学術雑誌論文

学術雑誌論文

text

Algal Research. 2026, 93, p. 104442

Synergistic enhancement of biomass and lipid productivity in Chlorella sorokiniana co-cultured with engineered vitamin-producing Escherichia coli

Elsevier B.V.

英語

text

2026-01-01

https://doi.org/10.1016/j.algal.2025.104442

Tong C.Y., Tomita H., Miyazaki K., et al. Synergistic enhancement of biomass and lipid productivity in Chlorella sorokiniana co-cultured with engineered vitamin-producing Escherichia coli. Algal Research 93, 104442 (2026); https://doi.org/10.1016/j.algal.2025.104442.

Recognizing that approximately 50–70 % of green microalgae are vitamin auxotrophs, co-cultivation with vitamin-producing bacteria presents a promising strategy in boosting the microalgal growth, yet natural partnerships often lack efficiency. To address this research gap, this study establishes a synthetic vitamin-based mutualism by metabolically engineered Escherichia coli BL21(DE3) to enhanced vitamin production. Specifically, riboflavin biosynthetic zwf-fbp genes, cobalamin biosynthetic cobU-cobC genes, and biotin biosynthetic bioF-bioD genes were cloned in three compatible T7 promoter-based expression plasmids and were co-introduced into the bacterial host strain, yielding a recombinant strain RS04. Chlorella sorokiniana was then co-cultured with RS04 and examined for cell growth and lipid accumulation. Results showed at least a four-fold increase in algal growth as indicated by Chl-a fluorescence intensity (a resulting microalgal-to-bacterial dry weight ratio of 6.2), at Day 7, compared to the controls. At the early co-cultivation phase, microalgae appeared to sustain bacterial viability, exhibiting a bacterial growth rescue effect from Day 14–28. Based on the observed vitamin levels in the cultivation medium and preliminary interpretation, a potential sequential vitamin assimilation model was proposed: biotin (initial uptake)➔ cobalamin (consistent need)➔ riboflavin (slowest). In terms of biodiesel properties, this consortium enhanced cold weather performance (~18 °C cloud point; 4.8 mm2 s−1 kinematic viscosity) and fuel efficiency (~40 MJ kg−1). This study demonstrated that microalgae produce lipids in a short time under a highly stable co-cultivation system.

This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

103573

Synergistic enhancement of biomass and lipid productivity in Chlorella sorokiniana co-cultured with engineered vitamin-producing Escherichia coli

Elsevier B.V.

英語

2026-01-01

Algal Research

93

104442

https://doi.org/10.1016/j.algal.2025.104442

https://hdl.handle.net/11094/103573