Aerobic degradation characteristics of cis-1,2-dichloroethene by Pseudonocardia sp. D17: Degradation kinetics, putative degradation pathways, and involvement of soluble di-iron monooxygenases in the initial oxidation

Nishimine, Ryugo; Kaneko, Yuna; Fujiwara, Shinpei; Inoue, Daisuke; Takeo, Masahiro; Ike, Michihiko

doi:https://doi.org/10.1016/j.jbiosc.2025.10.012

Aerobic degradation characteristics of cis-1,2-dichloroethene by Pseudonocardia sp. D17: Degradation kinetics, putative degradation pathways, and involvement of soluble di-iron monooxygenases in the initial oxidation

Nishimine, Ryugo; Kaneko, Yuna; Fujiwara, Shinpei et al.

Journal of Bioscience and Bioengineering, 2025

アクセス数:196件（2026-02-16 01:17 集計）

固定URL: https://hdl.handle.net/11094/103324

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タイトル	Aerobic degradation characteristics of cis-1,2-dichloroethene by Pseudonocardia sp. D17: Degradation kinetics, putative degradation pathways, and involvement of soluble di-iron monooxygenases in the initial oxidation
著者	著者名 Nishimine, Ryugo
	著者名 Kaneko, Yuna
	著者名 Fujiwara, Shinpei
	NRID 1000070448091 1000070448091 ORCID 0000-0002-9216-8198 0000-0002-9216-8198 researchmap permalink DaisukeINOUE DaisukeINOUE Scopus Author ID 55723196300 55723196300 著者名 Inoue, Daisuke
	NRID 1000040236443 1000040236443 著者名 Takeo, Masahiro
	NRID 1000040222856 1000040222856 ORCID 0000-0001-6237-8103 0000-0001-6237-8103 researchmap permalink ikemichi ikemichi Scopus Author ID 55551254800 55551254800 著者名 Ike, Michihiko
キーワード等	Biodegradation
	cis-1,2-Dichloroethene
	Degradation pathways
	Pseudonocardia sp. D17
	Soluble di-iron monooxygenase
内容	Nishimine R., Kaneko Y., Fujiwara S., et al. Aerobic degradation characteristics of cis-1,2-dichloroethene by Pseudonocardia sp. D17: Degradation kinetics, putative degradation pathways, and involvement of soluble di-iron monooxygenases in the initial oxidation. Journal of Bioscience and Bioengineering , (2025); https://doi.org/10.1016/j.jbiosc.2025.10.012.
抄録	Pseudonocardia sp. D17 (D17) is a novel strain capable of aerobically metabolizing cis-1,2-dichloroethene (cDCE), a persistent contaminant in soil and groundwater. This study aimed to investigate the cDCE degradation characteristics of D17 with respect to kinetics, associated degradative enzymes, and degradation pathways. Degradation experiments with cDCE concentrations ranging from 0.267 to 91.3 μM revealed that D17 can efficiently degrade cDCE across this range. The maximum specific degradation rate and half saturation constant for cDCE degradation by D17 were estimated to be 0.418 ± 0.045 nmol/mg-protein/min and 38.5 ± 9.2 μM, respectively. Heterologous expression experiments demonstrated that both group 5 soluble di-iron monooxygenases in D17, namely tetrahydrofuran and propane monooxygenases, can catalyze cDCE degradation with higher catalytic activity observed in the former. This suggests their involvement in cDCE degradation by D17. It was also proposed that D17 completely dechlorinates cDCE through multiple pathways to generate glyoxylic acid, which is either mineralized or incorporated into the glyoxylate cycle, with a minor portion being converted to oxalic acid as a dead-end product. These findings provide novel insights into metabolic aerobic cDCE biodegradation and highlight the potential of D17 as a bioremediation agent.
出版者	Elsevier B.V.
収録物名	Journal of Bioscience and Bioengineering
刊行年月	2025-11-12
言語	英語
ハンドルURL	https://hdl.handle.net/11094/103324
PISSN	13891723
eISSN	13474421
関連情報 (isVersionOf)	DOI (出版社版) https://doi.org/10.1016/j.jbiosc.2025.10.012
アクセス権	embargoed access
権利情報	© 2025. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/
