Effects of a Novel Mammalian-Derived Collagen Matrix on Human Articular Cartilage-Derived Chondrocytes from Osteoarthritis Patients

Wang, Mingyuan; Iwahashi, Toru; Kasuya, Taisuke et al.

International Journal of Molecular Sciences, 2025, 26(16), 7826

アクセス数:199件（2026-02-16 07:33 集計）

固定URL: https://hdl.handle.net/11094/103256

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タイトル
Effects of a Novel Mammalian-Derived Collagen Matrix on Human Articular Cartilage-Derived Chondrocytes from Osteoarthritis Patients
著者
著者名
Wang, Mingyuan
NRID
1000040852108 1000040852108
researchmap permalink
tiwa tiwa
著者名
Iwahashi, Toru
著者名
Kasuya, Taisuke
著者名
Konishi, Mai
著者名
Konishi, Katsuyuki
著者名
Kawanaka, Miki
NRID
1000020512049 1000020512049
researchmap permalink
kanamoto kanamoto
Scopus Author ID
7005012135 7005012135
ORCID
0000-0001-6689-7277 0000-0001-6689-7277
著者名
Kanamoto, Takashi
NRID
1000000432542 1000000432542
ORCID
0000-0001-8622-7422 0000-0001-8622-7422
researchmap permalink
tanahiro-osk tanahiro-osk
Scopus Author ID
55628564591 55628564591
著者名
Tanaka, Hiroyuki
NRID
1000000283747 1000000283747
researchmap permalink
ken-nakata ken-nakata
Scopus Author ID
7402151308 7402151308
著者名
Nakata, Ken
キーワード等
autologous chondrocyte implantation
chondrocytes
collagen
osteoarthritis
proteoglycans
内容
Wang M., Iwahashi T., Kasuya T., et al. Effects of a Novel Mammalian-Derived Collagen Matrix on Human Articular Cartilage-Derived Chondrocytes from Osteoarthritis Patients. International Journal of Molecular Sciences 26, 7826 (2025); https://doi.org/10.3390/ijms26167826.
抄録
Osteoarthritis (OA) is the most common joint disorder worldwide. Autologous chondrocyte implantation (ACI) is an established treatment for articular cartilage defects of the knee, but its effectiveness in OA is still under investigation. In this study, we investigated the effects of a newly developed mammalian-derived collagen matrix, NC-Col, on the proliferation, migration, adhesion, and gene expression of human articular cartilage-derived chondrocytes from OA patients in vitro, using proliferation assays, wound healing assays, adhesion assays, RT-qPCR, and RNA sequencing, respectively. In addition, the effects of NC-Col were compared with three different commercially available collagen matrices, and the underlying molecular mechanisms through which NC-Col influences these cellular behaviours were explored. Our results showed that NC-Col, used as a coating matrix, enhances cell proliferation, maintains the phenotype, and upregulates Proteoglycan 4 (PRG4) in human articular cartilage-derived chondrocytes. Inhibition of the PI3K-Akt signalling pathway was found to be involved in some of these effects. In conclusion, our findings suggest that NC-Col collagen may offer new strategies for improving therapeutic outcomes in OA, particularly in the context of ACI.
出版者
Multidisciplinary Digital Publishing Institute (MDPI)
収録物名
International Journal of Molecular Sciences
巻(号)
26(16)
ページ
7826
刊行年月
2025-08-13
言語
英語
ハンドルURL
https://hdl.handle.net/11094/103256
PISSN
16616596
eISSN
14220067
関連情報 (isIdenticalTo)
DOI (出版社版)
https://doi.org/10.3390/ijms26167826
アクセス権
open access
権利情報
This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ
Version of Record
カテゴリ
学術雑誌論文 Journal Article