Freeze-dryable inks combining chitosan nanofibers and hyaluronic acid for extrusion bioprinting

Hirami, Ryo; Sakai, Shinji

International Journal of Biological Macromolecules, 2025, 322, 146679

アクセス数:20件（2025-12-07 18:17 集計）

固定URL: https://hdl.handle.net/11094/102786

閲覧可能ファイル 

ファイル フォーマット 利用条件 サイズ 閲覧回数 利用開始日 説明 information
IntJBiolMacromol_322 pdf なし 6.52 MB 28 2025.09.26  
Creative Commons : 表示

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

タイトル
Freeze-dryable inks combining chitosan nanofibers and hyaluronic acid for extrusion bioprinting
著者
著者名
Hirami, Ryo
NRID
1000020359938 1000020359938
ORCID
0000-0002-1041-4798 0000-0002-1041-4798
researchmap permalink
sakaishinji sakaishinji
Scopus Author ID
35450082900 35450082900
著者名
Sakai, Shinji
キーワード等
Chitosan nanofibers
Freeze-dryable ink
Three-dimensional bioprinting
内容
Hirami R., Sakai S.. Freeze-dryable inks combining chitosan nanofibers and hyaluronic acid for extrusion bioprinting. International Journal of Biological Macromolecules 322, 146679 (2025); https://doi.org/10.1016/j.ijbiomac.2025.146679.
抄録
Freeze-dryable inks offer significant advantages for bioprinting, such as long-term biochemical and biophysical stability with minimal risks of hydrolysis, denaturation, and microbial contamination. In this study, we developed inks that can be stored in a ready-to-use, freeze-dried form by incorporating chitosan nanofibers (ChitoNFs), sodium hyaluronic acid (HA-Na), and phenolated HA (HA-Ph). HA-Na and HA-Ph effectively inhibited ChitoNF aggregation during drying. Upon rehydration, the developed inks exhibited shear-thinning behavior and underwent horseradish peroxidase-catalyzed gelation. The optimization of the ChitoNF, HA-Na, and HA-Ph concentrations enabled precise control of the viscosity, gelation time, and hydrogel stiffness after rehydration. Using 0.8 w/v% HA-Na, 0.2 w/v% HA-Ph, and 1.5 w/v% ChitoNF, we achieved high-fidelity three-dimensional printing of constructs. Furthermore, the incorporation of 0.5 w/v% phenolated gelatin supported the encapsulation of 10 T1/2 fibroblasts, resulting in more than 90 % viability and a 3.1-fold increase in mitochondrial activity over 7 d. Our findings revealed that pre-mixed, freeze-dryable ChitoNF–HA-Na/HA-Ph bioinks offer a practical and innovative solution for bioprinting. By ensuring long-term storability, quantitative retention of print fidelity and cytocompatibility after rehydration, and simplified logistics for clinical translation, they address unmet needs in current bioink technologies.
出版者
Elsevier B.V.
収録物名
International Journal of Biological Macromolecules
巻(号)
322
ページ
146679
刊行年月
2025-09-01
言語
英語
ハンドルURL
https://hdl.handle.net/11094/102786
PISSN
01418130
eISSN
18790003
関連情報 (isIdenticalTo)
DOI (出版社版)
https://doi.org/10.1016/j.ijbiomac.2025.146679
アクセス権
open access
権利情報
This article is licensed under a Creative Commons Attribution 4.0 International License.
© 2025 The Authors
出版タイプ
Version of Record
カテゴリ
学術雑誌論文 Journal Article