学術雑誌論文

学術雑誌論文

text

Biomedical Optics Express. 2022, 13(2), p. 995-1004

Spectral focusing in picosecond pulsed stimulated Raman scattering microscopy

The Optical Society

英語

text

2022-01-27

https://doi.org/10.1364/BOE.445640

Koike K., Smith N.I., Fujita K.. Spectral focusing in picosecond pulsed stimulated Raman scattering microscopy. Biomedical Optics Express 13, 995 (2022); https://doi.org/10.1364/BOE.445640.

We introduce spectral focusing of picosecond laser pulses in stimulated Raman scattering (SRS) microscopy to improve spectral resolution, reduce nonlinear background signals, and decrease nonlinear photodamage. We produce a pair of 14 ps pump and Stokes laser pulses by spectral focusing of a 2 ps laser and achieve a spectral resolution of 2 cm⁻¹. Due to instantaneous narrow-band excitation, we find that the chirped 14 ps laser pulses can be used to improve the signal-to-background ratio in SRS microscopy of various samples such as polymer particles and small molecules in HeLa cells. The lower peak powers produced by chirped picosecond laser pulses also reduce nonlinear photodamage, allowing long-term SRS imaging of living cells with higher SNR.

© 2022 Optica Publishing Group. Users may use, reuse, and build upon the article, or use the article for text or data mining, so long as such uses are for non-commercial purposes and appropriate attribution is maintained. All other rights are reserved.

103329

Spectral focusing in picosecond pulsed stimulated Raman scattering microscopy

The Optical Society

英語

2022-01-27

Biomedical Optics Express

13

2

995

1004

https://doi.org/10.1364/BOE.445640

https://hdl.handle.net/11094/103329