Deficiencies in methionine, tryptophan, and niacin remodels intestinal transcriptome and gut microbiota in female mice

Hara, Tomoaki; Meng, Sikun; Motooka, Daisuke; Arao, Yasuko; Saito, Yoshiko; Rennie, Sarah; Uchida, Shizuka; Ofusa, Ken; Arai, Takahiro; Konno, Masamitsu; Satoh, Taroh; Ishii, Hideshi

doi:https://doi.org/10.1038/s41598-025-18046-2

Deficiencies in methionine, tryptophan, and niacin remodels intestinal transcriptome and gut microbiota in female mice

Hara, Tomoaki; Meng, Sikun; Motooka, Daisuke et al.

Scientific Reports, 2025, 15, 36155

アクセス数:179件（2026-02-16 10:17 集計）

固定URL: https://hdl.handle.net/11094/103486

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タイトル	Deficiencies in methionine, tryptophan, and niacin remodels intestinal transcriptome and gut microbiota in female mice
著者	NRID 1000030779161 1000030779161 ORCID 0000-0001-7131-1074 0000-0001-7131-1074 researchmap permalink registered20221012 registered20221012 Scopus Author ID 58374671000 58374671000 著者名 Hara, Tomoaki
	NRID 1000050977204 1000050977204 ORCID 0000-0002-7220-5887 0000-0002-7220-5887 researchmap permalink sikunmeng sikunmeng Scopus Author ID 58484799900 58484799900 著者名 Meng, Sikun
	NRID 1000010636830 1000010636830 researchmap permalink daisukem daisukem Scopus Author ID 35315737900 35315737900 著者名 Motooka, Daisuke
	NRID 1000070962736 1000070962736 著者名 Arao, Yasuko
	著者名 Saito, Yoshiko
	著者名 Rennie, Sarah
	著者名 Uchida, Shizuka
	著者名 Ofusa, Ken
	著者名 Arai, Takahiro
	著者名 Konno, Masamitsu
	NRID 1000040368303 1000040368303 ORCID 0000-0002-4615-2638 0000-0002-4615-2638 researchmap permalink TarohSatoh TarohSatoh Scopus Author ID 12040860900 12040860900 著者名 Satoh, Taroh
	NRID 1000010280736 1000010280736 ORCID 0000-0002-0632-6517 0000-0002-0632-6517 researchmap permalink read0103186 read0103186 Scopus Author ID 7402978600 7402978600 著者名 Ishii, Hideshi
キーワード等	And niacin restriction (MTNR)
	Gut microbiota
	Intestinal transcriptome
	Methionine
	Methionine restriction (MR)
	Tryptophan
	Tryptophan and niacin restriction (TNR)
内容	Hara T., Meng S., Motooka D., et al. Deficiencies in methionine, tryptophan, and niacin remodels intestinal transcriptome and gut microbiota in female mice. Scientific Reports 15, 36155 (2025); https://doi.org/10.1038/s41598-025-18046-2.
抄録	Caloric restriction is well-established as a robust intervention that may extend lifespan and improve metabolic health across species with effects that are increasingly attributed to both host metabolic remodeling and alterations in the gut microbiota. Recent studies suggest that restricting specific dietary components can replicate these benefits. While methionine and branched-chain amino acid restriction improve metabolism and modulate the gut microbiome, the effects of other nutrients remain unclear. Here, we explore the effects of methionine, tryptophan and niacin deprivation on host intestinal gene expression and gut microbiota using female murine models. Through transcriptomic analysis of the intestinal tissue, we found that transient dietary restriction of methionine, tryptophan, and niacin induced significant changes in intestinal gene expression, particularly in genes involved in oxidative phosphorylation and ATP production. Single-cell analysis revealed that dietary restriction of those nutrients led to an increase in intestinal immune cell populations. Gut microbiota profiling also revealed that transient deprivation of those nutrients resulted in changes in microbial composition, with an increased relative abundance of Lactobacillus species observed in some cases. Our findings highlight the potential of targeted nutrient restriction as a strategy to reprogram host-microbiome interactions.
出版者	Nature Research
収録物名	Scientific Reports
巻(号)	15
ページ	36155
刊行年月	2025-10-16
言語	英語
ハンドルURL	https://hdl.handle.net/11094/103486
eISSN	20452322
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1038/s41598-025-18046-2
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Scientific Reports. 2025, 15, p. 36155
DC.title	Deficiencies in methionine, tryptophan, and niacin remodels intestinal transcriptome and gut microbiota in female mice
DC.creator	Hara, Tomoaki
	Meng, Sikun
	Motooka, Daisuke
	Arao, Yasuko
	Saito, Yoshiko
	Rennie, Sarah
	Uchida, Shizuka
	Ofusa, Ken
	Arai, Takahiro
	Konno, Masamitsu
	Satoh, Taroh
	Ishii, Hideshi
DC.publisher	Nature Research
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2025-10-16
DC.identifier	https://doi.org/10.1038/s41598-025-18046-2
DC.subject	And niacin restriction (MTNR)
	Gut microbiota
	Intestinal transcriptome
	Methionine
	Methionine restriction (MR)
	Tryptophan
	Tryptophan and niacin restriction (TNR)
DC.description	Hara T., Meng S., Motooka D., et al. Deficiencies in methionine, tryptophan, and niacin remodels intestinal transcriptome and gut microbiota in female mice. Scientific Reports 15, 36155 (2025); https://doi.org/10.1038/s41598-025-18046-2.
DCTERMS.abstract	Caloric restriction is well-established as a robust intervention that may extend lifespan and improve metabolic health across species with effects that are increasingly attributed to both host metabolic remodeling and alterations in the gut microbiota. Recent studies suggest that restricting specific dietary components can replicate these benefits. While methionine and branched-chain amino acid restriction improve metabolism and modulate the gut microbiome, the effects of other nutrients remain unclear. Here, we explore the effects of methionine, tryptophan and niacin deprivation on host intestinal gene expression and gut microbiota using female murine models. Through transcriptomic analysis of the intestinal tissue, we found that transient dietary restriction of methionine, tryptophan, and niacin induced significant changes in intestinal gene expression, particularly in genes involved in oxidative phosphorylation and ATP production. Single-cell analysis revealed that dietary restriction of those nutrients led to an increase in intestinal immune cell populations. Gut microbiota profiling also revealed that transient deprivation of those nutrients resulted in changes in microbial composition, with an increased relative abundance of Lactobacillus species observed in some cases. Our findings highlight the potential of targeted nutrient restriction as a strategy to reprogram host-microbiome interactions.
DC.rights	This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
DC.format	103486
citation_title	Deficiencies in methionine, tryptophan, and niacin remodels intestinal transcriptome and gut microbiota in female mice
citation_author	Hara, Tomoaki
	Meng, Sikun
	Motooka, Daisuke
	Arao, Yasuko
	Saito, Yoshiko
	Rennie, Sarah
	Uchida, Shizuka
	Ofusa, Ken
	Arai, Takahiro
	Konno, Masamitsu
	Satoh, Taroh
	Ishii, Hideshi
citation_publisher	Nature Research
citation_language	英語
citation_date	2025-10-16
citation_journal_title	Scientific Reports
citation_volume	15
citation_firstpage	36155
citation_doi	https://doi.org/10.1038/s41598-025-18046-2
citation_public_url	https://hdl.handle.net/11094/103486

Deficiencies in methionine, tryptophan, and niacin remodels intestinal transcriptome and gut microbiota in female mice

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