Development of starch-based support material alternately extruded with gelatin-based bioinks for 3D bioprinting application

Prasetyaningrum, Pekik Wiji; Mubarok, Wildan; Kotani, Takashi; Sakai, Shinji

doi:https://doi.org/10.1016/j.bprint.2025.e00439

Development of starch-based support material alternately extruded with gelatin-based bioinks for 3D bioprinting application

Prasetyaningrum, Pekik Wiji; Mubarok, Wildan; Kotani, Takashi et al.

Bioprinting, 2025, 51, e00439

アクセス数:194件（2026-02-14 06:33 集計）

固定URL: https://hdl.handle.net/11094/103286

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タイトル	Development of starch-based support material alternately extruded with gelatin-based bioinks for 3D bioprinting application
著者	著者名 Prasetyaningrum, Pekik Wiji
	NRID 1000080975433 1000080975433 ORCID 0000-0003-2545-1206 0000-0003-2545-1206 researchmap permalink wildanmubarok wildanmubarok Scopus Author ID 57208440063 57208440063 著者名 Mubarok, Wildan
	著者名 Kotani, Takashi
	NRID 1000020359938 1000020359938 ORCID 0000-0002-1041-4798 0000-0002-1041-4798 researchmap permalink sakaishinji sakaishinji Scopus Author ID 35450082900 35450082900 著者名 Sakai, Shinji
キーワード等	3D bioprinting
	Gelatin
	Horseradish peroxidase
	Starch
	Stem cells
内容	Prasetyaningrum P.W., Mubarok W., Kotani T., et al. Development of starch-based support material alternately extruded with gelatin-based bioinks for 3D bioprinting application. Bioprinting 51, e00439 (2025); https://doi.org/10.1016/j.bprint.2025.e00439.
抄録	The use of support materials is crucial for the 3D bioprinting of low-viscosity bioinks, which yield soft hydrogel constructs susceptible to deformation under their weight. In this study, we developed a starch-based support material that provides structural support during printing and supplies hydrogen peroxide (H₂O₂), for printing cell-laden constructs from low-viscosity bioinks (4.4–53.1 mPa s at 1 s⁻¹ shear rate) composed of a gelatin derivative possessing phenolic hydroxyl moieties (gelatin-Ph), horseradish peroxidase (HRP), and cells. Importantly, the support material can be selectively and gently removed using α-amylase, a biocompatible enzyme, without harming the construct or encapsulated cells, which is a significant advancement over conventional methods of removing support systems. 3D constructs were fabricated by alternately extruding bioinks containing 5.0 w/v% gelatin-Ph and 10 U/mL HRP with a support material consisting of 16.7 w/w% starch and 10 mM H₂O₂. Immortalized human bone marrow-derived mesenchymal stem cells encapsulated within the constructs showed >80 % viability after printing and exhibited an elongated morphology and proliferation, while maintaining their stemness over 14 days of culture. The cells underwent osteogenic differentiation when cultured in a differentiation medium, as evidenced by the calcium deposition, alkaline phosphatase activity, and expression of osteogenic genes, demonstrating the potential of the proposed approach for tissue-engineering applications.
出版者	Elsevier B.V.
収録物名	Bioprinting
巻(号)	51
ページ	e00439
刊行年月	2025-09-20
言語	英語
ハンドルURL	https://hdl.handle.net/11094/103286
PISSN	24058866
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1016/j.bprint.2025.e00439
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Bioprinting. 2025, 51, p. e00439
DC.title	Development of starch-based support material alternately extruded with gelatin-based bioinks for 3D bioprinting application
DC.creator	Prasetyaningrum, Pekik Wiji
	Mubarok, Wildan
	Kotani, Takashi
	Sakai, Shinji
DC.publisher	Elsevier B.V.
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2025-09-20
DC.identifier	https://doi.org/10.1016/j.bprint.2025.e00439
DC.subject	3D bioprinting
	Gelatin
	Horseradish peroxidase
	Starch
	Stem cells
DC.description	Prasetyaningrum P.W., Mubarok W., Kotani T., et al. Development of starch-based support material alternately extruded with gelatin-based bioinks for 3D bioprinting application. Bioprinting 51, e00439 (2025); https://doi.org/10.1016/j.bprint.2025.e00439.
DCTERMS.abstract	The use of support materials is crucial for the 3D bioprinting of low-viscosity bioinks, which yield soft hydrogel constructs susceptible to deformation under their weight. In this study, we developed a starch-based support material that provides structural support during printing and supplies hydrogen peroxide (H₂O₂), for printing cell-laden constructs from low-viscosity bioinks (4.4–53.1 mPa s at 1 s⁻¹ shear rate) composed of a gelatin derivative possessing phenolic hydroxyl moieties (gelatin-Ph), horseradish peroxidase (HRP), and cells. Importantly, the support material can be selectively and gently removed using α-amylase, a biocompatible enzyme, without harming the construct or encapsulated cells, which is a significant advancement over conventional methods of removing support systems. 3D constructs were fabricated by alternately extruding bioinks containing 5.0 w/v% gelatin-Ph and 10 U/mL HRP with a support material consisting of 16.7 w/w% starch and 10 mM H₂O₂. Immortalized human bone marrow-derived mesenchymal stem cells encapsulated within the constructs showed >80 % viability after printing and exhibited an elongated morphology and proliferation, while maintaining their stemness over 14 days of culture. The cells underwent osteogenic differentiation when cultured in a differentiation medium, as evidenced by the calcium deposition, alkaline phosphatase activity, and expression of osteogenic genes, demonstrating the potential of the proposed approach for tissue-engineering applications.
DC.rights	This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
DC.format	103286
citation_title	Development of starch-based support material alternately extruded with gelatin-based bioinks for 3D bioprinting application
citation_author	Prasetyaningrum, Pekik Wiji
	Mubarok, Wildan
	Kotani, Takashi
	Sakai, Shinji
citation_publisher	Elsevier B.V.
citation_language	英語
citation_date	2025-09-20
citation_journal_title	Bioprinting
citation_volume	51
citation_firstpage	e00439
citation_doi	https://doi.org/10.1016/j.bprint.2025.e00439
citation_public_url	https://hdl.handle.net/11094/103286

Development of starch-based support material alternately extruded with gelatin-based bioinks for 3D bioprinting application

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