Application of on-demand aqueous chlorine dioxide solution for non-surgical root canal treatment

Shimaoka, Tsuyoshi; Maezono, Hazuki; Ono, Shunka; Asahi, Yoko; Kawanishi, Yuzo; Klanliang, Kittipit; Takahashi, Yusuke; Hayashi, Mikako

doi:https://doi.org/10.1038/s41598-025-20131-5

Application of on-demand aqueous chlorine dioxide solution for non-surgical root canal treatment

Shimaoka, Tsuyoshi; Maezono, Hazuki; Ono, Shunka et al.

Scientific Reports, 2025, 15, 36215

アクセス数:51件（2026-02-15 20:42 集計）

固定URL: https://hdl.handle.net/11094/103668

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タイトル	Application of on-demand aqueous chlorine dioxide solution for non-surgical root canal treatment
著者	NRID 1000091001105 1000091001105 researchmap permalink t.shimaoka t.shimaoka 著者名 Shimaoka, Tsuyoshi
	NRID 1000000613390 1000000613390 ORCID 0000-0002-1929-2045 0000-0002-1929-2045 researchmap permalink h-maezono h-maezono Scopus Author ID 52463972400 52463972400 著者名 Maezono, Hazuki
	著者名 Ono, Shunka
	NRID 1000050456943 1000050456943 ORCID 0000-0001-9443-5790 0000-0001-9443-5790 researchmap permalink yasahi yasahi Scopus Author ID 7006117262 7006117262 著者名 Asahi, Yoko
	著者名 Kawanishi, Yuzo
	著者名 Klanliang, Kittipit
	NRID 1000060397693 1000060397693 ORCID 0000-0002-7974-1252 0000-0002-7974-1252 researchmap permalink read0210127 read0210127 Scopus Author ID 7408219812 7408219812 著者名 Takahashi, Yusuke
	NRID 1000040271027 1000040271027 researchmap permalink read0185244 read0185244 Scopus Author ID 26021225700 26021225700 著者名 Hayashi, Mikako
キーワード等	Biofilm
	Chlorine dioxide
	Cytotoxicity
	MA-T
	Osteoblast
	Periodontal ligament fibroblast
	Root canal irrigation
内容	Shimaoka T., Maezono H., Ono S., et al. Application of on-demand aqueous chlorine dioxide solution for non-surgical root canal treatment. Scientific Reports 15, 36215 (2025); https://doi.org/10.1038/s41598-025-20131-5.
抄録	Oral biofilms contribute to apical periodontitis, a major cause of tooth loss. This study evaluated the potential of an on-demand aqueous chlorine dioxide solution, “matching transformation system” (MA-T), as a safer alternative to conventional root canal irrigants. The antibacterial activity of MA-T was assessed against mono-species biofilms (Enterococcus faecalis, Parvimonas micra, Fusobacterium nucleatum), polymicrobial biofilms derived from human supragingival plaque, and an in vitro infected root canal model using E. faecalis. Antibacterial efficacy was evaluated using viable cell counting and confocal laser scanning microscopy (CLSM) to quantify the reduction in bacterial load. Biofilm mass was assessed by crystal violet (CV) staining. Cytotoxicity was evaluated by treating rat osteoblasts and human periodontal ligament fibroblasts with MA-T or NaOCl, followed by assessment of ATP levels and morphological integrity. MA-T eradicated planktonic bacteria, inhibited biofilm formation, and significantly reduced viable bacteria in established biofilms without affecting the total amount of biofilm biomass time-dependently. The infected root canal model showed a substantial decrease in bacterial load. Compared with NaOCl, MA-T-treated cells retained higher ATP levels and normal morphology. MA-T could be a promising root canal irrigant offering effective disinfection and superior biocompatibility, making it a potential alternative to traditional root canal irrigants as NaOCl.
出版者	Nature Research
収録物名	Scientific Reports
巻(号)	15
ページ	36215
刊行年月	2025-10-16
言語	英語
ハンドルURL	https://hdl.handle.net/11094/103668
eISSN	20452322
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1038/s41598-025-20131-5
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Scientific Reports. 2025, 15, p. 36215
DC.title	Application of on-demand aqueous chlorine dioxide solution for non-surgical root canal treatment
DC.creator	Shimaoka, Tsuyoshi
	Maezono, Hazuki
	Ono, Shunka
	Asahi, Yoko
	Kawanishi, Yuzo
	Klanliang, Kittipit
	Takahashi, Yusuke
	Hayashi, Mikako
DC.publisher	Nature Research
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2025-10-16
DC.identifier	https://doi.org/10.1038/s41598-025-20131-5
DC.subject	Biofilm
	Chlorine dioxide
	Cytotoxicity
	MA-T
	Osteoblast
	Periodontal ligament fibroblast
	Root canal irrigation
DC.description	Shimaoka T., Maezono H., Ono S., et al. Application of on-demand aqueous chlorine dioxide solution for non-surgical root canal treatment. Scientific Reports 15, 36215 (2025); https://doi.org/10.1038/s41598-025-20131-5.
DCTERMS.abstract	Oral biofilms contribute to apical periodontitis, a major cause of tooth loss. This study evaluated the potential of an on-demand aqueous chlorine dioxide solution, “matching transformation system” (MA-T), as a safer alternative to conventional root canal irrigants. The antibacterial activity of MA-T was assessed against mono-species biofilms (Enterococcus faecalis, Parvimonas micra, Fusobacterium nucleatum), polymicrobial biofilms derived from human supragingival plaque, and an in vitro infected root canal model using E. faecalis. Antibacterial efficacy was evaluated using viable cell counting and confocal laser scanning microscopy (CLSM) to quantify the reduction in bacterial load. Biofilm mass was assessed by crystal violet (CV) staining. Cytotoxicity was evaluated by treating rat osteoblasts and human periodontal ligament fibroblasts with MA-T or NaOCl, followed by assessment of ATP levels and morphological integrity. MA-T eradicated planktonic bacteria, inhibited biofilm formation, and significantly reduced viable bacteria in established biofilms without affecting the total amount of biofilm biomass time-dependently. The infected root canal model showed a substantial decrease in bacterial load. Compared with NaOCl, MA-T-treated cells retained higher ATP levels and normal morphology. MA-T could be a promising root canal irrigant offering effective disinfection and superior biocompatibility, making it a potential alternative to traditional root canal irrigants as NaOCl.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	103668
citation_title	Application of on-demand aqueous chlorine dioxide solution for non-surgical root canal treatment
citation_author	Shimaoka, Tsuyoshi
	Maezono, Hazuki
	Ono, Shunka
	Asahi, Yoko
	Kawanishi, Yuzo
	Klanliang, Kittipit
	Takahashi, Yusuke
	Hayashi, Mikako
citation_publisher	Nature Research
citation_language	英語
citation_date	2025-10-16
citation_journal_title	Scientific Reports
citation_volume	15
citation_firstpage	36215
citation_doi	https://doi.org/10.1038/s41598-025-20131-5
citation_public_url	https://hdl.handle.net/11094/103668

Application of on-demand aqueous chlorine dioxide solution for non-surgical root canal treatment

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