Metabolic Engineering of the 5-Aminolevulinate Biosynthetic Pathway in E. coli Improves Efficiency of Hemoprotein-Based Biocatalysis

Kato, Shunsuke; Abe, Miteki; Okahashi, Nobuyuki et al.

Angewandte Chemie - International Edition, 2025, e202512156

アクセス数:28件（2025-10-18 01:18 集計）

固定URL: https://hdl.handle.net/11094/102758

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タイトル
Metabolic Engineering of the 5-Aminolevulinate Biosynthetic Pathway in E. coli Improves Efficiency of Hemoprotein-Based Biocatalysis
著者
NRID
1000060909125 1000060909125
researchmap permalink
s_kato_chem s_kato_chem
Scopus Author ID
57195564115 57195564115
ORCID
0009-0003-6824-612X 0009-0003-6824-612X
著者名
Kato, Shunsuke
著者名
Abe, Miteki
NRID
1000030802748 1000030802748
ORCID
0000-0001-9582-355X 0000-0001-9582-355X
researchmap permalink
okahashinobuyuki okahashinobuyuki
Scopus Author ID
56549714700 56549714700
著者名
Okahashi, Nobuyuki
NRID
1000050586998 1000050586998
著者名
Ariyasu, Shinya
NRID
1000050462734 1000050462734
ORCID
0000-0003-1091-778X 0000-0003-1091-778X
researchmap permalink
fumio_matsuda fumio_matsuda
Scopus Author ID
7202165617 7202165617
著者名
Matsuda, Fumio
NRID
1000090379587 1000090379587
著者名
Shoji, Osami
NRID
1000020222226 1000020222226
ORCID
0000-0002-2215-935X 0000-0002-2215-935X
researchmap permalink
20190602_Sun 20190602_Sun
Scopus Author ID
55735983400 55735983400
著者名
Hayashi, Takashi
キーワード等
5-Aminolevulinic acid
Biocatalysis
Heme
Hemoprotein
Metabolic engineering
内容
Kato S., Abe M., Okahashi N., et al. Metabolic Engineering of the 5-Aminolevulinate Biosynthetic Pathway in E. coli Improves Efficiency of Hemoprotein-Based Biocatalysis. Angewandte Chemie - International Edition (2025); https://doi.org/10.1002/anie.202512156.
抄録
Biocatalysis using heme-dependent enzymes provides a powerful synthetic platform to facilitate a variety of chemical transformations required for organic synthesis. Despite recent advances in biocatalysis, recombinant expression systems for hemoproteins leave much room for improvement due to the strict regulation of heme biosynthesis in the host organism. To develop an efficient cofactor supplementation system for the expression of active holohemoproteins, we describe metabolic engineering of the heme biosynthetic pathway in E. coli. Through incorporation of a heterogeneous C4 pathway involving 5-aminolevulinic acid synthase of Paracoccus denitrificans, it was found that the concentrations of 5-aminolevulinic acid and heme in the engineered cells are increased during cultivation, and the expression level of the holohemoproteins is significantly improved. Notably, the heme content in the engineered cells is even higher than that produced by conventional cultivation methods, which add 5-aminolevulinic acid into the culture medium. Furthermore, we also demonstrate the application of this engineered E. coli cells in whole-cell and lysate-based biocatalysis using various types of heme-dependent enzymes. Considering the recent demand for biocatalysis, the system developed in this study will serve as a new practical and versatile platform for hemoprotein-based biocatalysis.
出版者
John Wiley and Sons Inc
収録物名
Angewandte Chemie - International Edition
ページ
e202512156
刊行年月
2025-08-05
言語
英語
ハンドルURL
https://hdl.handle.net/11094/102758
PISSN
14337851
eISSN
15213773
関連情報 (isIdenticalTo)
DOI (出版社版)
https://doi.org/10.1002/anie.202512156
アクセス権
open access
権利情報
This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
出版タイプ
Version of Record
カテゴリ
学術雑誌論文 Journal Article