Expression analysis of genes including Zfhx4 in mice and zebrafish reveals a temporospatial conserved molecular basis underlying craniofacial development

Liu, Shujie; Xu, Lin; Kashima, Makoto; Narumi, Rika; Takahata, Yoshifumi; Nakamura, Eriko; Shibuya, Hirotoshi; Tamura, Masaru; Shida, Yuki; Inubushi, Toshihiro; Nukada, Yuko; Miyazawa, Masaaki; Hata, Kenji; Nishimura, Riko; Yamashiro, Takashi; Tasaki, Junichi; Kurosaka, Hiroshi

doi:https://doi.org/10.1002/dvdy.740

Expression analysis of genes including Zfhx4 in mice and zebrafish reveals a temporospatial conserved molecular basis underlying craniofacial development

Liu, Shujie; Xu, Lin; Kashima, Makoto et al.

Developmental Dynamics, 2024, 254(3), 257-271

アクセス数:172件（2026-02-15 21:10 集計）

固定URL: https://hdl.handle.net/11094/103547

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タイトル	Expression analysis of genes including Zfhx4 in mice and zebrafish reveals a temporospatial conserved molecular basis underlying craniofacial development
著者	著者名 Liu, Shujie
	著者名 Xu, Lin
	NRID 1000010780562 1000010780562 著者名 Kashima, Makoto
	著者名 Narumi, Rika
	NRID 1000060635845 1000060635845 ORCID 0000-0002-3322-0515 0000-0002-3322-0515 researchmap permalink yoshifumitakahata yoshifumitakahata Scopus Author ID 22837135800 22837135800 著者名 Takahata, Yoshifumi
	NRID 1000000755069 1000000755069 researchmap permalink nakamuraeriko nakamuraeriko Scopus Author ID 56025920800 56025920800 著者名 Nakamura, Eriko
	NRID 1000010828346 1000010828346 著者名 Shibuya, Hirotoshi
	NRID 1000050370119 1000050370119 著者名 Tamura, Masaru
	著者名 Shida, Yuki
	NRID 1000030550941 1000030550941 ORCID 0000-0001-5303-4763 0000-0001-5303-4763 researchmap permalink opathone0141 opathone0141 Scopus Author ID 23097933700 23097933700 著者名 Inubushi, Toshihiro
	著者名 Nukada, Yuko
	著者名 Miyazawa, Masaaki
	NRID 1000080444496 1000080444496 ORCID 0000-0003-4367-9343 0000-0003-4367-9343 researchmap permalink read0107167 read0107167 Scopus Author ID 8952419300 8952419300 著者名 Hata, Kenji
	NRID 1000060294112 1000060294112 著者名 Nishimura, Riko
	NRID 1000070294428 1000070294428 ORCID 0000-0002-4419-9643 0000-0002-4419-9643 researchmap permalink read0051793 read0051793 Scopus Author ID 55416765600 55416765600 著者名 Yamashiro, Takashi
	著者名 Tasaki, Junichi
	NRID 1000020509369 1000020509369 ORCID 0000-0002-8956-5467 0000-0002-8956-5467 researchmap permalink kurosakahiroshi kurosakahiroshi Scopus Author ID 11839162500 11839162500 著者名 Kurosaka, Hiroshi
キーワード等	cranial neural crest cells
	disease models
	loss-of-function analysis
	palatogenesis
	stage-matched gene expression analysis
	temporal expression patterns
内容	Liu S., Xu L., Kashima M., et al. Expression analysis of genes including Zfhx4 in mice and zebrafish reveals a temporospatial conserved molecular basis underlying craniofacial development. Developmental Dynamics 254, 257 (2025); https://doi.org/10.1002/dvdy.740.
抄録	Background: Embryonic craniofacial development involves several cellular and molecular events that are evolutionarily conserved among vertebrates. Vertebrate models such as mice and zebrafish have been used to investigate the molecular and cellular etiologies underlying human craniofacial disorders, including orofacial clefts. However, the molecular mechanisms underlying embryonic development in these two species are unknown. Therefore, elucidating the shared mechanisms of craniofacial development between disease models is crucial to understanding the underlying mechanisms of phenotypes in individual species. Results: We selected mice and zebrafish as model organisms to compare various events during embryonic craniofacial development. We identified genes (Sox9, Zfhx3 and 4, Cjun, and Six1) exhibiting similar temporal expression patterns between these species through comprehensive and stage-matched gene expression analyses. Expression analysis revealed similar gene expression in hypothetically corresponding tissues, such as the mice palate and zebrafish ethmoid plate. Furthermore, loss-of-function analysis of Zfhx4/zfhx4, a causative gene of human craniofacial anomalies including orofacial cleft, in both species resulted in deformed skeletal elements such as the palatine and ethmoid plate in mice and zebrafish, respectively. Conclusions: These results demonstrate that these disease models share common molecular mechanisms, highlighting their usefulness in modeling craniofacial defects in humans.
