Comparative omics profiling reveals differences in biomass, energy production, and vesicle transport between CHO and fast-growing CHL-YN cells

Tsunoda, Yu; Arishima, Rintaro; Boronina, Tatiana; Cole, Robert; Yamano-Adachi, Noriko; Betenbaugh, Michael; Omasa, Takeshi

doi:https://doi.org/10.1038/s41598-025-23503-z

Comparative omics profiling reveals differences in biomass, energy production, and vesicle transport between CHO and fast-growing CHL-YN cells

Tsunoda, Yu; Arishima, Rintaro; Boronina, Tatiana et al.

Scientific Reports, 2025, 15, 39784

アクセス数:61件（2026-02-15 21:49 集計）

固定URL: https://hdl.handle.net/11094/103678

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タイトル	Comparative omics profiling reveals differences in biomass, energy production, and vesicle transport between CHO and fast-growing CHL-YN cells
著者	ORCID 0009-0008-9245-2835 0009-0008-9245-2835 Scopus Author ID 59146801800 59146801800 著者名 Tsunoda, Yu
	著者名 Arishima, Rintaro
	著者名 Boronina, Tatiana
	著者名 Cole, Robert
	NRID 1000020582795 1000020582795 ORCID 0000-0002-2167-5379 0000-0002-2167-5379 researchmap permalink kibou kibou Scopus Author ID 15137739600 15137739600 著者名 Yamano-Adachi, Noriko
	著者名 Betenbaugh, Michael
	NRID 1000000252586 1000000252586 ORCID 0000-0003-0635-3862 0000-0003-0635-3862 researchmap permalink omasa omasa Scopus Author ID 7003926741 7003926741 著者名 Omasa, Takeshi
キーワード等	Chinese hamster ovary cell
	Proteomics
	Transcriptomics
内容	Tsunoda Y., Arishima R., Boronina T., et al. Comparative omics profiling reveals differences in biomass, energy production, and vesicle transport between CHO and fast-growing CHL-YN cells. Scientific Reports 15, 39784 (2025); https://doi.org/10.1038/s41598-025-23503-z.
抄録	Chinese hamster lung (CHL)-YN cells are promising novel hosts for producing therapeutic antibodies with the potential to shorten the research, development, and manufacturing timelines in biopharmaceutical production. CHL-YN cells grow twice as fast as Chinese hamster ovary (CHO) cells, with a doubling time of 8.1 h. These cells possess strong glutamine synthetase activity, allowing them to be cultured in glutamine-free media. In this study, we conducted comparative transcriptomics and proteomics among CHL-YN cells, CHO cells, and lung tissue from Chinese hamster to better understand the global characteristics of CHL-YN cells and determine whether these features are tissue-derived or unique to the cell line. Omics profiling revealed that CHL-YN cells, in contrast to CHO cells, exhibit highly activated processes and pathways related to biomass and energy production, such as translation and biosynthesis of amino acids, but less activated vesicle transport processes, such as Golgi-related vesicle transport. This study highlights distinct characteristics of CHL-YN cells, contributing to streamlined operations, shortened development timelines, and expanded options for selectable cell lines. The findings here could contribute to identifying potential biomarkers and targets for cell engineering toward improving antibody productivity and growth rate in both traditional CHO cells and next-generation host cells, CHL-YN.
出版者	Nature Research
収録物名	Scientific Reports
巻(号)	15
ページ	39784
刊行年月	2025-11-13
言語	英語
ハンドルURL	https://hdl.handle.net/11094/103678
eISSN	20452322
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1038/s41598-025-23503-z
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Scientific Reports. 2025, 15, p. 39784
DC.title	Comparative omics profiling reveals differences in biomass, energy production, and vesicle transport between CHO and fast-growing CHL-YN cells
DC.creator	Tsunoda, Yu
	Arishima, Rintaro
	Boronina, Tatiana
	Cole, Robert
	Yamano-Adachi, Noriko
	Betenbaugh, Michael
	Omasa, Takeshi
DC.publisher	Nature Research
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2025-11-13
DC.identifier	https://doi.org/10.1038/s41598-025-23503-z
DC.subject	Chinese hamster ovary cell
	Proteomics
	Transcriptomics
DC.description	Tsunoda Y., Arishima R., Boronina T., et al. Comparative omics profiling reveals differences in biomass, energy production, and vesicle transport between CHO and fast-growing CHL-YN cells. Scientific Reports 15, 39784 (2025); https://doi.org/10.1038/s41598-025-23503-z.
DCTERMS.abstract	Chinese hamster lung (CHL)-YN cells are promising novel hosts for producing therapeutic antibodies with the potential to shorten the research, development, and manufacturing timelines in biopharmaceutical production. CHL-YN cells grow twice as fast as Chinese hamster ovary (CHO) cells, with a doubling time of 8.1 h. These cells possess strong glutamine synthetase activity, allowing them to be cultured in glutamine-free media. In this study, we conducted comparative transcriptomics and proteomics among CHL-YN cells, CHO cells, and lung tissue from Chinese hamster to better understand the global characteristics of CHL-YN cells and determine whether these features are tissue-derived or unique to the cell line. Omics profiling revealed that CHL-YN cells, in contrast to CHO cells, exhibit highly activated processes and pathways related to biomass and energy production, such as translation and biosynthesis of amino acids, but less activated vesicle transport processes, such as Golgi-related vesicle transport. This study highlights distinct characteristics of CHL-YN cells, contributing to streamlined operations, shortened development timelines, and expanded options for selectable cell lines. The findings here could contribute to identifying potential biomarkers and targets for cell engineering toward improving antibody productivity and growth rate in both traditional CHO cells and next-generation host cells, CHL-YN.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	103678
citation_title	Comparative omics profiling reveals differences in biomass, energy production, and vesicle transport between CHO and fast-growing CHL-YN cells
citation_author	Tsunoda, Yu
	Arishima, Rintaro
	Boronina, Tatiana
	Cole, Robert
	Yamano-Adachi, Noriko
	Betenbaugh, Michael
	Omasa, Takeshi
citation_publisher	Nature Research
citation_language	英語
citation_date	2025-11-13
citation_journal_title	Scientific Reports
citation_volume	15
citation_firstpage	39784
citation_doi	https://doi.org/10.1038/s41598-025-23503-z
citation_public_url	https://hdl.handle.net/11094/103678

Comparative omics profiling reveals differences in biomass, energy production, and vesicle transport between CHO and fast-growing CHL-YN cells

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