High-throughput line-illumination Raman microscopy with multislit detection

Mochizuki, Kentaro; Kumamoto, Yasuaki; Maeda, Shunsuke; Tanuma, Masato; Kasai, Atsushi; Takemura, Masashi; Harada, Yoshinori; Hashimoto, Hitoshi; Tanaka, Hideo; Smith, Nicholas Isaac; Fujita, Katsumasa

doi:https://doi.org/10.1364/BOE.480611

High-throughput line-illumination Raman microscopy with multislit detection

Mochizuki, Kentaro; Kumamoto, Yasuaki; Maeda, Shunsuke et al.

Biomedical Optics Express, 2023, 14(3), 1015-1026

アクセス数:260件（2026-02-16 02:10 集計）

固定URL: https://hdl.handle.net/11094/103326

閲覧可能ファイル

ファイル	フォーマット	利用条件	サイズ	閲覧回数	利用開始日	説明	information
BiomedOptExpress_14_3_1015	pdf	なし	6.15 MB	24	2025.12.05

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

タイトル	High-throughput line-illumination Raman microscopy with multislit detection
著者	NRID 1000050868768 1000050868768 著者名 Mochizuki, Kentaro
	NRID 1000030611727 1000030611727 ORCID 0000-0002-4398-288X 0000-0002-4398-288X researchmap permalink jemappellekuma jemappellekuma Scopus Author ID 24376935000 24376935000 著者名 Kumamoto, Yasuaki
	著者名 Maeda, Shunsuke
	著者名 Tanuma, Masato
	NRID 1000040454649 1000040454649 著者名 Kasai, Atsushi
	著者名 Takemura, Masashi
	NRID 1000010381956 1000010381956 著者名 Harada, Yoshinori
	NRID 1000030240849 1000030240849 ORCID 0000-0001-6548-4016 0000-0001-6548-4016 researchmap permalink hitoshi-hashimoto hitoshi-hashimoto Scopus Author ID 35352982100 35352982100 著者名 Hashimoto, Hitoshi
	NRID 1000060236619 1000060236619 著者名 Tanaka, Hideo
	NRID 1000010362592 1000010362592 ORCID 0000-0002-7835-199X 0000-0002-7835-199X researchmap permalink read0089935 read0089935 Scopus Author ID 55262815400 55262815400 著者名 Smith, Nicholas Isaac
	NRID 1000080362664 1000080362664 ORCID 0000-0002-2284-375X 0000-0002-2284-375X researchmap permalink read0089911 read0089911 Scopus Author ID 7404058829 7404058829 著者名 Fujita, Katsumasa
内容	Mochizuki K., Kumamoto Y., Maeda S., et al. High-throughput line-illumination Raman microscopy with multislit detection. Biomedical Optics Express 14, 1015 (2023); https://doi.org/10.1364/BOE.480611.
抄録	Raman microscopy is an emerging tool for molecular imaging and analysis of living samples. Use of Raman microscopy in life sciences is, however, still limited because of its slow measurement speed for spectral imaging and analysis. We developed a multiline-illumination Raman microscope to achieve ultrafast Raman spectral imaging. A spectrophotometer equipped with a periodic array of confocal slits detects Raman spectra from a sample irradiated by multiple line illuminations. A comb-like Raman hyperspectral image is formed on a two-dimensional detector in the spectrophotometer, and a hyperspectral Raman image is acquired by scanning the sample with multiline illumination array. By irradiating a sample with 21 simultaneous illumination lines, we achieved high-throughput Raman hyperspectral imaging of mouse brain tissue, acquiring 1108800 spectra in 11.4 min. We also measured mouse kidney and liver tissue as well as conducted label-free live-cell molecular imaging. The ultrafast Raman hyperspectral imaging enabled by the presented technique will expand the possible applications of Raman microscopy in biological and medical fields.
出版者	Optica Publishing Group (formerly OSA)
収録物名	Biomedical Optics Express
巻(号)	14(3)
ページ	1015-1026
刊行年月	2023-02-07
言語	英語
ハンドルURL	https://hdl.handle.net/11094/103326
eISSN	21567085
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1364/BOE.480611
アクセス権	open access
権利情報	© 2023 Optica Publishing Group. Users may use, reuse, and build upon the article, or use the article for text or data mining, so long as such uses are for non-commercial purposes and appropriate attribution is maintained. All other rights are reserved.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Biomedical Optics Express. 2023, 14(3), p. 1015-1026
DC.title	High-throughput line-illumination Raman microscopy with multislit detection
DC.creator	Mochizuki, Kentaro
	Kumamoto, Yasuaki
	Maeda, Shunsuke
	Tanuma, Masato
	Kasai, Atsushi
	Takemura, Masashi
	Harada, Yoshinori
	Hashimoto, Hitoshi
	Tanaka, Hideo
	Smith, Nicholas Isaac
	Fujita, Katsumasa
DC.publisher	Optica Publishing Group (formerly OSA)
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2023-02-07
DC.identifier	https://doi.org/10.1364/BOE.480611
DC.description	Mochizuki K., Kumamoto Y., Maeda S., et al. High-throughput line-illumination Raman microscopy with multislit detection. Biomedical Optics Express 14, 1015 (2023); https://doi.org/10.1364/BOE.480611.
DCTERMS.abstract	Raman microscopy is an emerging tool for molecular imaging and analysis of living samples. Use of Raman microscopy in life sciences is, however, still limited because of its slow measurement speed for spectral imaging and analysis. We developed a multiline-illumination Raman microscope to achieve ultrafast Raman spectral imaging. A spectrophotometer equipped with a periodic array of confocal slits detects Raman spectra from a sample irradiated by multiple line illuminations. A comb-like Raman hyperspectral image is formed on a two-dimensional detector in the spectrophotometer, and a hyperspectral Raman image is acquired by scanning the sample with multiline illumination array. By irradiating a sample with 21 simultaneous illumination lines, we achieved high-throughput Raman hyperspectral imaging of mouse brain tissue, acquiring 1108800 spectra in 11.4 min. We also measured mouse kidney and liver tissue as well as conducted label-free live-cell molecular imaging. The ultrafast Raman hyperspectral imaging enabled by the presented technique will expand the possible applications of Raman microscopy in biological and medical fields.
DC.rights	© 2023 Optica Publishing Group. Users may use, reuse, and build upon the article, or use the article for text or data mining, so long as such uses are for non-commercial purposes and appropriate attribution is maintained. All other rights are reserved.
DC.format	103326
citation_title	High-throughput line-illumination Raman microscopy with multislit detection
citation_author	Mochizuki, Kentaro
	Kumamoto, Yasuaki
	Maeda, Shunsuke
	Tanuma, Masato
	Kasai, Atsushi
	Takemura, Masashi
	Harada, Yoshinori
	Hashimoto, Hitoshi
	Tanaka, Hideo
	Smith, Nicholas Isaac
	Fujita, Katsumasa
citation_publisher	Optica Publishing Group (formerly OSA)
citation_language	英語
citation_date	2023-02-07
citation_journal_title	Biomedical Optics Express
citation_volume	14
citation_issue	3
citation_firstpage	1015
citation_lastpage	1026
citation_doi	https://doi.org/10.1364/BOE.480611
citation_public_url	https://hdl.handle.net/11094/103326

High-throughput line-illumination Raman microscopy with multislit detection

閲覧可能ファイル

論文情報

論文詳細を表示