権利情報	The full-text file will be made open to the public on 12 November 2026 in accordance with the publisher's policy.
出版タイプ	Accepted Manuscript
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Journal of Bioscience and Bioengineering. 2025
DC.title	Aerobic degradation characteristics of cis-1,2-dichloroethene by Pseudonocardia sp. D17: Degradation kinetics, putative degradation pathways, and involvement of soluble di-iron monooxygenases in the initial oxidation
DC.creator	Nishimine, Ryugo
	Kaneko, Yuna
	Fujiwara, Shinpei
	Inoue, Daisuke
	Takeo, Masahiro
	Ike, Michihiko
DC.publisher	Elsevier B.V.
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2025-11-12
DC.identifier	https://doi.org/10.1016/j.jbiosc.2025.10.012
DC.subject	Biodegradation
	cis-1,2-Dichloroethene
	Degradation pathways
	Pseudonocardia sp. D17
	Soluble di-iron monooxygenase
DC.description	Nishimine R., Kaneko Y., Fujiwara S., et al. Aerobic degradation characteristics of cis-1,2-dichloroethene by Pseudonocardia sp. D17: Degradation kinetics, putative degradation pathways, and involvement of soluble di-iron monooxygenases in the initial oxidation. Journal of Bioscience and Bioengineering , (2025); https://doi.org/10.1016/j.jbiosc.2025.10.012.
DCTERMS.abstract	Pseudonocardia sp. D17 (D17) is a novel strain capable of aerobically metabolizing cis-1,2-dichloroethene (cDCE), a persistent contaminant in soil and groundwater. This study aimed to investigate the cDCE degradation characteristics of D17 with respect to kinetics, associated degradative enzymes, and degradation pathways. Degradation experiments with cDCE concentrations ranging from 0.267 to 91.3 μM revealed that D17 can efficiently degrade cDCE across this range. The maximum specific degradation rate and half saturation constant for cDCE degradation by D17 were estimated to be 0.418 ± 0.045 nmol/mg-protein/min and 38.5 ± 9.2 μM, respectively. Heterologous expression experiments demonstrated that both group 5 soluble di-iron monooxygenases in D17, namely tetrahydrofuran and propane monooxygenases, can catalyze cDCE degradation with higher catalytic activity observed in the former. This suggests their involvement in cDCE degradation by D17. It was also proposed that D17 completely dechlorinates cDCE through multiple pathways to generate glyoxylic acid, which is either mineralized or incorporated into the glyoxylate cycle, with a minor portion being converted to oxalic acid as a dead-end product. These findings provide novel insights into metabolic aerobic cDCE biodegradation and highlight the potential of D17 as a bioremediation agent.
DC.rights	© 2025. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/
DC.rights	The full-text file will be made open to the public on 12 November 2026 in accordance with the publisher's policy.
DC.format	103324
citation_title	Aerobic degradation characteristics of cis-1,2-dichloroethene by Pseudonocardia sp. D17: Degradation kinetics, putative degradation pathways, and involvement of soluble di-iron monooxygenases in the initial oxidation
citation_author	Nishimine, Ryugo
	Kaneko, Yuna
	Fujiwara, Shinpei
	Inoue, Daisuke
	Takeo, Masahiro
	Ike, Michihiko
citation_publisher	Elsevier B.V.
citation_language	英語
citation_date	2025-11-12
citation_journal_title	Journal of Bioscience and Bioengineering
citation_doi	https://doi.org/10.1016/j.jbiosc.2025.10.012
citation_public_url	https://hdl.handle.net/11094/103324

Aerobic degradation characteristics of cis-1,2-dichloroethene by Pseudonocardia sp. D17: Degradation kinetics, putative degradation pathways, and involvement of soluble di-iron monooxygenases in the initial oxidation

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