Branchiomeric Muscle Development Requires Proper Retinoic Acid Signaling

Wang, Qi; Xu, Lin; Miura, Jiro; Saha, Mithun Kumar; Uemura, Yume; Sandell, Lisa L.; Trainor, Paul A.; Yamashiro, Takashi; Kurosaka, Hiroshi

doi:https://doi.org/10.3389/fcell.2021.596838

Branchiomeric Muscle Development Requires Proper Retinoic Acid Signaling

Wang, Qi; Xu, Lin; Miura, Jiro et al.

Frontiers in Cell and Developmental Biology, 2021, 9, 596838

アクセス数:106件（2026-02-15 21:36 集計）

固定URL: https://hdl.handle.net/11094/103551

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タイトル	Branchiomeric Muscle Development Requires Proper Retinoic Acid Signaling
著者	著者名 Wang, Qi
	著者名 Xu, Lin
	NRID 1000070437383 1000070437383 ORCID 0000-0002-0000-025X 0000-0002-0000-025X researchmap permalink read0091676 read0091676 Scopus Author ID 13205291100 13205291100 著者名 Miura, Jiro
	著者名 Saha, Mithun Kumar
	NRID 1000090848251 1000090848251 著者名 Uemura, Yume
	著者名 Sandell, Lisa L.
	著者名 Trainor, Paul A.
	NRID 1000070294428 1000070294428 ORCID 0000-0002-4419-9643 0000-0002-4419-9643 researchmap permalink read0051793 read0051793 Scopus Author ID 55416765600 55416765600 著者名 Yamashiro, Takashi
	NRID 1000020509369 1000020509369 ORCID 0000-0002-8956-5467 0000-0002-8956-5467 researchmap permalink kurosakahiroshi kurosakahiroshi Scopus Author ID 11839162500 11839162500 著者名 Kurosaka, Hiroshi
キーワード等	cranial muscle development
	craniofacial abnormalities
	muscle differentiation
	muscle progenitor cell
	retinoic acid signaling
内容	Wang Q., Xu L., Miura J., et al. Branchiomeric Muscle Development Requires Proper Retinoic Acid Signaling. Frontiers in Cell and Developmental Biology 9, 596838 (2021); https://doi.org/10.3389/fcell.2021.596838.
抄録	The first and second branchiomeric (branchial arch) muscles are craniofacial muscles that derive from branchial arch mesoderm. In mammals, this set of muscles is indispensable for jaw movement and facial expression. Defects during embryonic development that result in congenital partial absence of these muscles can have significant impact on patients’ quality of life. However, the detailed molecular and cellular mechanisms that regulate branchiomeric muscle development remains poorly understood. Herein we investigated the role of retinoic acid (RA) signaling in developing branchiomeric muscles using mice as a model. We administered all-trans RA (25 mg/kg body weight) to Institute of Cancer Research (ICR) pregnant mice by gastric intubation from E8.5 to E10.5. In their embryos at E13.5, we found that muscles derived from the first branchial arch (temporalis, masseter) and second branchial arch (frontalis, orbicularis oculi) were severely affected or undetectable, while other craniofacial muscles were hypoplastic. We detected elevated cell death in the branchial arch mesoderm cells in RA-treated embryos, suggesting that excessive RA signaling reduces the survival of precursor cells of branchiomeric muscles, resulting in the development of hypoplastic craniofacial muscles. In order to uncover the signaling pathway(s) underlying this etiology, we focused on Pitx2, Tbx1, and MyoD1, which are critical for cranial muscle development. Noticeably reduced expression of all these genes was detected in the first and second branchial arch of RA-treated embryos. Moreover, elevated RA signaling resulted in a reduction in Dlx5 and Dlx6 expression in cranial neural crest cells (CNCCs), which disturbed their interactions with branchiomeric mesoderm cells. Altogether, we discovered that embryonic craniofacial muscle defects caused by excessive RA signaling were associated with the downregulation of Pitx2, Tbx1, MyoD1, and Dlx5/6, and reduced survival of cranial myogenic precursor cells.
出版者	Frontiers Media S.A.
収録物名	Frontiers in Cell and Developmental Biology
巻(号)	9
