Multiple comprehensive analyses identify that upregulation of Myomesin 2 suppresses cardiomyocyte proliferation

Suzuki, Shota; Tanaka, Shota; Wada, Yuriko; Kametani, Yusuke; Sakai, Hibiki; Nishinaka, Kosuke; Egawa, Kaho; Okada, Yoshiaki; Obana, Masanori; Fujio, Yasushi

doi:https://doi.org/10.1016/j.bbrc.2026.154068

Multiple comprehensive analyses identify that upregulation of Myomesin 2 suppresses cardiomyocyte proliferation

Suzuki, Shota; Tanaka, Shota; Wada, Yuriko et al.

Biochemical and Biophysical Research Communications, 2026, 828, 154068

アクセス数:204件（2026-08-24 15:14 集計）

固定URL: https://hdl.handle.net/11094/105848

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タイトル	Multiple comprehensive analyses identify that upregulation of Myomesin 2 suppresses cardiomyocyte proliferation
著者	著者名 Suzuki, Shota
	NRID 1000080880294 1000080880294 ORCID 0000-0002-3053-6928 0000-0002-3053-6928 researchmap permalink 80880294 80880294 Scopus Author ID 56612561000 56612561000 著者名 Tanaka, Shota
	著者名 Wada, Yuriko
	著者名 Kametani, Yusuke
	著者名 Sakai, Hibiki
	著者名 Nishinaka, Kosuke
	著者名 Egawa, Kaho
	NRID 1000050444500 1000050444500 ORCID 0000-0002-2962-244X 0000-0002-2962-244X researchmap permalink magic_roundabout magic_roundabout Scopus Author ID 55721995000 55721995000 著者名 Okada, Yoshiaki
	NRID 1000050745883 1000050745883 researchmap permalink m_o m_o Scopus Author ID 17135691100 17135691100 著者名 Obana, Masanori
	NRID 1000020359839 1000020359839 ORCID 0000-0003-0828-2930 0000-0003-0828-2930 researchmap permalink yfujio yfujio Scopus Author ID 7006517527 7006517527 著者名 Fujio, Yasushi
キーワード等	Cardiomyocytes
	Cell proliferation
	RNA-Seq
	Sarcomeres
	Single-cell analysis
内容	Suzuki S., Tanaka S., Wada Y., et al. Multiple comprehensive analyses identify that upregulation of Myomesin 2 suppresses cardiomyocyte proliferation. Biochemical and Biophysical Research Communications 828, 154068 (2026); https://doi.org/10.1016/j.bbrc.2026.154068.
抄録	Mammalian cardiomyocytes lose their proliferative capacity shortly after birth; however, molecular mechanisms that regulate cardiomyocyte proliferation remain to be fully elucidated. This study aimed to identify genes that regulate cardiomyocyte proliferation through multiple comprehensive analyses. RNA-sequencing analysis was performed using proliferating neonatal rat cardiomyocytes. We also retrieved and analyzed publicly available single-cell RNA-sequencing datasets from rat hearts on postnatal days 1 and 7. Pseudotime trajectory analysis identified a cardiomyocyte cluster undergoing cell cycle exit at postnatal day 7. Comparative gene expression analysis of this cluster between postnatal day 1 and day 7 identified Myomesin 2 (Myom2) as a candidate for regulation of cardiomyocyte proliferation. Real time qRT-PCR analysis demonstrated that Myom2 expression increased during cardiac maturation. Further, we investigated the effect of Myom2 expression on cardiomyocyte proliferation. Immunofluorescence microscopic analysis revealed that adenoviral overexpression of Myom2 significantly reduced the proportion of proliferating cardiomyocytes and promoted cardiomyocyte binucleation. Collectively, these findings indicate that postnatal induction of Myom2 suppresses cardiomyocyte proliferation and promotes binucleation.
出版者	Elsevier B.V.
収録物名	Biochemical and Biophysical Research Communications
巻(号)	828
ページ	154068
刊行年月	2026-08-27
言語	英語
ハンドルURL	https://hdl.handle.net/11094/105848
PISSN	0006291X
eISSN	10902104
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1016/j.bbrc.2026.154068
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Biochemical and Biophysical Research Communications. 2026, 828, p. 154068
DC.title	Multiple comprehensive analyses identify that upregulation of Myomesin 2 suppresses cardiomyocyte proliferation
DC.creator	Suzuki, Shota
	Tanaka, Shota
	Wada, Yuriko
	Kametani, Yusuke
	Sakai, Hibiki
	Nishinaka, Kosuke
	Egawa, Kaho
	Okada, Yoshiaki
	Obana, Masanori
	Fujio, Yasushi
DC.publisher	Elsevier B.V.
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2026-08-27
DC.identifier	https://doi.org/10.1016/j.bbrc.2026.154068
DC.subject	Cardiomyocytes
	Cell proliferation
	RNA-Seq
	Sarcomeres
	Single-cell analysis
DC.description	Suzuki S., Tanaka S., Wada Y., et al. Multiple comprehensive analyses identify that upregulation of Myomesin 2 suppresses cardiomyocyte proliferation. Biochemical and Biophysical Research Communications 828, 154068 (2026); https://doi.org/10.1016/j.bbrc.2026.154068.
DCTERMS.abstract	Mammalian cardiomyocytes lose their proliferative capacity shortly after birth; however, molecular mechanisms that regulate cardiomyocyte proliferation remain to be fully elucidated. This study aimed to identify genes that regulate cardiomyocyte proliferation through multiple comprehensive analyses. RNA-sequencing analysis was performed using proliferating neonatal rat cardiomyocytes. We also retrieved and analyzed publicly available single-cell RNA-sequencing datasets from rat hearts on postnatal days 1 and 7. Pseudotime trajectory analysis identified a cardiomyocyte cluster undergoing cell cycle exit at postnatal day 7. Comparative gene expression analysis of this cluster between postnatal day 1 and day 7 identified Myomesin 2 (Myom2) as a candidate for regulation of cardiomyocyte proliferation. Real time qRT-PCR analysis demonstrated that Myom2 expression increased during cardiac maturation. Further, we investigated the effect of Myom2 expression on cardiomyocyte proliferation. Immunofluorescence microscopic analysis revealed that adenoviral overexpression of Myom2 significantly reduced the proportion of proliferating cardiomyocytes and promoted cardiomyocyte binucleation. Collectively, these findings indicate that postnatal induction of Myom2 suppresses cardiomyocyte proliferation and promotes binucleation.
DC.rights	This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
DC.format	105848
citation_title	Multiple comprehensive analyses identify that upregulation of Myomesin 2 suppresses cardiomyocyte proliferation
citation_author	Suzuki, Shota
	Tanaka, Shota
	Wada, Yuriko
	Kametani, Yusuke
	Sakai, Hibiki
	Nishinaka, Kosuke
	Egawa, Kaho
	Okada, Yoshiaki
	Obana, Masanori
	Fujio, Yasushi
citation_publisher	Elsevier B.V.
citation_language	英語
citation_date	2026-08-27
citation_journal_title	Biochemical and Biophysical Research Communications
citation_volume	828
citation_firstpage	154068
citation_doi	https://doi.org/10.1016/j.bbrc.2026.154068
citation_public_url	https://hdl.handle.net/11094/105848

Multiple comprehensive analyses identify that upregulation of Myomesin 2 suppresses cardiomyocyte proliferation

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