Chemical Synthesis of NICOL, A Coligand Secreted Protein Mediating Mammalian Male Reproductive Tract Trans-luminal Signaling

Nagahama, Kenta; Takei, Toshiki; Ito, Shun; Takao, Toshifumi; Hojo, Hironobu; Kiyozumi, Daiji

doi:https://doi.org/10.1002/chem.202501588

Chemical Synthesis of NICOL, A Coligand Secreted Protein Mediating Mammalian Male Reproductive Tract Trans-luminal Signaling

Nagahama, Kenta; Takei, Toshiki; Ito, Shun et al.

Chemistry - A European Journal, 2025, 31(47), e01588

アクセス数:219件（2026-02-15 21:48 集計）

固定URL: https://hdl.handle.net/11094/102754

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タイトル	Chemical Synthesis of NICOL, A Coligand Secreted Protein Mediating Mammalian Male Reproductive Tract Trans-luminal Signaling
著者	著者名 Nagahama, Kenta
	NRID 1000000844771 1000000844771 researchmap permalink toshiki.takei toshiki.takei Scopus Author ID 56010416500 56010416500 著者名 Takei, Toshiki
	著者名 Ito, Shun
	NRID 1000010197048 1000010197048 ORCID 0000-0002-4941-922X 0000-0002-4941-922X researchmap permalink Toshifumi_Takao Toshifumi_Takao Scopus Author ID 7102574661 7102574661 著者名 Takao, Toshifumi
	NRID 1000000209214 1000000209214 researchmap permalink read0014254 read0014254 Scopus Author ID 7102337557 7102337557 ORCID 0000-0003-0916-1233 0000-0003-0916-1233 著者名 Hojo, Hironobu
	NRID 1000070452430 1000070452430 著者名 Kiyozumi, Daiji
キーワード等	chemical synthesis
	lumicrine
	NELL2
	NICOL
内容	Nagahama K., Takei T., Ito S., et al. Chemical Synthesis of NICOL, A Coligand Secreted Protein Mediating Mammalian Male Reproductive Tract Trans-luminal Signaling. Chemistry - A European Journal 31, e01588 (2025); https://doi.org/10.1002/chem.202501588.
抄録	The NELL2-interacting cofactor for lumicrine signaling (NICOL) is a small protein which forms a complex with the neural epidermal growth factor–like like 2 (NELL2) and engaged in sperm maturation in the epididymis. At present, the recombinant expression of NICOL is not efficient, which limits the precise understanding of the structure and function of NICOL. In this study, we first succeeded in the chemical synthesis of NICOL with NELL2 binding affinity. The synthesized NICOL possesses correct SS bond pairing, and its secondary structure is indistinguishable from that of the recombinant protein, indicating that the synthetic NICOL will be useful for structural and functional analyses of lumicrine signaling.
出版者	John Wiley and Sons Inc
収録物名	Chemistry - A European Journal
巻(号)	31(47)
ページ	e01588
刊行年月	2025-07-26
言語	英語
ハンドルURL	https://hdl.handle.net/11094/102754
PISSN	09476539
eISSN	15213765
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1002/chem.202501588
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Chemistry - A European Journal. 2025, 31(47), p. e01588
DC.title	Chemical Synthesis of NICOL, A Coligand Secreted Protein Mediating Mammalian Male Reproductive Tract Trans-luminal Signaling
DC.creator	Nagahama, Kenta
	Takei, Toshiki
	Ito, Shun
	Takao, Toshifumi
	Hojo, Hironobu
	Kiyozumi, Daiji
DC.publisher	John Wiley and Sons Inc
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2025-07-26
DC.identifier	https://doi.org/10.1002/chem.202501588
DC.subject	chemical synthesis
	lumicrine
	NELL2
	NICOL
DC.description	Nagahama K., Takei T., Ito S., et al. Chemical Synthesis of NICOL, A Coligand Secreted Protein Mediating Mammalian Male Reproductive Tract Trans-luminal Signaling. Chemistry - A European Journal 31, e01588 (2025); https://doi.org/10.1002/chem.202501588.
DCTERMS.abstract	The NELL2-interacting cofactor for lumicrine signaling (NICOL) is a small protein which forms a complex with the neural epidermal growth factor–like like 2 (NELL2) and engaged in sperm maturation in the epididymis. At present, the recombinant expression of NICOL is not efficient, which limits the precise understanding of the structure and function of NICOL. In this study, we first succeeded in the chemical synthesis of NICOL with NELL2 binding affinity. The synthesized NICOL possesses correct SS bond pairing, and its secondary structure is indistinguishable from that of the recombinant protein, indicating that the synthetic NICOL will be useful for structural and functional analyses of lumicrine signaling.
DC.rights	This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
DC.format	102754
citation_title	Chemical Synthesis of NICOL, A Coligand Secreted Protein Mediating Mammalian Male Reproductive Tract Trans-luminal Signaling
citation_author	Nagahama, Kenta
	Takei, Toshiki
	Ito, Shun
	Takao, Toshifumi
	Hojo, Hironobu
	Kiyozumi, Daiji
citation_publisher	John Wiley and Sons Inc
citation_language	英語
citation_date	2025-07-26
citation_journal_title	Chemistry - A European Journal
citation_volume	31
citation_issue	47
citation_firstpage	e01588
citation_doi	https://doi.org/10.1002/chem.202501588
citation_public_url	https://hdl.handle.net/11094/102754

Chemical Synthesis of NICOL, A Coligand Secreted Protein Mediating Mammalian Male Reproductive Tract Trans-luminal Signaling

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