Using saturated absorption for superresolution laser scanning transmission microscopy

Nishida, Kentaro; Sato, Hikaru; Oketani, Ryosuke et al.

Journal of Microscopy, 2022, 288(2), 117-129

アクセス数:190件（2026-02-16 23:05 集計）

固定URL: https://hdl.handle.net/11094/103306

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論文情報

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タイトル
Using saturated absorption for superresolution laser scanning transmission microscopy
著者
著者名
Nishida, Kentaro
著者名
Sato, Hikaru
NRID
1000000908890 1000000908890
著者名
Oketani, Ryosuke
NRID
1000050868768 1000050868768
著者名
Mochizuki, Kentaro
NRID
1000070993533 1000070993533
ORCID
0000-0002-9968-3459 0000-0002-9968-3459
researchmap permalink
k_temma k_temma
著者名
Temma, Kenta
NRID
1000030611727 1000030611727
ORCID
0000-0002-4398-288X 0000-0002-4398-288X
researchmap permalink
jemappellekuma jemappellekuma
Scopus Author ID
24376935000 24376935000
著者名
Kumamoto, Yasuaki
NRID
1000060236619 1000060236619
著者名
Tanaka, Hideo
NRID
1000080362664 1000080362664
ORCID
0000-0002-2284-375X 0000-0002-2284-375X
researchmap permalink
read0089911 read0089911
Scopus Author ID
7404058829 7404058829
著者名
Fujita, Katsumasa
キーワード等
nonlinear microscopy
optical sectioning
saturable absorption
superresolution microscopy
tissue imaging
transmission microscopy
内容
This is the peer reviewed version of the following article: Nishida K., Sato H., Oketani R., et al. Using saturated absorption for superresolution laser scanning transmission microscopy. Journal of Microscopy 288, 117 (2022), which has been published in final form at https://doi.org/10.1111/jmi.13033. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.
抄録
We improved the three-dimensional spatial resolution of laser scanning transmission microscopy by exploiting the saturated absorption of dye molecules. The saturated absorption is induced by the high-intensity light irradiation and localises the signal within the centre of the focal spot. Our numerical calculation indicates that the spatial resolution in transmission imaging is significantly improved for both lateral and axial directions using nonlinear transmitted signals induced by saturated absorption. We experimentally demonstrated the improvement of the three-dimensional resolution by observing fine structures of stained rat kidney tissues, which were not able to be visualised by conventional laser scanning transmission microscopy.
出版者
John Wiley and Sons Inc
収録物名
Journal of Microscopy
巻(号)
288(2)
ページ
117-129
刊行年月
2022-11-21
言語
英語
ハンドルURL
https://hdl.handle.net/11094/103306
PISSN
00222720
eISSN
13652818
関連情報 (isVersionOf)
DOI (出版社版)
https://doi.org/10.1111/jmi.13033
アクセス権
open access
権利情報
© 2021 Royal Microscopical Society.
出版タイプ
Accepted Manuscript
カテゴリ
学術雑誌論文 Journal Article