Mapping NK cell diversity in response to COVID-19 and mRNA vaccination

Kishi, Yosuke; Liu, Yu-Chen; Ishikawa, Masakazu; Yamashita, Maika; Matsumoto, Hisatake; Ogura, Hiroshi; Sakakibara, Shuhei; Okuzaki, Daisuke

doi:https://doi.org/10.1038/S41598-025-11575-W

Mapping NK cell diversity in response to COVID-19 and mRNA vaccination

Kishi, Yosuke; Liu, Yu-Chen; Ishikawa, Masakazu et al.

Scientific Reports, 2025, 15, 37577

アクセス数:57件（2025-12-07 20:27 集計）

固定URL: https://hdl.handle.net/11094/102938

閲覧可能ファイル

ファイル	フォーマット	利用条件	サイズ	閲覧回数	利用開始日	説明	information
SciRep_15_37577	pdf	なし	3.10 MB	17	2025.10.31

Creative Commons : 表示 - 非営利 - 改変禁止

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

タイトル	Mapping NK cell diversity in response to COVID-19 and mRNA vaccination
著者	著者名 Kishi, Yosuke
	NRID 1000050894698 1000050894698 ORCID 0000-0002-2750-0182 0000-0002-2750-0182 researchmap permalink liuyuchen liuyuchen 著者名 Liu, Yu-Chen
	NRID 1000010897028 1000010897028 著者名 Ishikawa, Masakazu
	著者名 Yamashita, Maika
	NRID 1000070644003 1000070644003 ORCID 0000-0001-5771-570X 0000-0001-5771-570X researchmap permalink 38283828 38283828 Scopus Author ID 36632584800 36632584800 著者名 Matsumoto, Hisatake
	NRID 1000070301265 1000070301265 researchmap permalink read0054647 read0054647 Scopus Author ID 7202495132 7202495132 著者名 Ogura, Hiroshi
	NRID 1000010618838 1000010618838 ORCID 0000-0003-3157-5870 0000-0003-3157-5870 researchmap permalink SSakakibara SSakakibara 著者名 Sakakibara, Shuhei
	NRID 1000000346131 1000000346131 ORCID 0000-0002-4552-783X 0000-0002-4552-783X researchmap permalink read0092307 read0092307 Scopus Author ID 6506703054 6506703054 著者名 Okuzaki, Daisuke
キーワード等	NK cells
	COVID-19
	mRNA vaccine
	scRNA-seq
	Trajectory analysis
内容	Kishi Yosuke, Liu Yu-Chen, Ishikawa Masakazu, et al. Mapping NK cell diversity in response to COVID-19 and mRNA vaccination. Scientific Reports 15, 37577 (2025); https://doi.org/10.1038/S41598-025-11575-W.
抄録	Human NK cells exhibit functional heterogeneity, yet the clinical conditions and cellular processes driving this diversity remain insufficiently understood. Here, we report that NK cell diversity emerges during peripheral development, influenced by the divergence of adaptive NK cells from various stages of canonical NK cell maturation during the resolution phase of severe COVID-19. Using scRNA-seq, we analyzed blood NK cells from patients with severe COVID-19 (acute phase and post-intensive care), individuals vaccinated with the SARS-CoV-2 mRNA vaccine (BNT162b2), and healthy donors. The frequencies of immature CD56ᵇʳⁱᵍʰᵗ and proliferating NK cells increased following BNT162b2 vaccination. In contrast, the frequency of adaptive CD56ᵈⁱᵐ cells was markedly elevated in patients recovering from severe COVID-19, alongside clonal expansion and enhanced mitochondrial oxidative phosphorylation. Trajectory analysis revealed a bifurcation in peripheral NK cell development, with CD56ᵇʳⁱᵍʰᵗ cells diverging into canonical and adaptive CD56ᵈⁱᵐ subsets during the course of severe COVID-19. Notably, adaptive NK differentiation exhibited transcriptional and signaling profiles analogous to those of T-cell activation. Thus, NK cell diversity is shaped by the induction of an intrinsic adaptive program. These findings uncover the mechanisms underlying NK cell heterogeneity and have implications for medical applications, including the development of immunotherapies that leverage adaptive NK cell functions.
