学術雑誌論文

学術雑誌論文

text

Optics Letters. 2020, 46(1), p. 37-40

Hyperspectral two-photon excitation microscopy using visible wavelength

The Optical Society

英語

text

2020-12-18

https://doi.org/10.1364/OL.413526

Kubo T., Temma K., Smith N.I., et al. Hyperspectral two-photon excitation microscopy using visible wavelength. Optics Letters 46, 37 (2021); https://doi.org/10.1364/OL.413526.

We demonstrate hyperspectral imaging by visible-wavelength two-photon excitation microscopy using line illumination and slit-confocal detection. A femtosecond pulsed laser light at 530 nm was used for the simultaneous excitation of fluorescent proteins with different emission wavelengths. The use of line illumination enabled efficient detection of hyperspectral images and achieved simultaneous detection of three fluorescence spectra in the observation of living HeLa cells with an exposure time of 1 ms per line, which is equivalent to about 2 µs per pixel in point scanning, with 160 data points per spectrum. On combining linear spectral unmixing techniques, localization of fluorescent probes in the cells was achieved. A theoretical investigation of the imaging property revealed high-depth discrimination property attained through the combination of nonlinear excitation and slit detection.

© Optical Society of America 2021 Optica Publishing Group. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modifications of the content of this paper are prohibited.

103309

Hyperspectral two-photon excitation microscopy using visible wavelength

The Optical Society

英語

2020-12-18

Optics Letters

46

1

37

40

https://doi.org/10.1364/OL.413526

https://hdl.handle.net/11094/103309