Neutrophil gene expression in COVID-19 patients with acute respiratory distress syndrome

Ito, Hiroshi; Ishikawa, Masakazu; Yoshimura, Jumpei; Liu, Yuchen; Sakakibara, Shuhei; Sugihara, Fuminori; Matsumoto, Hisatake; Hirata, Haruhiko; Ogura, Hiroshi; Oda, Jun; Okuzaki, Daisuke

doi:https://doi.org/10.3389/fimmu.2025.1620745

Neutrophil gene expression in COVID-19 patients with acute respiratory distress syndrome

Ito, Hiroshi; Ishikawa, Masakazu; Yoshimura, Jumpei et al.

Frontiers in Immunology, 2025, 16, 1620745

アクセス数:28件（2026-02-16 10:17 集計）

固定URL: https://hdl.handle.net/11094/103687

閲覧可能ファイル

ファイル	フォーマット	利用条件	サイズ	閲覧回数	利用開始日	説明	information
FrontImmunol_16_1620745	pdf	なし	3.84 MB	4	2026.01.30

Creative Commons : 表示

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

タイトル	Neutrophil gene expression in COVID-19 patients with acute respiratory distress syndrome
著者	NRID 1000080836594 1000080836594 ORCID 0000-0002-7525-5273 0000-0002-7525-5273 researchmap permalink 1009hiroshi.ito 1009hiroshi.ito Scopus Author ID 57754951900 57754951900 著者名 Ito, Hiroshi
	NRID 1000010897028 1000010897028 著者名 Ishikawa, Masakazu
	NRID 1000030835202 1000030835202 著者名 Yoshimura, Jumpei
	NRID 1000050894698 1000050894698 ORCID 0000-0002-2750-0182 0000-0002-2750-0182 researchmap permalink liuyuchen liuyuchen 著者名 Liu, Yuchen
	NRID 1000010618838 1000010618838 ORCID 0000-0003-3157-5870 0000-0003-3157-5870 researchmap permalink SSakakibara SSakakibara 著者名 Sakakibara, Shuhei
	NRID 1000050381645 1000050381645 ORCID 0000-0002-6228-4593 0000-0002-6228-4593 researchmap permalink sugiharaf sugiharaf Scopus Author ID 12809512100 12809512100 著者名 Sugihara, Fuminori
	NRID 1000070644003 1000070644003 ORCID 0000-0001-5771-570X 0000-0001-5771-570X researchmap permalink 38283828 38283828 Scopus Author ID 36632584800 36632584800 著者名 Matsumoto, Hisatake
	NRID 1000030546867 1000030546867 researchmap permalink hh_resmed hh_resmed Scopus Author ID 7202342767 7202342767 著者名 Hirata, Haruhiko
	NRID 1000070301265 1000070301265 researchmap permalink read0054647 read0054647 Scopus Author ID 7202495132 7202495132 著者名 Ogura, Hiroshi
	NRID 1000060459500 1000060459500 ORCID 0000-0002-8254-3716 0000-0002-8254-3716 researchmap permalink read0068529 read0068529 Scopus Author ID 56210537000 56210537000 著者名 Oda, Jun
	NRID 1000000346131 1000000346131 ORCID 0000-0002-4552-783X 0000-0002-4552-783X researchmap permalink read0092307 read0092307 Scopus Author ID 6506703054 6506703054 著者名 Okuzaki, Daisuke
キーワード等	acute respiratory distress syndrome
	coronavirus disease 2019 (COVID-19)
	gene expression
	neutrophil
	single-cell RNA sequencing
内容	Ito H., Ishikawa M., Yoshimura J., et al. Neutrophil gene expression in COVID-19 patients with acute respiratory distress syndrome. Frontiers in Immunology 16, 1620745 (2025); https://doi.org/10.3389/fimmu.2025.1620745.
抄録	Background: Although an increase in neutrophil count has been observed in patients with coronavirus disease 2019 (COVID-19), the relationship between the systemic neutrophil transcriptome and clinical course of COVID-19 remains unclear. Hence, we examined the relationship between the clinical course and RNA sequencing analysis results in COVID-19 patients. Methods: Peripheral blood samples were obtained from 28 patients with COVID-19-associated ARDS and 16 healthy controls. Bulk RNA sequencing was performed, and clustering analysis was used to explore relationships between gene expression and clinical characteristics. In a separate cohort, neutrophils were isolated from the peripheral blood of five COVID-19 patients with ARDS for single-cell RNA sequencing to further characterize the neutrophil subpopulations. Results: In bulk RNA sequencing analysis, COVID-19 patients with ARDS had elevated gene expression associated with neutrophils compared with healthy controls.Clustering analysis revealed no differences in the clinical characteristics of COVID-19 patients with ARDS. In the single-cell RNA sequencing analysis, clustering analysis showed that the patients were divided into two groups: those who could be weaned from the ventilator within 28 days and those who could not be weaned. Conclusion: These findings indicate that differences in neutrophil gene expression may have important clinical implications. This study may support the exploratory identification of genomic factors, such as neutrophil gene expression, that are relevant to clinical parameters.