出版者	Springer Science and Business Media LLC
収録物名	Scientific Reports
巻(号)	15
ページ	37577
刊行年月	2025-10-28
言語	英語
ハンドルURL	https://hdl.handle.net/11094/102938
eISSN	20452322
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1038/S41598-025-11575-W
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Scientific Reports. 2025, 15, p. 37577
DC.title	Mapping NK cell diversity in response to COVID-19 and mRNA vaccination
DC.creator	Kishi, Yosuke
	Liu, Yu-Chen
	Ishikawa, Masakazu
	Yamashita, Maika
	Matsumoto, Hisatake
	Ogura, Hiroshi
	Sakakibara, Shuhei
	Okuzaki, Daisuke
DC.publisher	Springer Science and Business Media LLC
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2025-10-28
DC.identifier	https://doi.org/10.1038/S41598-025-11575-W
DC.subject	NK cells
	COVID-19
	mRNA vaccine
	scRNA-seq
	Trajectory analysis
DC.description	Kishi Yosuke, Liu Yu-Chen, Ishikawa Masakazu, et al. Mapping NK cell diversity in response to COVID-19 and mRNA vaccination. Scientific Reports 15, 37577 (2025); https://doi.org/10.1038/S41598-025-11575-W.
DCTERMS.abstract	Human NK cells exhibit functional heterogeneity, yet the clinical conditions and cellular processes driving this diversity remain insufficiently understood. Here, we report that NK cell diversity emerges during peripheral development, influenced by the divergence of adaptive NK cells from various stages of canonical NK cell maturation during the resolution phase of severe COVID-19. Using scRNA-seq, we analyzed blood NK cells from patients with severe COVID-19 (acute phase and post-intensive care), individuals vaccinated with the SARS-CoV-2 mRNA vaccine (BNT162b2), and healthy donors. The frequencies of immature CD56ᵇʳⁱᵍʰᵗ and proliferating NK cells increased following BNT162b2 vaccination. In contrast, the frequency of adaptive CD56ᵈⁱᵐ cells was markedly elevated in patients recovering from severe COVID-19, alongside clonal expansion and enhanced mitochondrial oxidative phosphorylation. Trajectory analysis revealed a bifurcation in peripheral NK cell development, with CD56ᵇʳⁱᵍʰᵗ cells diverging into canonical and adaptive CD56ᵈⁱᵐ subsets during the course of severe COVID-19. Notably, adaptive NK differentiation exhibited transcriptional and signaling profiles analogous to those of T-cell activation. Thus, NK cell diversity is shaped by the induction of an intrinsic adaptive program. These findings uncover the mechanisms underlying NK cell heterogeneity and have implications for medical applications, including the development of immunotherapies that leverage adaptive NK cell functions.
DC.rights	This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
DC.format	102938
citation_title	Mapping NK cell diversity in response to COVID-19 and mRNA vaccination
citation_author	Kishi, Yosuke
	Liu, Yu-Chen
	Ishikawa, Masakazu
	Yamashita, Maika
	Matsumoto, Hisatake
	Ogura, Hiroshi
	Sakakibara, Shuhei
	Okuzaki, Daisuke
citation_publisher	Springer Science and Business Media LLC
citation_language	英語
citation_date	2025-10-28
citation_journal_title	Scientific Reports
citation_volume	15
citation_firstpage	37577
citation_doi	https://doi.org/10.1038/S41598-025-11575-W
citation_public_url	https://hdl.handle.net/11094/102938

Mapping NK cell diversity in response to COVID-19 and mRNA vaccination

閲覧可能ファイル

論文情報

論文詳細を表示