Adaptor protein complex 1 facilitates ciliary localization of serotonin receptor type 6

Qin, Yuanyuan; Miyoshi, Ko; Yinsheng, Zhuoma; Fujiwara, Yuuki; Yoshimura, Takeshi; Katayama, Taiichi

doi:https://doi.org/10.1016/j.cellsig.2025.112008

Adaptor protein complex 1 facilitates ciliary localization of serotonin receptor type 6

Qin, Yuanyuan; Miyoshi, Ko; Yinsheng, Zhuoma et al.

Cellular Signalling, 2025, 135, 112008

アクセス数:17件（2025-12-03 16:54 集計）

固定URL: https://hdl.handle.net/11094/102856

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タイトル	Adaptor protein complex 1 facilitates ciliary localization of serotonin receptor type 6
著者	著者名 Qin, Yuanyuan
	NRID 1000090362996 1000090362996 researchmap permalink ko_miyoshi ko_miyoshi Scopus Author ID 7202855230 7202855230 著者名 Miyoshi, Ko
	著者名 Yinsheng, Zhuoma
	NRID 1000090794925 1000090794925 ORCID 0000-0003-1032-7755 0000-0003-1032-7755 researchmap permalink yuuki-fujiwara yuuki-fujiwara Scopus Author ID 57202568656 57202568656 著者名 Fujiwara, Yuuki
	NRID 1000060402567 1000060402567 researchmap permalink takeshiyoshimura takeshiyoshimura Scopus Author ID 57080284500 57080284500 著者名 Yoshimura, Takeshi
	NRID 1000080333459 1000080333459 ORCID 0000-0001-8137-0481 0000-0001-8137-0481 researchmap permalink read0207195 read0207195 Scopus Author ID 7401478639 7401478639 著者名 Katayama, Taiichi
キーワード等	AP-1
	G protein-coupled receptors
	Primary cilia
	Serotonin receptor type 6
	γ1-Adaptin
内容	Qin Y., Miyoshi K., Yinsheng Z., et al. Adaptor protein complex 1 facilitates ciliary localization of serotonin receptor type 6. Cellular Signalling 135, 112008 (2025); https://doi.org/10.1016/j.cellsig.2025.112008.
抄録	The primary cilium, an immotile protrusion of vertebrate cells, detects chemical and mechanical stimuli in the extracellular milieu and transduces them into the cell body, thereby contributing to cellular development and homeostasis. In the mammalian brain, serotonin receptor type 6 (Htr6) and other specific G protein-coupled receptors (GPCRs) localize preferentially to primary cilia and function in ciliary chemical detection; however, the molecular mechanism by which a subset of GPCRs is transported to primary cilia has not been fully elucidated. In the present study, we demonstrate that a region in the fourth intracellular domain of Htr6 (Htr6 i4) is sufficient for ciliary localization. In yeast, the interaction of this region with the C-terminal region of γ1-Adaptin, a subunit of adaptor protein complex 1 (AP-1), was identified. The interaction between Htr6 and γ1-Adaptin was confirmed by immunoprecipitation analysis using HEK293T cells. The preference for ciliary localization of Htr6 and Htr6 i4 was significantly decreased by ablation of γ1-Adaptin in hTERT RPE-1 cells, which was rescued by exogenous expression of γ1-Adaptin. Furthermore, Htr6 and Htr6 i4 showed reduced localization to primary cilia in γ1-Adaptin-depleted cultured hippocampal neurons compared with control neurons. These results indicate that the ciliary localization of Htr6 is facilitated by AP-1-mediated membrane trafficking.
出版者	Elsevier Inc.
収録物名	Cellular Signalling
巻(号)	135
ページ	112008
刊行年月	2025-11-01
言語	英語
ハンドルURL	https://hdl.handle.net/11094/102856
PISSN	08986568
eISSN	18733913
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1016/j.cellsig.2025.112008
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Cellular Signalling. 2025, 135, p. 112008
DC.title	Adaptor protein complex 1 facilitates ciliary localization of serotonin receptor type 6
DC.creator	Qin, Yuanyuan
	Miyoshi, Ko
	Yinsheng, Zhuoma
	Fujiwara, Yuuki
	Yoshimura, Takeshi
	Katayama, Taiichi
DC.publisher	Elsevier Inc.
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2025-11-01
DC.identifier	https://doi.org/10.1016/j.cellsig.2025.112008
DC.subject	AP-1
	G protein-coupled receptors
	Primary cilia
	Serotonin receptor type 6
	γ1-Adaptin
DC.description	Qin Y., Miyoshi K., Yinsheng Z., et al. Adaptor protein complex 1 facilitates ciliary localization of serotonin receptor type 6. Cellular Signalling 135, 112008 (2025); https://doi.org/10.1016/j.cellsig.2025.112008.
DCTERMS.abstract	The primary cilium, an immotile protrusion of vertebrate cells, detects chemical and mechanical stimuli in the extracellular milieu and transduces them into the cell body, thereby contributing to cellular development and homeostasis. In the mammalian brain, serotonin receptor type 6 (Htr6) and other specific G protein-coupled receptors (GPCRs) localize preferentially to primary cilia and function in ciliary chemical detection; however, the molecular mechanism by which a subset of GPCRs is transported to primary cilia has not been fully elucidated. In the present study, we demonstrate that a region in the fourth intracellular domain of Htr6 (Htr6 i4) is sufficient for ciliary localization. In yeast, the interaction of this region with the C-terminal region of γ1-Adaptin, a subunit of adaptor protein complex 1 (AP-1), was identified. The interaction between Htr6 and γ1-Adaptin was confirmed by immunoprecipitation analysis using HEK293T cells. The preference for ciliary localization of Htr6 and Htr6 i4 was significantly decreased by ablation of γ1-Adaptin in hTERT RPE-1 cells, which was rescued by exogenous expression of γ1-Adaptin. Furthermore, Htr6 and Htr6 i4 showed reduced localization to primary cilia in γ1-Adaptin-depleted cultured hippocampal neurons compared with control neurons. These results indicate that the ciliary localization of Htr6 is facilitated by AP-1-mediated membrane trafficking.
DC.rights	This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
DC.format	102856
citation_title	Adaptor protein complex 1 facilitates ciliary localization of serotonin receptor type 6
citation_author	Qin, Yuanyuan
	Miyoshi, Ko
	Yinsheng, Zhuoma
	Fujiwara, Yuuki
	Yoshimura, Takeshi
	Katayama, Taiichi
citation_publisher	Elsevier Inc.
citation_language	英語
citation_date	2025-11-01
citation_journal_title	Cellular Signalling
citation_volume	135
citation_firstpage	112008
citation_doi	https://doi.org/10.1016/j.cellsig.2025.112008
citation_public_url	https://hdl.handle.net/11094/102856

Adaptor protein complex 1 facilitates ciliary localization of serotonin receptor type 6

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