Occupational Exposure to Factory-Derived Particulate Matter 2.5 Induces Cutaneous Toxicity in Workers and HaCaT Keratinocytes Through Oxidative Stress and Inflammation

Tanaka, Miku; Takaishi, Masayuki; Okuda, Iroha; Fujimoto, Toshiki; Tsuji, Mayumi; Okano, Yuri; Ishihara, Yasuhiro; Kono, Maori

doi:https://doi.org/10.1002/jat.70018

Occupational Exposure to Factory-Derived Particulate Matter 2.5 Induces Cutaneous Toxicity in Workers and HaCaT Keratinocytes Through Oxidative Stress and Inflammation

Tanaka, Miku; Takaishi, Masayuki; Okuda, Iroha et al.

Journal of Applied Toxicology, 2025

アクセス数:26件（2026-02-15 18:00 集計）

固定URL: https://hdl.handle.net/11094/103584

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タイトル	Occupational Exposure to Factory-Derived Particulate Matter 2.5 Induces Cutaneous Toxicity in Workers and HaCaT Keratinocytes Through Oxidative Stress and Inflammation
著者	著者名 Tanaka, Miku
	NRID 1000020898225 1000020898225 著者名 Takaishi, Masayuki
	著者名 Okuda, Iroha
	著者名 Fujimoto, Toshiki
	著者名 Tsuji, Mayumi
	著者名 Okano, Yuri
	NRID 1000080435073 1000080435073 著者名 Ishihara, Yasuhiro
	NRID 1000060880995 1000060880995 ORCID 0009-0004-9821-9656 0009-0004-9821-9656 researchmap permalink _maori _maori 著者名 Kono, Maori
キーワード等	cutaneous toxicity
	inflammation
	keratinocytes
	occupational exposure
	oxidative stress
	PM2.5
	skin barrier dysfunction
	welding fumes
内容	Tanaka M., Takaishi M., Okuda I., et al. Occupational Exposure to Factory-Derived Particulate Matter 2.5 Induces Cutaneous Toxicity in Workers and HaCaT Keratinocytes Through Oxidative Stress and Inflammation. Journal of Applied Toxicology , (2025); https://doi.org/10.1002/jat.70018.
抄録	This study aimed to evaluate the toxicological effects of occupational exposure to factory-derived particulate matter (PM2.5) on human skin, emphasizing its role as a potential target organ of environmental toxicants. An epidemiological survey comparing factory and nonfactory workers was conducted by assessing their skin elasticity and color on the neck and forehead. Carbonylated proteins and inflammatory cytokines were quantified using tape stripping, and stratum corneum (SC) desquamation and cell area were analyzed using Brilliant Green staining. In addition, HaCaT keratinocytes were exposed to factory-derived PM2.5 (welding fumes), and oxidative stress, inflammation, and barrier function were assessed using gene expression analysis and reporter assays. Factory workers showed reduced dermal elasticity, increased skin redness, elevated levels of carbonylated proteins and inflammatory cytokines (IFN-γ and CXCL10), and enhanced SC desquamation with smaller cell areas. Consistently, in vitro exposure of HaCaT keratinocytes to PM2.5 induced oxidative stress (activation of the antioxidant response element pathway), inflammatory responses (nuclear factor kappa B activation), and suggested barrier impairment. These findings reveal that chronic occupational exposure to welding fumes impairs skin structure and function through oxidative stress, inflammation, and barrier disruption, highlighting the skin as a relevant target organ in occupational toxicology.
出版者	John Wiley and Sons Ltd
収録物名	Journal of Applied Toxicology
刊行年月	2025-12-05
言語	英語
ハンドルURL	https://hdl.handle.net/11094/103584
PISSN	0260437X
eISSN	10991263
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1002/jat.70018
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Journal of Applied Toxicology. 2025
DC.title	Occupational Exposure to Factory-Derived Particulate Matter 2.5 Induces Cutaneous Toxicity in Workers and HaCaT Keratinocytes Through Oxidative Stress and Inflammation
DC.creator	Tanaka, Miku
	Takaishi, Masayuki
	Okuda, Iroha
	Fujimoto, Toshiki
	Tsuji, Mayumi
	Okano, Yuri
	Ishihara, Yasuhiro
	Kono, Maori
DC.publisher	John Wiley and Sons Ltd
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2025-12-05
DC.identifier	https://doi.org/10.1002/jat.70018
DC.subject	cutaneous toxicity
	inflammation
	keratinocytes
	occupational exposure
	oxidative stress
	PM2.5
	skin barrier dysfunction
	welding fumes
DC.description	Tanaka M., Takaishi M., Okuda I., et al. Occupational Exposure to Factory-Derived Particulate Matter 2.5 Induces Cutaneous Toxicity in Workers and HaCaT Keratinocytes Through Oxidative Stress and Inflammation. Journal of Applied Toxicology , (2025); https://doi.org/10.1002/jat.70018.
DCTERMS.abstract	This study aimed to evaluate the toxicological effects of occupational exposure to factory-derived particulate matter (PM2.5) on human skin, emphasizing its role as a potential target organ of environmental toxicants. An epidemiological survey comparing factory and nonfactory workers was conducted by assessing their skin elasticity and color on the neck and forehead. Carbonylated proteins and inflammatory cytokines were quantified using tape stripping, and stratum corneum (SC) desquamation and cell area were analyzed using Brilliant Green staining. In addition, HaCaT keratinocytes were exposed to factory-derived PM2.5 (welding fumes), and oxidative stress, inflammation, and barrier function were assessed using gene expression analysis and reporter assays. Factory workers showed reduced dermal elasticity, increased skin redness, elevated levels of carbonylated proteins and inflammatory cytokines (IFN-γ and CXCL10), and enhanced SC desquamation with smaller cell areas. Consistently, in vitro exposure of HaCaT keratinocytes to PM2.5 induced oxidative stress (activation of the antioxidant response element pathway), inflammatory responses (nuclear factor kappa B activation), and suggested barrier impairment. These findings reveal that chronic occupational exposure to welding fumes impairs skin structure and function through oxidative stress, inflammation, and barrier disruption, highlighting the skin as a relevant target organ in occupational toxicology.
DC.rights	This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
DC.format	103584
citation_title	Occupational Exposure to Factory-Derived Particulate Matter 2.5 Induces Cutaneous Toxicity in Workers and HaCaT Keratinocytes Through Oxidative Stress and Inflammation
citation_author	Tanaka, Miku
	Takaishi, Masayuki
	Okuda, Iroha
	Fujimoto, Toshiki
	Tsuji, Mayumi
	Okano, Yuri
	Ishihara, Yasuhiro
	Kono, Maori
citation_publisher	John Wiley and Sons Ltd
citation_language	英語
citation_date	2025-12-05
citation_journal_title	Journal of Applied Toxicology
citation_doi	https://doi.org/10.1002/jat.70018
citation_public_url	https://hdl.handle.net/11094/103584

Occupational Exposure to Factory-Derived Particulate Matter 2.5 Induces Cutaneous Toxicity in Workers and HaCaT Keratinocytes Through Oxidative Stress and Inflammation

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