Effects of electrolyte composition and applied voltage on methane generation and microbial community shifts in the electromethanogenesis system

Direstiyani, Lucky Caesar; Inoue, Daisuke; Ike, Michihiko

doi:https://doi.org/10.1016/j.bej.2025.110035

Effects of electrolyte composition and applied voltage on methane generation and microbial community shifts in the electromethanogenesis system

Direstiyani, Lucky Caesar; Inoue, Daisuke; Ike, Michihiko

Biochemical Engineering Journal, 2026, 227, 110035

アクセス数:53件（2026-02-15 21:10 集計）

固定URL: https://hdl.handle.net/11094/103575

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タイトル	Effects of electrolyte composition and applied voltage on methane generation and microbial community shifts in the electromethanogenesis system
著者	著者名 Direstiyani, Lucky Caesar
	NRID 1000070448091 1000070448091 ORCID 0000-0002-9216-8198 0000-0002-9216-8198 researchmap permalink DaisukeINOUE DaisukeINOUE Scopus Author ID 55723196300 55723196300 著者名 Inoue, Daisuke
	NRID 1000040222856 1000040222856 ORCID 0000-0001-6237-8103 0000-0001-6237-8103 researchmap permalink ikemichi ikemichi Scopus Author ID 55551254800 55551254800 著者名 Ike, Michihiko
キーワード等	Applied voltage
	Electrode biofilm
	Electrolyte
	Electromethanogenesis
	Microbial community
内容	Direstiyani L.C., Inoue D., Ike M.. Effects of electrolyte composition and applied voltage on methane generation and microbial community shifts in the electromethanogenesis system. Biochemical Engineering Journal 227, 110035 (2026); https://doi.org/10.1016/j.bej.2025.110035.
抄録	An electromethanogenesis (EM) system was successfully established using non-acclimated anaerobic digestion sludge as the inoculum. This study aimed to evaluate the EM performance by varying the electrolyte composition and the applied voltage. Alterations in the microbial community associated with CH4 generation and bioelectrochemchemical performance were also investigated. The findings indicated that the use of organic-rich electrolyte with a low applied voltage of 0.15 V showed a positive correlation with enhanced CH4 generation up to 59 % and a CH4 yield of 223.13 mmol day−1 m−2 which was ten times higher than the operation using the same electrolyte with an applied voltage of 0.35 V. Microbial community analysis revealed a shift of dominant methanogens from Methanosaeta to Methanosarcina and Methanoculleus at the cathodic biofilms when operated with organic-rich electrolyte at low voltage of 0.15 V. The presence of electroactive bacteria, such as DMER64 and JGI-0000079-D21 , and syntrophic bacteria, including Desulfovibrio and Petrimonas , suggested the development of syntrophic interactions that strengthen biofilm resilience and the overall performance of the EM system. The microbial interaction network also emphasized the significance of electrolyte composition and adequate applied voltage in shaping microbial biofilms for efficient CH4 generation. The findings of this study accentuate the roles of sufficient electrolyte composition and low-voltage in enhancing the EM performance and corroborate the synergistic advantages of the EM system.
出版者	Elsevier B.V.
収録物名	Biochemical Engineering Journal
巻(号)	227
ページ	110035
刊行年月	2026-03-01
言語	英語
ハンドルURL	https://hdl.handle.net/11094/103575
PISSN	1369703X
eISSN	1873295X
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1016/j.bej.2025.110035
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Biochemical Engineering Journal. 2026, 227, p. 110035
DC.title	Effects of electrolyte composition and applied voltage on methane generation and microbial community shifts in the electromethanogenesis system
DC.creator	Direstiyani, Lucky Caesar
	Inoue, Daisuke
	Ike, Michihiko
DC.publisher	Elsevier B.V.
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2026-03-01
DC.identifier	https://doi.org/10.1016/j.bej.2025.110035
DC.subject	Applied voltage
	Electrode biofilm
	Electrolyte
	Electromethanogenesis
	Microbial community
DC.description	Direstiyani L.C., Inoue D., Ike M.. Effects of electrolyte composition and applied voltage on methane generation and microbial community shifts in the electromethanogenesis system. Biochemical Engineering Journal 227, 110035 (2026); https://doi.org/10.1016/j.bej.2025.110035.
DCTERMS.abstract	An electromethanogenesis (EM) system was successfully established using non-acclimated anaerobic digestion sludge as the inoculum. This study aimed to evaluate the EM performance by varying the electrolyte composition and the applied voltage. Alterations in the microbial community associated with CH4 generation and bioelectrochemchemical performance were also investigated. The findings indicated that the use of organic-rich electrolyte with a low applied voltage of 0.15 V showed a positive correlation with enhanced CH4 generation up to 59 % and a CH4 yield of 223.13 mmol day−1 m−2 which was ten times higher than the operation using the same electrolyte with an applied voltage of 0.35 V. Microbial community analysis revealed a shift of dominant methanogens from Methanosaeta to Methanosarcina and Methanoculleus at the cathodic biofilms when operated with organic-rich electrolyte at low voltage of 0.15 V. The presence of electroactive bacteria, such as DMER64 and JGI-0000079-D21 , and syntrophic bacteria, including Desulfovibrio and Petrimonas , suggested the development of syntrophic interactions that strengthen biofilm resilience and the overall performance of the EM system. The microbial interaction network also emphasized the significance of electrolyte composition and adequate applied voltage in shaping microbial biofilms for efficient CH4 generation. The findings of this study accentuate the roles of sufficient electrolyte composition and low-voltage in enhancing the EM performance and corroborate the synergistic advantages of the EM system.
DC.rights	This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
DC.format	103575
citation_title	Effects of electrolyte composition and applied voltage on methane generation and microbial community shifts in the electromethanogenesis system
citation_author	Direstiyani, Lucky Caesar
	Inoue, Daisuke
	Ike, Michihiko
citation_publisher	Elsevier B.V.
citation_language	英語
citation_date	2026-03-01
citation_journal_title	Biochemical Engineering Journal
citation_volume	227
citation_firstpage	110035
citation_doi	https://doi.org/10.1016/j.bej.2025.110035
citation_public_url	https://hdl.handle.net/11094/103575

Effects of electrolyte composition and applied voltage on methane generation and microbial community shifts in the electromethanogenesis system

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