, Koki
	著者名 Miyanohara, Yuki
	著者名 Abe, Shinya
	著者名 Tsujii, Toshinori
	NRID 1000010780835 1000010780835 ORCID 0000-0002-4868-6262 0000-0002-4868-6262 researchmap permalink hungyatu hungyatu 著者名 Tu, Hung Ya
	NRID 1000050260609 1000050260609 ORCID 0000-0003-4171-220X 0000-0003-4171-220X researchmap permalink read0083689 read0083689 Scopus Author ID 7403160171 7403160171 著者名 Furukawa, Takahisa
内容	Fujieda K., Sumihiro H., Saito R., et al. Id2 transcriptional repressor promotes retinal Müller glial cell fate choice by increasing Notch activity. Biochemical and Biophysical Research Communications 771, 151966 (2025); https://doi.org/10.1016/j.bbrc.2025.151966.
抄録	In the vertebrate retina, Müller glial cells and neurons originate from common progenitor cells. To identify the genes that regulate the cell fate choice of Müller glial cells from retinal progenitor cells, we first generated Rlbp1-EGFP transgenic mice in which differentiating Müller glia were specifically labeled with EGFP. By performing fluorescence-activated cell sorting and RNA sequencing analysis, we found that Id1, Id2, and Id3, were highly enriched in developing and differentiated Müller glial cells. While Id1/2/3 are inhibitory helix-loop-helix (HLH) transcription factors, the functional role and underlying mechanism of each Id protein in Müller glial cell development are not fully understood. In situ hybridization revealed Id1/2/3 expression in the layer where retinal progenitor cells and Müller glial cells reside in developing and adult retinas, respectively. Cell lineage analysis using retroviruses expressing Id1, Id2, or Id3 showed that Id2 possesses a stronger inducing activity in Müller glial cell fate choice than Id1 and Id3. Co-immunoprecipitation and luciferase reporter analyses suggested that Id2 interacts with the Notch intracellular region and promotes Notch activity. Taken together, these results suggest that Id2 promotes Müller glial cell fate choice by increasing Notch activity in the developing mouse retina.
出版者	Elsevier B.V.
収録物名	Biochemical and Biophysical Research Communications
巻(号)	771
ページ	151966
刊行年月	2025-05-20
言語	英語
ハンドルURL	https://hdl.handle.net/11094/102201
PISSN	0006291X
eISSN	10902104
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1016/j.bbrc.2025.151966
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Biochemical and Biophysical Research Communications. 2025, 771, p. 151966
DC.title	Id2 transcriptional repressor promotes retinal Müller glial cell fate choice by increasing Notch activity
DC.creator	Fujieda, Kazuki
	Sumihiro, Hitoshi
	Saito, Rika
	Kobayashi, Koki
	Miyanohara, Yuki
	Abe, Shinya
	Tsujii, Toshinori
	Tu, Hung Ya
	Furukawa, Takahisa
DC.publisher	Elsevier B.V.
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2025-05-20
DC.identifier	https://doi.org/10.1016/j.bbrc.2025.151966
DC.description	Fujieda K., Sumihiro H., Saito R., et al. Id2 transcriptional repressor promotes retinal Müller glial cell fate choice by increasing Notch activity. Biochemical and Biophysical Research Communications 771, 151966 (2025); https://doi.org/10.1016/j.bbrc.2025.151966.
DCTERMS.abstract	In the vertebrate retina, Müller glial cells and neurons originate from common progenitor cells. To identify the genes that regulate the cell fate choice of Müller glial cells from retinal progenitor cells, we first generated Rlbp1-EGFP transgenic mice in which differentiating Müller glia were specifically labeled with EGFP. By performing fluorescence-activated cell sorting and RNA sequencing analysis, we found that Id1, Id2, and Id3, were highly enriched in developing and differentiated Müller glial cells. While Id1/2/3 are inhibitory helix-loop-helix (HLH) transcription factors, the functional role and underlying mechanism of each Id protein in Müller glial cell development are not fully understood. In situ hybridization revealed Id1/2/3 expression in the layer where retinal progenitor cells and Müller glial cells reside in developing and adult retinas, respectively. Cell lineage analysis using retroviruses expressing Id1, Id2, or Id3 showed that Id2 possesses a stronger inducing activity in Müller glial cell fate choice than Id1 and Id3. Co-immunoprecipitation and luciferase reporter analyses suggested that Id2 interacts with the Notch intracellular region and promotes Notch activity. Taken together, these results suggest that Id2 promotes Müller glial cell fate choice by increasing Notch activity in the developing mouse retina.
DC.rights	This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
DC.format	102201
citation_title	Id2 transcriptional repressor promotes retinal Müller glial cell fate choice by increasing Notch activity
citation_author	Fujieda, Kazuki
	Sumihiro, Hitoshi
	Saito, Rika
	Kobayashi, Koki
	Miyanohara, Yuki
	Abe, Shinya
	Tsujii, Toshinori
	Tu, Hung Ya
	Furukawa, Takahisa
citation_publisher	Elsevier B.V.
citation_language	英語
citation_date	2025-05-20
citation_journal_title	Biochemical and Biophysical Research Communications
citation_volume	771
citation_firstpage	151966
citation_doi	https://doi.org/10.1016/j.bbrc.2025.151966
citation_public_url	https://hdl.handle.net/11094/102201

Id2 transcriptional repressor promotes retinal Müller glial cell fate choice by increasing Notch activity

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