Mapping of projection of sprouting neuron in neonate and adult mice after pyramidotomy

Tsujioka, Hiroshi; Yamashita, Toshihide

doi:https://doi.org/10.1016/j.expneurol.2025.115386

Mapping of projection of sprouting neuron in neonate and adult mice after pyramidotomy

Tsujioka, Hiroshi; Yamashita, Toshihide

Experimental Neurology, 2025, 393, 115386

アクセス数:8件（2025-10-24 17:51 集計）

固定URL: https://hdl.handle.net/11094/102776

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タイトル	Mapping of projection of sprouting neuron in neonate and adult mice after pyramidotomy
著者	NRID 1000020803505 1000020803505 ORCID 0000-0001-7484-4191 0000-0001-7484-4191 researchmap permalink tsujioka tsujioka Scopus Author ID 57188553938 57188553938 著者名 Tsujioka, Hiroshi
著者	NRID 1000010301269 1000010301269 ORCID 0000-0003-4559-7018 0000-0003-4559-7018 researchmap permalink ToshihideYamashita ToshihideYamashita Scopus Author ID 35479670200 35479670200 著者名 Yamashita, Toshihide
キーワード等	Corticospinal tract
	MAPseq
	Neonate
	Pyramidotomy
	Sprouting
内容	Tsujioka H., Yamashita T.. Mapping of projection of sprouting neuron in neonate and adult mice after pyramidotomy. Experimental Neurology 393, 115386 (2025); https://doi.org/10.1016/j.expneurol.2025.115386.
抄録	Although severed axons of the corticospinal tract rarely regenerate, neural function is partially recovered by axonal sprouting from intact neurons. The sprouting capability is higher in neonates than in adults, making neonates an attractive model for finding novel therapeutic targets for central nervous system injury. The axonal projection pattern of the motor cortex neurons is highly heterogeneous, and high-throughput analysis at the single-cell level is required to correctly understand the projection pattern of sprouting neurons. The recently developed multiplexed analysis of projections by sequencing (MAPseq) has revealed axonal projection complexity in many brain areas; however, axonal sprouting of the corticospinal tract in neonates and adults has not yet been analyzed. Here, we evaluated the sprouting pattern of individual neurons using MAPseq in neonate and adult mice after pyramidotomy, using the intact and denervated (sprouting) sides of the cervical cord. The ratio of neurons projecting solely to the intact or denervated side or projecting to both sides was not significantly different. The cumulative projection strength on the denervated side was significantly higher in the neonatal pyramidotomy group than in the other groups. The ratio of the projection strength on the denervated side to the total projection strength (sprouting index) correlated with the projection strength on the denervated side. The ratio of the neuron with a sprouting index of 0.6–0.7 was significantly higher in the neonatal pyramidotomy group. These results show the usefulness of MAPseq in sprouting studies and provide important information regarding the sprouting patterns of individual neurons in neonates.
出版者	Elsevier Inc.
収録物名	Experimental Neurology
巻(号)	393
ページ	115386
刊行年月	2025-11-01
言語	英語
ハンドルURL	https://hdl.handle.net/11094/102776
PISSN	00144886
eISSN	10902430
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1016/j.expneurol.2025.115386
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution 4.0 International License.
権利情報	© 2025 The Authors
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Experimental Neurology. 2025, 393, p. 115386
DC.title	Mapping of projection of sprouting neuron in neonate and adult mice after pyramidotomy
DC.creator	Tsujioka, Hiroshi
DC.creator	Yamashita, Toshihide
DC.publisher	Elsevier Inc.
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2025-11-01
DC.identifier	https://doi.org/10.1016/j.expneurol.2025.115386
DC.subject	Corticospinal tract
	MAPseq
	Neonate
	Pyramidotomy
	Sprouting
DC.description	Tsujioka H., Yamashita T.. Mapping of projection of sprouting neuron in neonate and adult mice after pyramidotomy. Experimental Neurology 393, 115386 (2025); https://doi.org/10.1016/j.expneurol.2025.115386.
DCTERMS.abstract	Although severed axons of the corticospinal tract rarely regenerate, neural function is partially recovered by axonal sprouting from intact neurons. The sprouting capability is higher in neonates than in adults, making neonates an attractive model for finding novel therapeutic targets for central nervous system injury. The axonal projection pattern of the motor cortex neurons is highly heterogeneous, and high-throughput analysis at the single-cell level is required to correctly understand the projection pattern of sprouting neurons. The recently developed multiplexed analysis of projections by sequencing (MAPseq) has revealed axonal projection complexity in many brain areas; however, axonal sprouting of the corticospinal tract in neonates and adults has not yet been analyzed. Here, we evaluated the sprouting pattern of individual neurons using MAPseq in neonate and adult mice after pyramidotomy, using the intact and denervated (sprouting) sides of the cervical cord. The ratio of neurons projecting solely to the intact or denervated side or projecting to both sides was not significantly different. The cumulative projection strength on the denervated side was significantly higher in the neonatal pyramidotomy group than in the other groups. The ratio of the projection strength on the denervated side to the total projection strength (sprouting index) correlated with the projection strength on the denervated side. The ratio of the neuron with a sprouting index of 0.6–0.7 was significantly higher in the neonatal pyramidotomy group. These results show the usefulness of MAPseq in sprouting studies and provide important information regarding the sprouting patterns of individual neurons in neonates.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.rights	© 2025 The Authors
DC.format	102776
citation_title	Mapping of projection of sprouting neuron in neonate and adult mice after pyramidotomy
citation_author	Tsujioka, Hiroshi
citation_author	Yamashita, Toshihide
citation_publisher	Elsevier Inc.
citation_language	英語
citation_date	2025-11-01
citation_journal_title	Experimental Neurology
citation_volume	393
citation_firstpage	115386
citation_doi	https://doi.org/10.1016/j.expneurol.2025.115386
citation_public_url	https://hdl.handle.net/11094/102776

Mapping of projection of sprouting neuron in neonate and adult mice after pyramidotomy

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