Bessel-beam illumination Raman microscopy

Bando, Kazuki; Yabuuchi, Shumpei; Li, Menglu et al.

Biomedical Optics Express, 2022, 13(6), 3161-3170

アクセス数:215件（2026-02-15 21:36 集計）

固定URL: https://hdl.handle.net/11094/103330

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タイトル
Bessel-beam illumination Raman microscopy
著者
NRID
1000040795952 1000040795952
researchmap permalink
kazukibando kazukibando
Scopus Author ID
11540437400 11540437400
著者名
Bando, Kazuki
著者名
Yabuuchi, Shumpei
著者名
Li, Menglu
NRID
1000050975159 1000050975159
ORCID
0000-0002-5696-4111 0000-0002-5696-4111
researchmap permalink
kubo_toshiki_8 kubo_toshiki_8
著者名
Kubo, Toshiki
NRID
1000000908890 1000000908890
著者名
Oketani, Ryosuke
NRID
1000010362592 1000010362592
ORCID
0000-0002-7835-199X 0000-0002-7835-199X
researchmap permalink
read0089935 read0089935
Scopus Author ID
55262815400 55262815400
著者名
Smith, Nicholas I.
NRID
1000080362664 1000080362664
ORCID
0000-0002-2284-375X 0000-0002-2284-375X
researchmap permalink
read0089911 read0089911
Scopus Author ID
7404058829 7404058829
著者名
Fujita, Katsumasa
内容
Bando K., Yabuuchi S., Li M., et al. Bessel-beam illumination Raman microscopy. Biomedical Optics Express 13, 3161 (2022); https://doi.org/10.1364/BOE.456138.
抄録
We demonstrate the use of Bessel beams for side illumination slit-scanning Raman imaging for label-free and hyperspectral analysis of cell spheroids. The background elimination by the side illumination and the aberration-resistant Bessel beam drastically improves the image contrast in Raman observation, allowing label-free investigation of intracellular molecules in thick biological samples. Live cell spheroids were observed to confirm the improvement in image contrast and background reduction with Bessel illumination compared to conventional epi-line illumination.
出版者
Optica Publishing Group (formerly OSA)
収録物名
Biomedical Optics Express
巻(号)
13(6)
ページ
3161-3170
刊行年月
2022-05-03
言語
英語
ハンドルURL
https://hdl.handle.net/11094/103330
eISSN
21567085
関連情報 (isIdenticalTo)
DOI (出版社版)
https://doi.org/10.1364/BOE.456138
アクセス権
open access
権利情報
© 2022 Optica Publishing Group. Users may use, reuse, and build upon the article, or use the article for text or data mining, so long as such uses are for non-commercial purposes and appropriate attribution is maintained. All other rights are reserved.
出版タイプ
Version of Record
カテゴリ
学術雑誌論文 Journal Article