Development and characterization of MRC-5 and Vero cell-adapted enterovirus D68 strains for vaccine production

Senpuku, Kota; Kunishima, Yuta; Taniguchi, Kotaro; Ito, Taiki; Hirai, Toshiro; Nakamura, Teruya; Kataoka-Nakamura, Chikako; Yoshioka, Yasuo

doi:https://doi.org/10.1016/j.vaccine.2025.127314

Development and characterization of MRC-5 and Vero cell-adapted enterovirus D68 strains for vaccine production

Senpuku, Kota; Kunishima, Yuta; Taniguchi, Kotaro et al.

Vaccine, 2025, 60, 127314

アクセス数:14件（2025-09-29 14:23 集計）

固定URL: https://hdl.handle.net/11094/102604

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タイトル	Development and characterization of MRC-5 and Vero cell-adapted enterovirus D68 strains for vaccine production
著者	著者名 Senpuku, Kota
	著者名 Kunishima, Yuta
	著者名 Taniguchi, Kotaro
	NRID 1000081019818 1000081019818 ORCID 0000-0002-1408-7288 0000-0002-1408-7288 researchmap permalink ito_taiki ito_taiki 著者名 Ito, Taiki
	NRID 1000000895815 1000000895815 ORCID 0000-0002-9212-1664 0000-0002-9212-1664 researchmap permalink toshiro.hirai toshiro.hirai Scopus Author ID 55628552543 55628552543 著者名 Hirai, Toshiro
	NRID 1000040433015 1000040433015 著者名 Nakamura, Teruya
	NRID 1000010646623 1000010646623 著者名 Kataoka-Nakamura, Chikako
	著者名 Yoshioka, Yasuo
キーワード等	Enterovirus D68
	MRC-5 cells
	Vaccine
	Vaccine manufacturing
	Vero cells
内容	Senpuku K., Kunishima Y., Taniguchi K., et al. Development and characterization of MRC-5 and Vero cell-adapted enterovirus D68 strains for vaccine production. Vaccine 60, 127314 (2025); https://doi.org/10.1016/j.vaccine.2025.127314.
抄録	Enterovirus D68 (EV-D68) is a pathogen that causes respiratory and neurological diseases. Currently, there are no licensed vaccines for EV-D68. Here, we adapted EV-D68 to MRC-5 and Vero cells, which are widely used in vaccine manufacturing, to develop EV-D68 strains applicable for vaccine production. We successfully isolated MRC-5 cell-adapted strains by serial passaging in MRC-5 cells. Although efforts to isolate Vero cell-adapted strains through serial passaging of EV-D68 in Vero cells were unsuccessful, we isolated Vero cell-adapted strains by serial passaging of MRC-5 cell-adapted strains in Vero cells. Inactivated whole-virion vaccines were prepared from vaccine-manufacturing cell-adapted strains and mice were immunized with these vaccines. We found that in some cases, the parental and cell-adapted strains induced similar levels of protective immunity against EV-D68, whereas in other cases, the cell-adapted strains were significantly less effective than the parental strains. These data provide valuable information for EV-D68 vaccine production.
出版者	Elsevier Ltd
収録物名	Vaccine
巻(号)	60
ページ	127314
刊行年月	2025-05-26
言語	英語
ハンドルURL	https://hdl.handle.net/11094/102604
PISSN	0264410X
eISSN	18732518
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1016/j.vaccine.2025.127314
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Vaccine. 2025, 60, p. 127314
DC.title	Development and characterization of MRC-5 and Vero cell-adapted enterovirus D68 strains for vaccine production
DC.creator	Senpuku, Kota
	Kunishima, Yuta
	Taniguchi, Kotaro
	Ito, Taiki
	Hirai, Toshiro
	Nakamura, Teruya
	Kataoka-Nakamura, Chikako
	Yoshioka, Yasuo
DC.publisher	Elsevier Ltd
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2025-05-26
DC.identifier	https://doi.org/10.1016/j.vaccine.2025.127314
DC.subject	Enterovirus D68
	MRC-5 cells
	Vaccine
	Vaccine manufacturing
	Vero cells
DC.description	Senpuku K., Kunishima Y., Taniguchi K., et al. Development and characterization of MRC-5 and Vero cell-adapted enterovirus D68 strains for vaccine production. Vaccine 60, 127314 (2025); https://doi.org/10.1016/j.vaccine.2025.127314.
DCTERMS.abstract	Enterovirus D68 (EV-D68) is a pathogen that causes respiratory and neurological diseases. Currently, there are no licensed vaccines for EV-D68. Here, we adapted EV-D68 to MRC-5 and Vero cells, which are widely used in vaccine manufacturing, to develop EV-D68 strains applicable for vaccine production. We successfully isolated MRC-5 cell-adapted strains by serial passaging in MRC-5 cells. Although efforts to isolate Vero cell-adapted strains through serial passaging of EV-D68 in Vero cells were unsuccessful, we isolated Vero cell-adapted strains by serial passaging of MRC-5 cell-adapted strains in Vero cells. Inactivated whole-virion vaccines were prepared from vaccine-manufacturing cell-adapted strains and mice were immunized with these vaccines. We found that in some cases, the parental and cell-adapted strains induced similar levels of protective immunity against EV-D68, whereas in other cases, the cell-adapted strains were significantly less effective than the parental strains. These data provide valuable information for EV-D68 vaccine production.
DC.rights	This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
DC.format	102604
citation_title	Development and characterization of MRC-5 and Vero cell-adapted enterovirus D68 strains for vaccine production
citation_author	Senpuku, Kota
	Kunishima, Yuta
	Taniguchi, Kotaro
	Ito, Taiki
	Hirai, Toshiro
	Nakamura, Teruya
	Kataoka-Nakamura, Chikako
	Yoshioka, Yasuo
citation_publisher	Elsevier Ltd
citation_language	英語
citation_date	2025-05-26
citation_journal_title	Vaccine
citation_volume	60
citation_firstpage	127314
citation_doi	https://doi.org/10.1016/j.vaccine.2025.127314
citation_public_url	https://hdl.handle.net/11094/102604

Development and characterization of MRC-5 and Vero cell-adapted enterovirus D68 strains for vaccine production

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