Hypertonic intranasal vaccines gain nasal epithelia access to exert strong immunogenicity

Hashimoto, Soichiro; Hirai, Toshiro; Ueda, Koki; Kakihara, Mako; Tokunoh, Nagisa; Ono, Chikako; Matsuura, Yoshiharu; Takayama, Kazuo; Yoshioka, Yasuo

doi:https://doi.org/10.1016/j.mucimm.2025.03.006

Hypertonic intranasal vaccines gain nasal epithelia access to exert strong immunogenicity

Hashimoto, Soichiro; Hirai, Toshiro; Ueda, Koki et al.

Mucosal Immunology, 2025, 18(4), 793-809

アクセス数:21件（2025-12-03 09:15 集計）

固定URL: https://hdl.handle.net/11094/102928

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タイトル	Hypertonic intranasal vaccines gain nasal epithelia access to exert strong immunogenicity
著者	著者名 Hashimoto, Soichiro
	NRID 1000000895815 1000000895815 ORCID 0000-0002-9212-1664 0000-0002-9212-1664 researchmap permalink toshiro.hirai toshiro.hirai Scopus Author ID 55628552543 55628552543 著者名 Hirai, Toshiro
	著者名 Ueda, Koki
	著者名 Kakihara, Mako
	著者名 Tokunoh, Nagisa
	NRID 1000030626437 1000030626437 researchmap permalink ChikakoOno ChikakoOno Scopus Author ID 54990934700 54990934700 著者名 Ono, Chikako
	NRID 1000050157252 1000050157252 researchmap permalink matsuura_123 matsuura_123 著者名 Matsuura, Yoshiharu
	NRID 1000010759509 1000010759509 著者名 Takayama, Kazuo
	NRID 1000000392308 1000000392308 ORCID 0000-0002-7265-9221 0000-0002-7265-9221 researchmap permalink read0076530 read0076530 Scopus Author ID 55502847200 55502847200 著者名 Yoshioka, Yasuo
キーワード等	Adenoviral vector
	Hypertonic
	Intranasal vaccine
	Resident memory T cells
	Subunit
内容	Hashimoto S., Hirai T., Ueda K., et al. Hypertonic intranasal vaccines gain nasal epithelia access to exert strong immunogenicity. Mucosal Immunology 18, 793 (2025); https://doi.org/10.1016/j.mucimm.2025.03.006.
抄録	Intranasal vaccines potentially offer superior protection against viral infections compared with injectable vaccines. The immunogenicity of intranasal vaccines including adenovirus vector (AdV), has room for improvement, while few options are available for safe execution. In this study, we demonstrate that modifying a basic parameter of vaccine formulation, i.e., osmolarity, can significantly enhance the immunogenicity of intranasal vaccines. Addition of glycerol to AdV intranasal vaccine solutions, unlike other viscous additives, enhanced systemic and mucosal antibodies as well as resident memory T cells in the nasal tissues, which could protect nasal tissue and the lungs against influenza virus. While viscous glycerol could not prolong intranasal retention of solutes, it promoted AdV infection of nasal epithelial cells by facilitating AdV access to the nasal epithelial cell. The enhanced immunogenicity was induced by the hypertonicity of vaccine preparations and sodium chloride, glucose, and mannitol demonstrated the capacity to enhance immunogenicity. Moreover, hypertonic glycerol enhanced the immunogenicity of adjuvanted subunit intranasal vaccines, but not subunit vaccines without adjuvant or injectable vaccines. Overall, the delivery of intranasal vaccines to nasal epithelial cells could be improved through a simple approach, potentially resulting in stronger immunogenicity for certain vaccines.
出版者	Elsevier B.V.
収録物名	Mucosal Immunology
巻(号)	18(4)
ページ	793-809
刊行年月	2025-08-05
言語	英語
ハンドルURL	https://hdl.handle.net/11094/102928
PISSN	19330219
eISSN	19353456
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1016/j.mucimm.2025.03.006
アクセス権	open access
権利情報	© 2025. This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. https://creativecommons.org/licenses/by-nc-nd/4.0/
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Mucosal Immunology. 2025, 18(4), p. 793-809
DC.title	Hypertonic intranasal vaccines gain nasal epithelia access to exert strong immunogenicity
DC.creator	Hashimoto, Soichiro
	Hirai, Toshiro
	Ueda, Koki
	Kakihara, Mako
	Tokunoh, Nagisa
	Ono, Chikako
	Matsuura, Yoshiharu
	Takayama, Kazuo
	Yoshioka, Yasuo
DC.publisher	Elsevier B.V.
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2025-08-05
DC.identifier	https://doi.org/10.1016/j.mucimm.2025.03.006
DC.subject	Adenoviral vector
	Hypertonic
	Intranasal vaccine
	Resident memory T cells
	Subunit
DC.description	Hashimoto S., Hirai T., Ueda K., et al. Hypertonic intranasal vaccines gain nasal epithelia access to exert strong immunogenicity. Mucosal Immunology 18, 793 (2025); https://doi.org/10.1016/j.mucimm.2025.03.006.
DCTERMS.abstract	Intranasal vaccines potentially offer superior protection against viral infections compared with injectable vaccines. The immunogenicity of intranasal vaccines including adenovirus vector (AdV), has room for improvement, while few options are available for safe execution. In this study, we demonstrate that modifying a basic parameter of vaccine formulation, i.e., osmolarity, can significantly enhance the immunogenicity of intranasal vaccines. Addition of glycerol to AdV intranasal vaccine solutions, unlike other viscous additives, enhanced systemic and mucosal antibodies as well as resident memory T cells in the nasal tissues, which could protect nasal tissue and the lungs against influenza virus. While viscous glycerol could not prolong intranasal retention of solutes, it promoted AdV infection of nasal epithelial cells by facilitating AdV access to the nasal epithelial cell. The enhanced immunogenicity was induced by the hypertonicity of vaccine preparations and sodium chloride, glucose, and mannitol demonstrated the capacity to enhance immunogenicity. Moreover, hypertonic glycerol enhanced the immunogenicity of adjuvanted subunit intranasal vaccines, but not subunit vaccines without adjuvant or injectable vaccines. Overall, the delivery of intranasal vaccines to nasal epithelial cells could be improved through a simple approach, potentially resulting in stronger immunogenicity for certain vaccines.
DC.rights	© 2025. This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. https://creativecommons.org/licenses/by-nc-nd/4.0/
DC.format	102928
citation_title	Hypertonic intranasal vaccines gain nasal epithelia access to exert strong immunogenicity
citation_author	Hashimoto, Soichiro
	Hirai, Toshiro
	Ueda, Koki
	Kakihara, Mako
	Tokunoh, Nagisa
	Ono, Chikako
	Matsuura, Yoshiharu
	Takayama, Kazuo
	Yoshioka, Yasuo
citation_publisher	Elsevier B.V.
citation_language	英語
citation_date	2025-08-05
citation_journal_title	Mucosal Immunology
citation_volume	18
citation_issue	4
citation_firstpage	793
citation_lastpage	809
citation_doi	https://doi.org/10.1016/j.mucimm.2025.03.006
citation_public_url	https://hdl.handle.net/11094/102928

Hypertonic intranasal vaccines gain nasal epithelia access to exert strong immunogenicity

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