出版者	John Wiley and Sons Inc
収録物名	Developmental Dynamics
巻(号)	254(3)
ページ	257-271
刊行年月	2024-09-25
言語	英語
ハンドルURL	https://hdl.handle.net/11094/103547
PISSN	10588388
eISSN	10970177
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1002/dvdy.740
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Developmental Dynamics. 2024, 254(3), p. 257-271
DC.title	Expression analysis of genes including Zfhx4 in mice and zebrafish reveals a temporospatial conserved molecular basis underlying craniofacial development
DC.creator	Liu, Shujie
	Xu, Lin
	Kashima, Makoto
	Narumi, Rika
	Takahata, Yoshifumi
	Nakamura, Eriko
	Shibuya, Hirotoshi
	Tamura, Masaru
	Shida, Yuki
	Inubushi, Toshihiro
	Nukada, Yuko
	Miyazawa, Masaaki
	Hata, Kenji
	Nishimura, Riko
	Yamashiro, Takashi
	Tasaki, Junichi
	Kurosaka, Hiroshi
DC.publisher	John Wiley and Sons Inc
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2024-09-25
DC.identifier	https://doi.org/10.1002/dvdy.740
DC.subject	cranial neural crest cells
	disease models
	loss-of-function analysis
	palatogenesis
	stage-matched gene expression analysis
	temporal expression patterns
DC.description	Liu S., Xu L., Kashima M., et al. Expression analysis of genes including Zfhx4 in mice and zebrafish reveals a temporospatial conserved molecular basis underlying craniofacial development. Developmental Dynamics 254, 257 (2025); https://doi.org/10.1002/dvdy.740.
DCTERMS.abstract	Background: Embryonic craniofacial development involves several cellular and molecular events that are evolutionarily conserved among vertebrates. Vertebrate models such as mice and zebrafish have been used to investigate the molecular and cellular etiologies underlying human craniofacial disorders, including orofacial clefts. However, the molecular mechanisms underlying embryonic development in these two species are unknown. Therefore, elucidating the shared mechanisms of craniofacial development between disease models is crucial to understanding the underlying mechanisms of phenotypes in individual species. Results: We selected mice and zebrafish as model organisms to compare various events during embryonic craniofacial development. We identified genes (Sox9, Zfhx3 and 4, Cjun, and Six1) exhibiting similar temporal expression patterns between these species through comprehensive and stage-matched gene expression analyses. Expression analysis revealed similar gene expression in hypothetically corresponding tissues, such as the mice palate and zebrafish ethmoid plate. Furthermore, loss-of-function analysis of Zfhx4/zfhx4, a causative gene of human craniofacial anomalies including orofacial cleft, in both species resulted in deformed skeletal elements such as the palatine and ethmoid plate in mice and zebrafish, respectively. Conclusions: These results demonstrate that these disease models share common molecular mechanisms, highlighting their usefulness in modeling craniofacial defects in humans.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	103547
citation_title	Expression analysis of genes including Zfhx4 in mice and zebrafish reveals a temporospatial conserved molecular basis underlying craniofacial development
citation_author	Liu, Shujie
	Xu, Lin
	Kashima, Makoto
	Narumi, Rika
	Takahata, Yoshifumi
	Nakamura, Eriko
	Shibuya, Hirotoshi
	Tamura, Masaru
	Shida, Yuki
	Inubushi, Toshihiro
	Nukada, Yuko
	Miyazawa, Masaaki
	Hata, Kenji
	Nishimura, Riko
	Yamashiro, Takashi
	Tasaki, Junichi
	Kurosaka, Hiroshi
citation_publisher	John Wiley and Sons Inc
citation_language	英語
citation_date	2024-09-25
citation_journal_title	Developmental Dynamics
citation_volume	254
citation_issue	3
citation_firstpage	257
citation_lastpage	271
citation_doi	https://doi.org/10.1002/dvdy.740
citation_public_url	https://hdl.handle.net/11094/103547

Expression analysis of genes including Zfhx4 in mice and zebrafish reveals a temporospatial conserved molecular basis underlying craniofacial development

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