学術雑誌論文

学術雑誌論文

text

Analyst. 2019, 144(8), p. 2531-2540

Using redox-sensitive mitochondrial cytochrome Raman bands for label-free detection of mitochondrial dysfunction

Royal Society of Chemistry

英語

text

2019-02-15

https://doi.org/10.1039/c8an02213e

Reproduced from Analyst 144, 2531 (2019) with permission from the Royal Society of Chemistry.

Mitochondrial activity is a widely used criterion to judge the metabolic condition of a living specimen. Numerous methods have been developed for related analyses, including the detection of O₂ consumption, trans-membrane potential, and ATP production. In this study, we demonstrate that the redox state of cytochromes can serve as a sensitive mitochondrial activity indicator in glutamate-stressed neuronal cells. Mitochondrial dysfunction was detected by Raman imaging as early as 30 min after glutamate-stress induction. By comparing this result with other commonly used mitochondrial function assays, we found Raman imaging has a similar sensitivity to ATP production and trans-membrane potential assays. Other viability tests, such as MTT assay and ROS production tests, showed a slower response than our method. A thorough understanding of cytochrome dynamics with our new method will help establish Raman spectroscopy as a competitive clinical diagnosis tool for neurodegenerative diseases involving mitochondrial dysfunction.

103313

Using redox-sensitive mitochondrial cytochrome Raman bands for label-free detection of mitochondrial dysfunction

Royal Society of Chemistry

英語

2019-02-15

Analyst

144

8

2531

2540

https://doi.org/10.1039/c8an02213e

https://hdl.handle.net/11094/103313