ページ	596838
刊行年月	2021-07-09
言語	英語
ハンドルURL	https://hdl.handle.net/11094/103551
eISSN	2296634X
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.3389/fcell.2021.596838
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Frontiers in Cell and Developmental Biology. 2021, 9, p. 596838
DC.title	Branchiomeric Muscle Development Requires Proper Retinoic Acid Signaling
DC.creator	Wang, Qi
	Xu, Lin
	Miura, Jiro
	Saha, Mithun Kumar
	Uemura, Yume
	Sandell, Lisa L.
	Trainor, Paul A.
	Yamashiro, Takashi
	Kurosaka, Hiroshi
DC.publisher	Frontiers Media S.A.
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2021-07-09
DC.identifier	https://doi.org/10.3389/fcell.2021.596838
DC.subject	cranial muscle development
	craniofacial abnormalities
	muscle differentiation
	muscle progenitor cell
	retinoic acid signaling
DC.description	Wang Q., Xu L., Miura J., et al. Branchiomeric Muscle Development Requires Proper Retinoic Acid Signaling. Frontiers in Cell and Developmental Biology 9, 596838 (2021); https://doi.org/10.3389/fcell.2021.596838.
DCTERMS.abstract	The first and second branchiomeric (branchial arch) muscles are craniofacial muscles that derive from branchial arch mesoderm. In mammals, this set of muscles is indispensable for jaw movement and facial expression. Defects during embryonic development that result in congenital partial absence of these muscles can have significant impact on patients’ quality of life. However, the detailed molecular and cellular mechanisms that regulate branchiomeric muscle development remains poorly understood. Herein we investigated the role of retinoic acid (RA) signaling in developing branchiomeric muscles using mice as a model. We administered all-trans RA (25 mg/kg body weight) to Institute of Cancer Research (ICR) pregnant mice by gastric intubation from E8.5 to E10.5. In their embryos at E13.5, we found that muscles derived from the first branchial arch (temporalis, masseter) and second branchial arch (frontalis, orbicularis oculi) were severely affected or undetectable, while other craniofacial muscles were hypoplastic. We detected elevated cell death in the branchial arch mesoderm cells in RA-treated embryos, suggesting that excessive RA signaling reduces the survival of precursor cells of branchiomeric muscles, resulting in the development of hypoplastic craniofacial muscles. In order to uncover the signaling pathway(s) underlying this etiology, we focused on Pitx2, Tbx1, and MyoD1, which are critical for cranial muscle development. Noticeably reduced expression of all these genes was detected in the first and second branchial arch of RA-treated embryos. Moreover, elevated RA signaling resulted in a reduction in Dlx5 and Dlx6 expression in cranial neural crest cells (CNCCs), which disturbed their interactions with branchiomeric mesoderm cells. Altogether, we discovered that embryonic craniofacial muscle defects caused by excessive RA signaling were associated with the downregulation of Pitx2, Tbx1, MyoD1, and Dlx5/6, and reduced survival of cranial myogenic precursor cells.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	103551
citation_title	Branchiomeric Muscle Development Requires Proper Retinoic Acid Signaling
citation_author	Wang, Qi
	Xu, Lin
	Miura, Jiro
	Saha, Mithun Kumar
	Uemura, Yume
	Sandell, Lisa L.
	Trainor, Paul A.
	Yamashiro, Takashi
	Kurosaka, Hiroshi
citation_publisher	Frontiers Media S.A.
citation_language	英語
citation_date	2021-07-09
citation_journal_title	Frontiers in Cell and Developmental Biology
citation_volume	9
citation_firstpage	596838
citation_doi	https://doi.org/10.3389/fcell.2021.596838
citation_public_url	https://hdl.handle.net/11094/103551

Branchiomeric Muscle Development Requires Proper Retinoic Acid Signaling

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