Autonomous Bioluminescence Systems: From Molecular Mechanisms to Emerging Applications

Kusuma, Subhan Hadi; Hattori, Mitsuru; Nagai, Takeharu

doi:https://doi.org/10.1021/jacsau.5c01072

Autonomous Bioluminescence Systems: From Molecular Mechanisms to Emerging Applications

Kusuma, Subhan Hadi; Hattori, Mitsuru; Nagai, Takeharu

JACS Au, 2025, 5(11), 5237-5252

アクセス数:8件（2026-02-15 18:42 集計）

固定URL: https://hdl.handle.net/11094/103673

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タイトル	Autonomous Bioluminescence Systems: From Molecular Mechanisms to Emerging Applications
著者	ORCID 0000-0002-5530-3901 0000-0002-5530-3901 著者名 Kusuma, Subhan Hadi
	NRID 1000020589858 1000020589858 ORCID 0000-0003-1189-6554 0000-0003-1189-6554 researchmap permalink mhatto mhatto Scopus Author ID 7401882679 7401882679 著者名 Hattori, Mitsuru
	NRID 1000020311350 1000020311350 ORCID 0000-0003-2650-9895 0000-0003-2650-9895 researchmap permalink ng1 ng1 Scopus Author ID 7403433377 7403433377 著者名 Nagai, Takeharu
キーワード等	autonomous bioluminescence systems
	bacterial luciferase (lux)
	bioimaging
	bioluminescence
	biosensors
	fungal luciferase (luz)
内容	Kusuma S.H., Hattori M., Nagai T.. Autonomous Bioluminescence Systems: From Molecular Mechanisms to Emerging Applications. JACS Au 5, 5237 (2025); https://doi.org/10.1021/jacsau.5c01072.
抄録	Autonomous bioluminescence systems─genetically encoded platforms that integrate luciferase enzymes with complete substrate biosynthetic pathways─have emerged as transformative tools for real-time, noninvasive imaging in living systems. Unlike conventional substrate-dependent bioluminescence, these systems provide continuous light emission without external substrates, enabling long-term monitoring with minimal phototoxicity compared to the use of fluorescence. Here, we present a critical perspective on recent advances in the two best-characterized autonomous systems: bacterial and fungal bioluminescence systems. We assess their molecular mechanisms, protein engineering strategies, and emerging applications in single-cell imaging, multicolor biosensing, and whole-organism monitoring. By comparing their strengths and limitations, we highlight persistent challenges, such as low quantum yield in bacterial bioluminescence and substrate availability constraints in fungal bioluminescence, and discuss strategies to address them─including AI-guided mutagenesis, de novo protein design, and metabolic pathway optimization. We conclude by outlining application-driven design targets for the next generation of autonomous bioluminescent systems in biomedical research, environmental monitoring, and synthetic biology.
出版者	American Chemical Society
収録物名	JACS Au
巻(号)	5(11)
ページ	5237-5252
刊行年月	2025-10-18
言語	英語
ハンドルURL	https://hdl.handle.net/11094/103673
eISSN	26913704
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1021/jacsau.5c01072
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	JACS Au. 2025, 5(11), p. 5237-5252
DC.title	Autonomous Bioluminescence Systems: From Molecular Mechanisms to Emerging Applications
DC.creator	Kusuma, Subhan Hadi
	Hattori, Mitsuru
	Nagai, Takeharu
DC.publisher	American Chemical Society
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2025-10-18
DC.identifier	https://doi.org/10.1021/jacsau.5c01072
DC.subject	autonomous bioluminescence systems
	bacterial luciferase (lux)
	bioimaging
	bioluminescence
	biosensors
	fungal luciferase (luz)
DC.description	Kusuma S.H., Hattori M., Nagai T.. Autonomous Bioluminescence Systems: From Molecular Mechanisms to Emerging Applications. JACS Au 5, 5237 (2025); https://doi.org/10.1021/jacsau.5c01072.
DCTERMS.abstract	Autonomous bioluminescence systems─genetically encoded platforms that integrate luciferase enzymes with complete substrate biosynthetic pathways─have emerged as transformative tools for real-time, noninvasive imaging in living systems. Unlike conventional substrate-dependent bioluminescence, these systems provide continuous light emission without external substrates, enabling long-term monitoring with minimal phototoxicity compared to the use of fluorescence. Here, we present a critical perspective on recent advances in the two best-characterized autonomous systems: bacterial and fungal bioluminescence systems. We assess their molecular mechanisms, protein engineering strategies, and emerging applications in single-cell imaging, multicolor biosensing, and whole-organism monitoring. By comparing their strengths and limitations, we highlight persistent challenges, such as low quantum yield in bacterial bioluminescence and substrate availability constraints in fungal bioluminescence, and discuss strategies to address them─including AI-guided mutagenesis, de novo protein design, and metabolic pathway optimization. We conclude by outlining application-driven design targets for the next generation of autonomous bioluminescent systems in biomedical research, environmental monitoring, and synthetic biology.
DC.rights	This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
DC.format	103673
citation_title	Autonomous Bioluminescence Systems: From Molecular Mechanisms to Emerging Applications
citation_author	Kusuma, Subhan Hadi
	Hattori, Mitsuru
	Nagai, Takeharu
citation_publisher	American Chemical Society
citation_language	英語
citation_date	2025-10-18
citation_journal_title	JACS Au
citation_volume	5
citation_issue	11
citation_firstpage	5237
citation_lastpage	5252
citation_doi	https://doi.org/10.1021/jacsau.5c01072
citation_public_url	https://hdl.handle.net/11094/103673

Autonomous Bioluminescence Systems: From Molecular Mechanisms to Emerging Applications

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