出版者	Frontiers Media SA
収録物名	Frontiers in Immunology
巻(号)	16
ページ	1620745
刊行年月	2025-11-06
言語	英語
ハンドルURL	https://hdl.handle.net/11094/103687
eISSN	16643224
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.3389/fimmu.2025.1620745
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Frontiers in Immunology. 2025, 16, p. 1620745
DC.title	Neutrophil gene expression in COVID-19 patients with acute respiratory distress syndrome
DC.creator	Ito, Hiroshi
	Ishikawa, Masakazu
	Yoshimura, Jumpei
	Liu, Yuchen
	Sakakibara, Shuhei
	Sugihara, Fuminori
	Matsumoto, Hisatake
	Hirata, Haruhiko
	Ogura, Hiroshi
	Oda, Jun
	Okuzaki, Daisuke
DC.publisher	Frontiers Media SA
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2025-11-06
DC.identifier	https://doi.org/10.3389/fimmu.2025.1620745
DC.subject	acute respiratory distress syndrome
	coronavirus disease 2019 (COVID-19)
	gene expression
	neutrophil
	single-cell RNA sequencing
DC.description	Ito H., Ishikawa M., Yoshimura J., et al. Neutrophil gene expression in COVID-19 patients with acute respiratory distress syndrome. Frontiers in Immunology 16, 1620745 (2025); https://doi.org/10.3389/fimmu.2025.1620745.
DCTERMS.abstract	Background: Although an increase in neutrophil count has been observed in patients with coronavirus disease 2019 (COVID-19), the relationship between the systemic neutrophil transcriptome and clinical course of COVID-19 remains unclear. Hence, we examined the relationship between the clinical course and RNA sequencing analysis results in COVID-19 patients. Methods: Peripheral blood samples were obtained from 28 patients with COVID-19-associated ARDS and 16 healthy controls. Bulk RNA sequencing was performed, and clustering analysis was used to explore relationships between gene expression and clinical characteristics. In a separate cohort, neutrophils were isolated from the peripheral blood of five COVID-19 patients with ARDS for single-cell RNA sequencing to further characterize the neutrophil subpopulations. Results: In bulk RNA sequencing analysis, COVID-19 patients with ARDS had elevated gene expression associated with neutrophils compared with healthy controls.Clustering analysis revealed no differences in the clinical characteristics of COVID-19 patients with ARDS. In the single-cell RNA sequencing analysis, clustering analysis showed that the patients were divided into two groups: those who could be weaned from the ventilator within 28 days and those who could not be weaned. Conclusion: These findings indicate that differences in neutrophil gene expression may have important clinical implications. This study may support the exploratory identification of genomic factors, such as neutrophil gene expression, that are relevant to clinical parameters.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	103687
citation_title	Neutrophil gene expression in COVID-19 patients with acute respiratory distress syndrome
citation_author	Ito, Hiroshi
	Ishikawa, Masakazu
	Yoshimura, Jumpei
	Liu, Yuchen
	Sakakibara, Shuhei
	Sugihara, Fuminori
	Matsumoto, Hisatake
	Hirata, Haruhiko
	Ogura, Hiroshi
	Oda, Jun
	Okuzaki, Daisuke
citation_publisher	Frontiers Media SA
citation_language	英語
citation_date	2025-11-06
citation_journal_title	Frontiers in Immunology
citation_volume	16
citation_firstpage	1620745
citation_doi	https://doi.org/10.3389/fimmu.2025.1620745
citation_public_url	https://hdl.handle.net/11094/103687

Neutrophil gene expression in COVID-19 patients with acute respiratory distress syndrome

閲覧可能ファイル

論文情報

論文詳細を表示