Saturated-excitation image scanning microscopy

Temma, Kenta; Oketani, Ryosuke; Lachmann, René et al.

Optics Express, 2022, 30(8), 13825-13838

アクセス数:205件（2026-02-16 04:11 集計）

固定URL: https://hdl.handle.net/11094/103331

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論文情報

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タイトル
Saturated-excitation image scanning microscopy
著者
NRID
1000070993533 1000070993533
ORCID
0000-0002-9968-3459 0000-0002-9968-3459
researchmap permalink
k_temma k_temma
著者名
Temma, Kenta
NRID
1000000908890 1000000908890
著者名
Oketani, Ryosuke
著者名
Lachmann, René
NRID
1000050975159 1000050975159
ORCID
0000-0002-5696-4111 0000-0002-5696-4111
researchmap permalink
kubo_toshiki_8 kubo_toshiki_8
著者名
Kubo, Toshiki
NRID
1000010362592 1000010362592
ORCID
0000-0002-7835-199X 0000-0002-7835-199X
researchmap permalink
read0089935 read0089935
Scopus Author ID
55262815400 55262815400
著者名
Smith, Nicholas I.
著者名
Heintzmann, Rainer
NRID
1000080362664 1000080362664
ORCID
0000-0002-2284-375X 0000-0002-2284-375X
researchmap permalink
read0089911 read0089911
Scopus Author ID
7404058829 7404058829
著者名
Fujita, Katsumasa
内容
Temma K., Oketani R., Lachmann R., et al. Saturated-excitation image scanning microscopy. Optics Express 30, 13825 (2022); https://doi.org/10.1364/OE.455621.
抄録
Image scanning microscopy (ISM) overcomes the trade-off between spatial resolution and signal volume in confocal microscopy by rearranging the signal distribution on a two-dimensional detector array to achieve a spatial resolution close to the theoretical limit achievable by infinitesimal pinhole detection without sacrificing the detected signal intensity. In this paper, we improved the spatial resolution of ISM in three dimensions by exploiting saturated excitation (SAX) of fluorescence. We theoretically investigated the imaging properties of ISM, when the fluorescence signals are nonlinearly induced by SAX, and show combined SAX-ISM fluorescence imaging to demonstrate the improvement of the spatial resolution in three dimensions. In addition, we confirmed that the SNR of SAX-ISM imaging of fluorescent beads and biological samples, which is one of the challenges in conventional SAX microscopy, was improved.
出版者
Optica Publishing Group (formerly OSA)
収録物名
Optics Express
巻(号)
30(8)
ページ
13825-13838
刊行年月
2022-04-08
言語
英語
ハンドルURL
https://hdl.handle.net/11094/103331
eISSN
10944087
関連情報 (isIdenticalTo)
DOI (出版社版)
https://doi.org/10.1364/OE.455621
アクセス権
open access
権利情報
© 2022 Optica Publishing Group. Users may use, reuse, and build upon the article, or use the article for text or data mining, so long as such uses are for non-commercial purposes and appropriate attribution is maintained. All other rights are reserved.
出版タイプ
Version of Record
カテゴリ
学術雑誌論文 Journal Article