Silica nanoparticles reduce fetal weight in mice and induce an inflammatory response in human extravillous trophoblast cells

Yamamoto, Rena; Higashisaka, Kazuma; Sakai, Risa; Nakamoto, Yurina; Serizawa, Momoe; Haga, Yuya; Tsutsumi, Yasuo

doi:https://doi.org/10.1016/j.bbrc.2025.152803

Silica nanoparticles reduce fetal weight in mice and induce an inflammatory response in human extravillous trophoblast cells

Yamamoto, Rena; Higashisaka, Kazuma; Sakai, Risa et al.

Biochemical and Biophysical Research Communications, 2025, 788, 152803

アクセス数:13件（2026-01-13 16:30 集計）

固定URL: https://hdl.handle.net/11094/103512

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タイトル	Silica nanoparticles reduce fetal weight in mice and induce an inflammatory response in human extravillous trophoblast cells
著者	著者名 Yamamoto, Rena
	NRID 1000020646757 1000020646757 researchmap permalink read646757 read646757 Scopus Author ID 35603386200 35603386200 著者名 Higashisaka, Kazuma
	著者名 Sakai, Risa
	著者名 Nakamoto, Yurina
	著者名 Serizawa, Momoe
	NRID 1000030888863 1000030888863 ORCID 0000-0003-4148-2256 0000-0003-4148-2256 researchmap permalink 30888863 30888863 Scopus Author ID 57211023415 57211023415 著者名 Haga, Yuya
	NRID 1000050263306 1000050263306 researchmap permalink ytsutsumi ytsutsumi Scopus Author ID 7202474093 7202474093 著者名 Tsutsumi, Yasuo
キーワード等	Extravillous trophoblast cells
	Fetal toxicity
	Inflammation
	Placental toxicity
	Silica nanoparticle
内容	Yamamoto R., Higashisaka K., Sakai R., et al. Silica nanoparticles reduce fetal weight in mice and induce an inflammatory response in human extravillous trophoblast cells. Biochemical and Biophysical Research Communications 788, 152803 (2025); https://doi.org/10.1016/j.bbrc.2025.152803.
抄録	Advances in nanotechnology make the exposure to nanoparticles inevitable, potentially resulting in unexpected biological effects, particularly in pregnant women and fetuses. In this study, we used silica nanoparticles with a size of 10 nm (nSP10) and evaluated their toxicity during pregnancy in BALB/c mice. To evaluate the effect of nSP10 on the remodeling of uterine spiral arteries by extravillous trophoblast (EVT) cells, we used the human placental cell line HTR-8/SVneo, a model for EVT cells. On gestational day (GD) 10, mice were intravenously injected with a single dose of nSP10. On GD17, placental weight was not changed significantly, but fetal weight was significantly decreased by the treatment. In wound healing assay, nSP10 treatment significantly decreased the relative migration area of HTR-8/SVneo cells in a concentration-dependent manner, suggesting that nSP10 inhibits cell migration. In the cytokine array assay, nSP10-treated cells tended to produce more IL-6 and IL-8 than non-treated cells. In ELISA, nSP10 significantly increased IL-6 and IL-8 production, and JSH-23 (an inhibitor of NF-κB nuclear translocation) significantly suppressed this effect, suggesting that nSP10 increases IL-6 and IL-8 production by inducing nuclear translocation of NF-κB. Our data suggest that nSP10 reduces mouse fetal weight and may inhibit migration and induce inflammatory response of human extravillous trophoblast cells.
出版者	Elsevier B.V.
収録物名	Biochemical and Biophysical Research Communications
巻(号)	788
ページ	152803
刊行年月	2025-10-16
言語	英語
ハンドルURL	https://hdl.handle.net/11094/103512
PISSN	0006291X
eISSN	10902104
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1016/j.bbrc.2025.152803
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Biochemical and Biophysical Research Communications. 2025, 788, p. 152803
DC.title	Silica nanoparticles reduce fetal weight in mice and induce an inflammatory response in human extravillous trophoblast cells
DC.creator	Yamamoto, Rena
	Higashisaka, Kazuma
	Sakai, Risa
	Nakamoto, Yurina
	Serizawa, Momoe
	Haga, Yuya
	Tsutsumi, Yasuo
DC.publisher	Elsevier B.V.
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2025-10-16
DC.identifier	https://doi.org/10.1016/j.bbrc.2025.152803
DC.subject	Extravillous trophoblast cells
	Fetal toxicity
	Inflammation
	Placental toxicity
	Silica nanoparticle
DC.description	Yamamoto R., Higashisaka K., Sakai R., et al. Silica nanoparticles reduce fetal weight in mice and induce an inflammatory response in human extravillous trophoblast cells. Biochemical and Biophysical Research Communications 788, 152803 (2025); https://doi.org/10.1016/j.bbrc.2025.152803.
DCTERMS.abstract	Advances in nanotechnology make the exposure to nanoparticles inevitable, potentially resulting in unexpected biological effects, particularly in pregnant women and fetuses. In this study, we used silica nanoparticles with a size of 10 nm (nSP10) and evaluated their toxicity during pregnancy in BALB/c mice. To evaluate the effect of nSP10 on the remodeling of uterine spiral arteries by extravillous trophoblast (EVT) cells, we used the human placental cell line HTR-8/SVneo, a model for EVT cells. On gestational day (GD) 10, mice were intravenously injected with a single dose of nSP10. On GD17, placental weight was not changed significantly, but fetal weight was significantly decreased by the treatment. In wound healing assay, nSP10 treatment significantly decreased the relative migration area of HTR-8/SVneo cells in a concentration-dependent manner, suggesting that nSP10 inhibits cell migration. In the cytokine array assay, nSP10-treated cells tended to produce more IL-6 and IL-8 than non-treated cells. In ELISA, nSP10 significantly increased IL-6 and IL-8 production, and JSH-23 (an inhibitor of NF-κB nuclear translocation) significantly suppressed this effect, suggesting that nSP10 increases IL-6 and IL-8 production by inducing nuclear translocation of NF-κB. Our data suggest that nSP10 reduces mouse fetal weight and may inhibit migration and induce inflammatory response of human extravillous trophoblast cells.
DC.rights	This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
DC.format	103512
citation_title	Silica nanoparticles reduce fetal weight in mice and induce an inflammatory response in human extravillous trophoblast cells
citation_author	Yamamoto, Rena
	Higashisaka, Kazuma
	Sakai, Risa
	Nakamoto, Yurina
	Serizawa, Momoe
	Haga, Yuya
	Tsutsumi, Yasuo
citation_publisher	Elsevier B.V.
citation_language	英語
citation_date	2025-10-16
citation_journal_title	Biochemical and Biophysical Research Communications
citation_volume	788
citation_firstpage	152803
citation_doi	https://doi.org/10.1016/j.bbrc.2025.152803
citation_public_url	https://hdl.handle.net/11094/103512

Silica nanoparticles reduce fetal weight in mice and induce an inflammatory response in human extravillous trophoblast cells

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